Legends: 1, ACT_SITE Nucleophile. {ECO:0000255|PROSITE- ProRule:PRU01024}; 2, Iron-sulfur (4Fe-4S). {ECO:0000250}; 3, BINDING S-adenosyl-L-methionine. {ECO:0000255|PROSITE-ProRule:PRU01024}; 4, BINDING S-adenosyl-L-methionine; via carbonyl oxygen. {ECO:0000255|PROSITE- ProRule:PRU01024}; 5, TRAM. {ECO:0000255|PROSITE- ProRule:PRU00208}; 6, ipfam:TRAM [T]; 7, ipfam:tRNA_U5-meth_tr [T]; 8, ipat:TRMA_1 [T]; 9, ipat:TRMA_2 [T].
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ID Y363_BACC1 Reviewed; 460 AA.
AC Q73EJ5;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 05-OCT-2016, entry version 78.
DE RecName: Full=Uncharacterized RNA methyltransferase BCE_0363;
DE EC=2.1.1.-;
GN OrderedLocusNames=BCE_0363;
OS Bacillus cereus (strain ATCC 10987 / NRS 248).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=222523;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10987 / NRS 248;
RX PubMed=14960714; DOI=10.1093/nar/gkh258;
RA Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L.,
RA Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F.,
RA Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D.;
RT "The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic
RT adaptations and a large plasmid related to Bacillus anthracis pXO1.";
RL Nucleic Acids Res. 32:977-988(2004).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding
CC methyltransferase superfamily. RNA M5U methyltransferase family.
CC {ECO:0000255|PROSITE-ProRule:PRU01024}.
CC -!- SIMILARITY: Contains 1 TRAM domain. {ECO:0000255|PROSITE-
CC ProRule:PRU00208}.
DR EMBL; AE017194; AAS39299.1; -; Genomic_DNA.
DR RefSeq; WP_000105047.1; NC_003909.8.
DR ProteinModelPortal; Q73EJ5; -.
DR EnsemblBacteria; AAS39299; AAS39299; BCE_0363.
DR KEGG; bca:BCE_0363; -.
DR PATRIC; 18849604; VBIBacCer118379_0349.
DR HOGENOM; HOG000029867; -.
DR KO; K03215; -.
DR OMA; WGEEYIY; -.
DR Proteomes; UP000002527; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR001566; 23S_rRNA_MeTrfase_RlmD.
DR InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR Pfam; PF02475; Met_10; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00479; rumA; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding;
KW Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1 460 Uncharacterized RNA methyltransferase
FT BCE_0363.
FT /FTId=PRO_0000161948.
FT DOMAIN 8 66 TRAM. {ECO:0000255|PROSITE-
FT ProRule:PRU00208}.
FT ACT_SITE 415 415 Nucleophile. {ECO:0000255|PROSITE-
FT ProRule:PRU01024}.
FT METAL 79 79 Iron-sulfur (4Fe-4S). {ECO:0000250}.
FT METAL 85 85 Iron-sulfur (4Fe-4S). {ECO:0000250}.
FT METAL 88 88 Iron-sulfur (4Fe-4S). {ECO:0000250}.
FT METAL 166 166 Iron-sulfur (4Fe-4S). {ECO:0000250}.
FT BINDING 290 290 S-adenosyl-L-methionine.
FT {ECO:0000255|PROSITE-ProRule:PRU01024}.
FT BINDING 319 319 S-adenosyl-L-methionine; via carbonyl
FT oxygen. {ECO:0000255|PROSITE-
FT ProRule:PRU01024}.
FT BINDING 340 340 S-adenosyl-L-methionine.
FT {ECO:0000255|PROSITE-ProRule:PRU01024}.
FT BINDING 388 388 S-adenosyl-L-methionine.
FT {ECO:0000255|PROSITE-ProRule:PRU01024}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 8 66 iprf:TRAM [T]
FT MYHIT 86 458 iprf:SAM_MT_RNA_M5U [T]
FT MYHIT 12 64 ipfam:TRAM [T]
FT MYHIT 383 457 ipfam:tRNA_U5-meth_tr [T]
FT MYHIT 211 360 ipfam:Met_10 [T]
FT MYHIT 388 419 ipat:TRMA_1 [T]
FT MYHIT 440 450 ipat:TRMA_2 [T]
SQ SEQUENCE 460 AA; 51618 MW; CDB4B9F7039EAFD3 CRC64;
MSTKMTPPVE KNEFIDVVFE DLTHDGAGVA KVKGYPIFVK NGLPGEEAQI KIIKVKKNFA
FGRLMKLHTE SPYRKDAECP VYNQCGGCQL QHLTYEGQLQ AKEKQVRDVM QRIGGLSDVP
VHPVLGMKNP WVYRNKAQVP IGEREGGLVA GFYRQGTHDI INMESCLIQA EENDTLIQEV
KRICEKHGIS AYNEERNKGT LRHVMARYGQ VTGEIMLVFI TRTAELPNKK AIIEEIATKF
PEVKSIVQNV NPKRTNVIFG DKTTVLYGSE YIYDFIGDIK FAISARSFYQ VNPEQTKVLY
DKTLEYAKLD GNETVIDAYC GIGSISLFLA QKAKKVYGVE IVPEAIEDAK RNAALNNMTN
AEFGVGEAEV VIPKWYKEGV IADTMVVDPP RKGCDEALLN TIIDMKPKRV VYVSCNPATL
ARDLKVLEEG GYKTQEVQPV DMFPHTTHVE CVVLMSRVEK
//
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