Entry sw:WALR_STAAU

ID   WALR_STAAU              Reviewed;         233 AA.
AC   Q9RDT5;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-NOV-2008, sequence version 2.
DT   18-JAN-2017, entry version 93.
DE   RecName: Full=Transcriptional regulatory protein WalR {ECO:0000305};
GN   Name=walR; Synonyms=vicR, yycF;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12379689; DOI=10.1128/IAI.70.11.6121-6128.2002;
RA   Wagner C., de Saizieu A., Schoenfeld H.-J., Kamber M., Lange R.,
RA   Thompson C.J., Page M.G.;
RT   "Genetic analysis and functional characterization of the Streptococcus
RT   pneumoniae vic operon.";
RL   Infect. Immun. 70:6121-6128(2002).
RN   [2]
RP   PHOSPHORYLATION.
RC   STRAIN=WCUH29 / NCIMB 40771;
RX   PubMed=12867749; DOI=10.1159/000071077;
RA   Clausen V.A., Bae W., Throup J., Burnham M.K.R., Rosenberg M.,
RA   Wallis N.G.;
RT   "Biochemical characterization of the first essential two-component
RT   signal transduction system from Staphylococcus aureus and
RT   Streptococcus pneumoniae.";
RL   J. Mol. Microbiol. Biotechnol. 5:252-260(2003).
RN   [3]
RP   OVEREXPRESSION.
RC   STRAIN=SA137/93A;
RX   PubMed=17418637; DOI=10.1016/j.ijmm.2007.02.002;
RA   Jansen A., Tuerck M., Szekat C., Nagel M., Clever I., Bierbaum G.;
RT   "Role of insertion elements and yycFG in the development of decreased
RT   susceptibility to vancomycin in Staphylococcus aureus.";
RL   Int. J. Med. Microbiol. 297:205-215(2007).
CC   -!- FUNCTION: Member of the two-component regulatory system WalK/WalR
CC       that regulates genes involved in autolysis, biofilm formation and
CC       cell wall metabolism. {ECO:0000250|UniProtKB:Q2G2U6}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by WalK. {ECO:0000269|PubMed:12867749}.
CC   -!- MISCELLANEOUS: Overexpression of walK/walR reduces susceptibility
CC       against vancomycin. {ECO:0000269|PubMed:17418637}.
CC   -!- SIMILARITY: Contains 1 OmpR/PhoB-type DNA-binding domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU01091}.
CC   -!- SIMILARITY: Contains 1 response regulatory domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00169}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB65399.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; AJ012052; CAB65399.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000101976.1; NZ_MLQK01000006.1.
DR   PDB; 2ZXJ; X-ray; 1.87 A; A/B=123-233.
DR   PDBsum; 2ZXJ; -.
DR   ProteinModelPortal; Q9RDT5; -.
DR   SMR; Q9RDT5; -.
DR   STRING; 93062.SACOL0019; -.
DR   GeneID; 28379619; -.
DR   eggNOG; ENOG4105CK6; Bacteria.
DR   eggNOG; COG0745; LUCA.
DR   EvolutionaryTrace; Q9RDT5; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR011991; WHTH_DNA-bd_dom.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cytoplasm; DNA-binding; Phosphoprotein;
KW   Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN         1    233       Transcriptional regulatory protein WalR.
FT                                /FTId=PRO_0000353031.
FT   DOMAIN        4    117       Response regulatory.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00169}.
FT   DNA_BIND    132    231       OmpR/PhoB-type. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01091}.
FT   MOD_RES      53     53       4-aspartylphosphate.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00169}.
FT   STRAND      135    137       {ECO:0000244|PDB:2ZXJ}.
FT   STRAND      140    143       {ECO:0000244|PDB:2ZXJ}.
FT   HELIX       144    146       {ECO:0000244|PDB:2ZXJ}.
FT   STRAND      148    151       {ECO:0000244|PDB:2ZXJ}.
FT   STRAND      154    156       {ECO:0000244|PDB:2ZXJ}.
FT   HELIX       160    170       {ECO:0000244|PDB:2ZXJ}.
FT   TURN        171    174       {ECO:0000244|PDB:2ZXJ}.
FT   HELIX       179    187       {ECO:0000244|PDB:2ZXJ}.
FT   HELIX       196    209       {ECO:0000244|PDB:2ZXJ}.
FT   STRAND      217    222       {ECO:0000244|PDB:2ZXJ}.
FT   TURN        223    225       {ECO:0000244|PDB:2ZXJ}.
FT   STRAND      226    229       {ECO:0000244|PDB:2ZXJ}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT       153    229       ipfam:Trans_reg_C [T]
FT   MYHIT       153    229       ismart:Trans_reg_C [T]
FT   MYHIT         5    114       ipfam:Response_reg [T]
FT   MYHIT         4    117       iprf:RESPONSE_REGULATORY [T]
FT   MYHIT         3    113       ismart:REC [T]
FT   MYHIT       132    231       iprf:OMPR_PHOB [T]
SQ   SEQUENCE   233 AA;  27192 MW;  BFA71D9C2464F600 CRC64;
     MARKVVVVDD EKPIADILEF NLKKEGYDVY CAYDGNDAVD LIYEEEPDIV LLDIMLPGRD
     GMEVCREVRK KYEMPIIMLT AKDSEIDKVL GLELGADDYV TKPFSTRELI ARVKANLRRH
     YSQPAQDTGN VTNEITIKDI VIYPDAYSIK KRGEDIELTH REFELFHYLS KHMGQVMTRE
     HLLQTVWGYD YFGDVRTVDV TIRRLREKIE DDPSHPEYIV TRRGVGYFLQ QHE
//