Legends: 1, 4-aspartylphosphate. {ECO:0000255|PROSITE-ProRule:PRU00169}; 2, Response regulatory. {ECO:0000255|PROSITE-ProRule:PRU00169}; 3, DNA_BIND OmpR/PhoB-type. {ECO:0000255|PROSITE- ProRule:PRU01091}; 4, STRAND {ECO:0000244|PDB:2ZXJ}; 5, HELIX {ECO:0000244|PDB:2ZXJ}; 6, TURN {ECO:0000244|PDB:2ZXJ}.
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ID WALR_STAAU Reviewed; 233 AA.
AC Q9RDT5;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 04-NOV-2008, sequence version 2.
DT 18-JAN-2017, entry version 93.
DE RecName: Full=Transcriptional regulatory protein WalR {ECO:0000305};
GN Name=walR; Synonyms=vicR, yycF;
OS Staphylococcus aureus.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12379689; DOI=10.1128/IAI.70.11.6121-6128.2002;
RA Wagner C., de Saizieu A., Schoenfeld H.-J., Kamber M., Lange R.,
RA Thompson C.J., Page M.G.;
RT "Genetic analysis and functional characterization of the Streptococcus
RT pneumoniae vic operon.";
RL Infect. Immun. 70:6121-6128(2002).
RN [2]
RP PHOSPHORYLATION.
RC STRAIN=WCUH29 / NCIMB 40771;
RX PubMed=12867749; DOI=10.1159/000071077;
RA Clausen V.A., Bae W., Throup J., Burnham M.K.R., Rosenberg M.,
RA Wallis N.G.;
RT "Biochemical characterization of the first essential two-component
RT signal transduction system from Staphylococcus aureus and
RT Streptococcus pneumoniae.";
RL J. Mol. Microbiol. Biotechnol. 5:252-260(2003).
RN [3]
RP OVEREXPRESSION.
RC STRAIN=SA137/93A;
RX PubMed=17418637; DOI=10.1016/j.ijmm.2007.02.002;
RA Jansen A., Tuerck M., Szekat C., Nagel M., Clever I., Bierbaum G.;
RT "Role of insertion elements and yycFG in the development of decreased
RT susceptibility to vancomycin in Staphylococcus aureus.";
RL Int. J. Med. Microbiol. 297:205-215(2007).
CC -!- FUNCTION: Member of the two-component regulatory system WalK/WalR
CC that regulates genes involved in autolysis, biofilm formation and
CC cell wall metabolism. {ECO:0000250|UniProtKB:Q2G2U6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by WalK. {ECO:0000269|PubMed:12867749}.
CC -!- MISCELLANEOUS: Overexpression of walK/walR reduces susceptibility
CC against vancomycin. {ECO:0000269|PubMed:17418637}.
CC -!- SIMILARITY: Contains 1 OmpR/PhoB-type DNA-binding domain.
CC {ECO:0000255|PROSITE-ProRule:PRU01091}.
CC -!- SIMILARITY: Contains 1 response regulatory domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00169}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB65399.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR EMBL; AJ012052; CAB65399.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_000101976.1; NZ_MLQK01000006.1.
DR PDB; 2ZXJ; X-ray; 1.87 A; A/B=123-233.
DR PDBsum; 2ZXJ; -.
DR ProteinModelPortal; Q9RDT5; -.
DR SMR; Q9RDT5; -.
DR STRING; 93062.SACOL0019; -.
DR GeneID; 28379619; -.
DR eggNOG; ENOG4105CK6; Bacteria.
DR eggNOG; COG0745; LUCA.
DR EvolutionaryTrace; Q9RDT5; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR011991; WHTH_DNA-bd_dom.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Cytoplasm; DNA-binding; Phosphoprotein;
KW Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1 233 Transcriptional regulatory protein WalR.
FT /FTId=PRO_0000353031.
FT DOMAIN 4 117 Response regulatory.
FT {ECO:0000255|PROSITE-ProRule:PRU00169}.
FT DNA_BIND 132 231 OmpR/PhoB-type. {ECO:0000255|PROSITE-
FT ProRule:PRU01091}.
FT MOD_RES 53 53 4-aspartylphosphate.
FT {ECO:0000255|PROSITE-ProRule:PRU00169}.
FT STRAND 135 137 {ECO:0000244|PDB:2ZXJ}.
FT STRAND 140 143 {ECO:0000244|PDB:2ZXJ}.
FT HELIX 144 146 {ECO:0000244|PDB:2ZXJ}.
FT STRAND 148 151 {ECO:0000244|PDB:2ZXJ}.
FT STRAND 154 156 {ECO:0000244|PDB:2ZXJ}.
FT HELIX 160 170 {ECO:0000244|PDB:2ZXJ}.
FT TURN 171 174 {ECO:0000244|PDB:2ZXJ}.
FT HELIX 179 187 {ECO:0000244|PDB:2ZXJ}.
FT HELIX 196 209 {ECO:0000244|PDB:2ZXJ}.
FT STRAND 217 222 {ECO:0000244|PDB:2ZXJ}.
FT TURN 223 225 {ECO:0000244|PDB:2ZXJ}.
FT STRAND 226 229 {ECO:0000244|PDB:2ZXJ}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 4 117 iprf:RESPONSE_REGULATORY [T]
FT MYHIT 5 114 ipfam:Response_reg [T]
FT MYHIT 132 231 iprf:OMPR_PHOB [T]
FT MYHIT 3 113 ismart:REC [T]
FT MYHIT 153 229 ipfam:Trans_reg_C [T]
FT MYHIT 153 229 ismart:Trans_reg_C [T]
SQ SEQUENCE 233 AA; 27192 MW; BFA71D9C2464F600 CRC64;
MARKVVVVDD EKPIADILEF NLKKEGYDVY CAYDGNDAVD LIYEEEPDIV LLDIMLPGRD
GMEVCREVRK KYEMPIIMLT AKDSEIDKVL GLELGADDYV TKPFSTRELI ARVKANLRRH
YSQPAQDTGN VTNEITIKDI VIYPDAYSIK KRGEDIELTH REFELFHYLS KHMGQVMTRE
HLLQTVWGYD YFGDVRTVDV TIRRLREKIE DDPSHPEYIV TRRGVGYFLQ QHE
//
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