ID WALR_STAA8 Reviewed; 233 AA.
AC Q2G2U6; Q9XCM7;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 02-NOV-2016, entry version 79.
DE RecName: Full=Transcriptional regulatory protein WalR {ECO:0000305};
GN Name=walR {ECO:0000303|PubMed:17827301}; Synonyms=yycF;
GN OrderedLocusNames=SAOUHSC_00020;
OS Staphylococcus aureus (strain NCTC 8325).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND MUTAGENESIS OF
RP GLU-63.
RX PubMed=10368139;
RA Martin P.K., Li T., Sun D., Biek D.P., Schmid M.B.;
RT "Role in cell permeability of an essential two-component system in
RT Staphylococcus aureus.";
RL J. Bacteriol. 181:3666-3673(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W.,
RA Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press,
RL Washington D.C. (2006).
RN [3]
RP FUNCTION IN REGULATING ISAA, LYTM AND SSAA, DNA-BINDING, AND
RP PHOSPHORYLATION.
RX PubMed=14762013; DOI=10.1128/JB.186.4.1175-1181.2004;
RA Dubrac S., Msadek T.;
RT "Identification of genes controlled by the essential YycG/YycF two-
RT component system of Staphylococcus aureus.";
RL J. Bacteriol. 186:1175-1181(2004).
RN [4]
RP FUNCTION IN REGULATING CELL WALL METABOLISM, BIOFILM FORMATION, AND
RP AUTOLYSIS.
RX PubMed=17827301; DOI=10.1128/JB.00645-07;
RA Dubrac S., Boneca I.G., Poupel O., Msadek T.;
RT "New insights into the WalK/WalR (YycG/YycF) essential signal
RT transduction pathway reveal a major role in controlling cell wall
RT metabolism and biofilm formation in Staphylococcus aureus.";
RL J. Bacteriol. 189:8257-8269(2007).
CC -!- FUNCTION: Member of the two-component regulatory system WalK/WalR
CC that regulates genes involved in autolysis, biofilm formation and
CC cell wall metabolism. Positively controls the cell wall-hydrolytic
CC activity through regulation of atlA and lytM, as well as induces
CC transcription of isaA, sceD, ssaA, and four ssaA-related genes.
CC Binds directly to the lytM, ssaA and isaA promoter regions in
CC vitro. {ECO:0000269|PubMed:10368139, ECO:0000269|PubMed:14762013,
CC ECO:0000269|PubMed:17827301}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by WalK. {ECO:0000269|PubMed:14762013}.
CC -!- SIMILARITY: Contains 1 OmpR/PhoB-type DNA-binding domain.
CC {ECO:0000255|PROSITE-ProRule:PRU01091}.
CC -!- SIMILARITY: Contains 1 response regulatory domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00169}.
DR EMBL; AF136709; AAD40237.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD29209.1; -; Genomic_DNA.
DR RefSeq; WP_000101976.1; NC_007795.1.
DR RefSeq; YP_498626.1; NC_007795.1.
DR ProteinModelPortal; Q2G2U6; -.
DR SMR; Q2G2U6; -.
DR STRING; 93061.SAOUHSC_00020; -.
DR EnsemblBacteria; ABD29209; ABD29209; SAOUHSC_00020.
DR GeneID; 28379619; -.
DR GeneID; 3919191; -.
DR KEGG; sao:SAOUHSC_00020; -.
DR PATRIC; 19577684; VBIStaAur99865_0018.
DR eggNOG; ENOG4105CK6; Bacteria.
DR eggNOG; COG0745; LUCA.
DR HOGENOM; HOG000034819; -.
DR KO; K07668; -.
DR OMA; FHYLANH; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR011991; WHTH_DNA-bd_dom.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Complete proteome; Cytoplasm; DNA-binding; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1 233 Transcriptional regulatory protein WalR.
FT /FTId=PRO_0000353042.
FT DOMAIN 4 117 Response regulatory.
FT {ECO:0000255|PROSITE-ProRule:PRU00169}.
FT DNA_BIND 132 231 OmpR/PhoB-type. {ECO:0000255|PROSITE-
FT ProRule:PRU01091}.
FT MOD_RES 53 53 4-aspartylphosphate.
FT {ECO:0000255|PROSITE-ProRule:PRU00169}.
FT MUTAGEN 63 63 E->K: Fails to form colonies on rich and
FT defined media at temperatures above 40
FT degrees Celsius. Increased susceptibility
FT to linoleic acid and oleic acid.
FT Increased susceptibility to macrolide and
FT lincosamide antibiotics.
FT {ECO:0000269|PubMed:10368139}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 153 229 ipfam:Trans_reg_C [T]
FT MYHIT 5 114 ipfam:Response_reg [T]
FT MYHIT 153 229 ismart:Trans_reg_C [T]
FT MYHIT 4 117 iprf:RESPONSE_REGULATORY [T]
FT MYHIT 3 113 ismart:REC [T]
FT MYHIT 132 231 iprf:OMPR_PHOB [T]
SQ SEQUENCE 233 AA; 27192 MW; BFA71D9C2464F600 CRC64;
MARKVVVVDD EKPIADILEF NLKKEGYDVY CAYDGNDAVD LIYEEEPDIV LLDIMLPGRD
GMEVCREVRK KYEMPIIMLT AKDSEIDKVL GLELGADDYV TKPFSTRELI ARVKANLRRH
YSQPAQDTGN VTNEITIKDI VIYPDAYSIK KRGEDIELTH REFELFHYLS KHMGQVMTRE
HLLQTVWGYD YFGDVRTVDV TIRRLREKIE DDPSHPEYIV TRRGVGYFLQ QHE
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