Legends: 1, BINDING NADP. {ECO:0000269|PubMed:15327949}; 2, NP_BIND NADP. {ECO:0000269|PubMed:15327949}; 3, ipat:ADH_IRON_1 [T]; 4, ipat:ADH_IRON_2 [T]; 5, STRAND {ECO:0000244|PDB:4QGS}; 6, HELIX {ECO:0000244|PDB:4QGS}; 7, TURN {ECO:0000244|PDB:4QGS}; 8, HELIX {ECO:0000244|PDB:1OJ7}; 9, TURN {ECO:0000244|PDB:1OJ7}; 10, STRAND {ECO:0000244|PDB:1OJ7}.
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ID YQHD_ECOLI Reviewed; 387 AA.
AC Q46856; Q2M9I8;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 02-NOV-2016, entry version 111.
DE RecName: Full=Alcohol dehydrogenase YqhD;
DE EC=1.1.1.-;
GN Name=yqhD; OrderedLocusNames=b3011, JW2978;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-387 IN COMPLEX WITH ZINC
RP IONS AND NADP, FUNCTION, AND SUBUNIT.
RX PubMed=15327949; DOI=10.1016/j.jmb.2004.07.034;
RA Sulzenbacher G., Alvarez K., Van Den Heuvel R.H., Versluis C.,
RA Spinelli S., Campanacci V., Valencia C., Cambillau C., Eklund H.,
RA Tegoni M.;
RT "Crystal structure of E.coli alcohol dehydrogenase YqhD: evidence of a
RT covalently modified NADP coenzyme.";
RL J. Mol. Biol. 342:489-502(2004).
CC -!- FUNCTION: NADP-dependent ADH activity.
CC {ECO:0000269|PubMed:15327949}.
CC -!- CATALYTIC ACTIVITY: An alcohol + NADP(+) = an aldehyde + NADPH.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Note=Binds 1 zinc ion per subunit.;
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:15327949}.
CC -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC family. {ECO:0000305}.
DR EMBL; U28377; AAA69178.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76047.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77068.1; -; Genomic_DNA.
DR PIR; A65088; A65088.
DR RefSeq; NP_417484.1; NC_000913.3.
DR RefSeq; WP_001058802.1; NZ_LN832404.1.
DR PDB; 1OJ7; X-ray; 2.00 A; A/B/C/D=2-387.
DR PDB; 4QGS; X-ray; 1.70 A; A=1-387.
DR PDBsum; 1OJ7; -.
DR PDBsum; 4QGS; -.
DR ProteinModelPortal; Q46856; -.
DR SMR; Q46856; -.
DR BioGrid; 4262380; 12.
DR DIP; DIP-12870N; -.
DR IntAct; Q46856; 3.
DR STRING; 511145.b3011; -.
DR PaxDb; Q46856; -.
DR PRIDE; Q46856; -.
DR EnsemblBacteria; AAC76047; AAC76047; b3011.
DR EnsemblBacteria; BAE77068; BAE77068; BAE77068.
DR GeneID; 947493; -.
DR KEGG; ecj:JW2978; -.
DR KEGG; eco:b3011; -.
DR PATRIC; 32121438; VBIEscCol129921_3106.
DR EchoBASE; EB2834; -.
DR EcoGene; EG13014; yqhD.
DR eggNOG; ENOG4108I6A; Bacteria.
DR eggNOG; COG1979; LUCA.
DR HOGENOM; HOG000243334; -.
DR InParanoid; Q46856; -.
DR KO; K08325; -.
DR OMA; SYETLMN; -.
DR PhylomeDB; Q46856; -.
DR BioCyc; EcoCyc:G7564-MONOMER; -.
DR BioCyc; ECOL316407:JW2978-MONOMER; -.
DR BioCyc; MetaCyc:G7564-MONOMER; -.
DR SABIO-RK; Q46856; -.
DR EvolutionaryTrace; Q46856; -.
DR PRO; PR:Q46856; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0008106; F:alcohol dehydrogenase (NADP+) activity; IDA:EcoCyc.
DR GO; GO:0018455; F:alcohol dehydrogenase [NAD(P)+] activity; IDA:EcoliWiki.
DR GO; GO:0033721; F:aldehyde dehydrogenase (NADP+) activity; IDA:EcoCyc.
DR GO; GO:1990002; F:methylglyoxal reductase (NADPH-dependent, acetol producing); IDA:EcoCyc.
DR GO; GO:0008270; F:zinc ion binding; IDA:EcoCyc.
DR GO; GO:0000302; P:response to reactive oxygen species; IMP:EcoCyc.
DR InterPro; IPR001670; ADH_Fe.
DR InterPro; IPR018211; ADH_Fe_CS.
DR Pfam; PF00465; Fe-ADH; 1.
DR PROSITE; PS00913; ADH_IRON_1; 1.
DR PROSITE; PS00060; ADH_IRON_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Metal-binding; NADP; Oxidoreductase;
KW Reference proteome; Zinc.
FT CHAIN 1 387 Alcohol dehydrogenase YqhD.
FT /FTId=PRO_0000087832.
FT NP_BIND 38 40 NADP. {ECO:0000269|PubMed:15327949}.
FT NP_BIND 93 99 NADP. {ECO:0000269|PubMed:15327949}.
FT NP_BIND 179 182 NADP. {ECO:0000269|PubMed:15327949}.
FT METAL 194 194 Zinc; catalytic.
FT METAL 198 198 Zinc; catalytic.
FT METAL 267 267 Zinc; catalytic.
FT METAL 281 281 Zinc; catalytic.
FT BINDING 138 138 NADP. {ECO:0000269|PubMed:15327949}.
FT BINDING 147 147 NADP. {ECO:0000269|PubMed:15327949}.
FT BINDING 160 160 NADP. {ECO:0000269|PubMed:15327949}.
FT STRAND 11 15 {ECO:0000244|PDB:4QGS}.
FT HELIX 18 21 {ECO:0000244|PDB:4QGS}.
FT HELIX 23 25 {ECO:0000244|PDB:4QGS}.
FT STRAND 31 35 {ECO:0000244|PDB:4QGS}.
FT STRAND 37 39 {ECO:0000244|PDB:4QGS}.
FT HELIX 40 44 {ECO:0000244|PDB:4QGS}.
FT HELIX 46 53 {ECO:0000244|PDB:4QGS}.
FT TURN 54 56 {ECO:0000244|PDB:4QGS}.
FT STRAND 57 62 {ECO:0000244|PDB:4QGS}.
FT HELIX 71 84 {ECO:0000244|PDB:4QGS}.
FT STRAND 88 94 {ECO:0000244|PDB:4QGS}.
FT HELIX 95 108 {ECO:0000244|PDB:4QGS}.
FT HELIX 118 121 {ECO:0000244|PDB:4QGS}.
FT TURN 122 126 {ECO:0000244|PDB:4QGS}.
FT STRAND 133 139 {ECO:0000244|PDB:4QGS}.
FT HELIX 144 146 {ECO:0000244|PDB:1OJ7}.
FT STRAND 150 153 {ECO:0000244|PDB:4QGS}.
FT TURN 155 158 {ECO:0000244|PDB:1OJ7}.
FT STRAND 161 163 {ECO:0000244|PDB:4QGS}.
FT TURN 166 168 {ECO:0000244|PDB:4QGS}.
FT STRAND 171 175 {ECO:0000244|PDB:4QGS}.
FT HELIX 177 180 {ECO:0000244|PDB:4QGS}.
FT HELIX 185 200 {ECO:0000244|PDB:4QGS}.
FT TURN 201 203 {ECO:0000244|PDB:4QGS}.
FT STRAND 204 206 {ECO:0000244|PDB:1OJ7}.
FT HELIX 211 233 {ECO:0000244|PDB:4QGS}.
FT HELIX 238 251 {ECO:0000244|PDB:4QGS}.
FT STRAND 252 254 {ECO:0000244|PDB:1OJ7}.
FT TURN 255 259 {ECO:0000244|PDB:4QGS}.
FT HELIX 265 277 {ECO:0000244|PDB:4QGS}.
FT HELIX 281 295 {ECO:0000244|PDB:4QGS}.
FT TURN 296 300 {ECO:0000244|PDB:4QGS}.
FT HELIX 301 312 {ECO:0000244|PDB:4QGS}.
FT HELIX 319 336 {ECO:0000244|PDB:4QGS}.
FT HELIX 343 346 {ECO:0000244|PDB:4QGS}.
FT HELIX 353 362 {ECO:0000244|PDB:4QGS}.
FT STRAND 366 368 {ECO:0000244|PDB:4QGS}.
FT TURN 369 372 {ECO:0000244|PDB:4QGS}.
FT HELIX 376 385 {ECO:0000244|PDB:4QGS}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 173 201 ipat:ADH_IRON_1 [T]
FT MYHIT 264 284 ipat:ADH_IRON_2 [T]
FT MYHIT 9 360 ipfam:Fe-ADH [T]
SQ SEQUENCE 387 AA; 42097 MW; 448A7610787D2146 CRC64;
MNNFNLHTPT RILFGKGAIA GLREQIPHDA RVLITYGGGS VKKTGVLDQV LDALKGMDVL
EFGGIEPNPA YETLMNAVKL VREQKVTFLL AVGGGSVLDG TKFIAAAANY PENIDPWHIL
QTGGKEIKSA IPMGCVLTLP ATGSESNAGA VISRKTTGDK QAFHSAHVQP VFAVLDPVYT
YTLPPRQVAN GVVDAFVHTV EQYVTKPVDA KIQDRFAEGI LLTLIEDGPK ALKEPENYDV
RANVMWAATQ ALNGLIGAGV PQDWATHMLG HELTAMHGLD HAQTLAIVLP ALWNEKRDTK
RAKLLQYAER VWNITEGSDD ERIDAAIAAT RNFFEQLGVP THLSDYGLDG SSIPALLKKL
EEHGMTQLGE NHDITLDVSR RIYEAAR
//
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