Entry sw:VILB_DICDI

ID   VILB_DICDI              Reviewed;         959 AA.
AC   P36418; Q551N4;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   30-NOV-2016, entry version 102.
DE   RecName: Full=Protovillin;
DE   AltName: Full=100 kDa actin-binding protein;
GN   Name=vilB; ORFNames=DDB_G0276453;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 569-586 AND
RP   843-853.
RC   STRAIN=AX2, and AX3;
RX   PubMed=8344435; DOI=10.1016/0014-5793(93)80968-Z;
RA   Hofmann A., Noegel A.A., Bomblies L., Lottspeich F., Schleicher M.;
RT   "The 100 kDa F-actin capping protein of Dictyostelium amoebae is a
RT   villin prototype ('protovillin').";
RL   FEBS Lett. 328:71-76(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A.,
RA   Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G.,
RA   Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A.,
RA   Platzer M., Rosenthal A., Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Caps actin filaments but displays neither severing nor
CC       cross-linking nor nucleating activities. Protovillin seems to be a
CC       villin precursor with only archaic capping activity. It lacks
CC       essential changes in the sequence to allow bundling of actin
CC       filaments and consequently the appearance of microvilli.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC   -!- DEVELOPMENTAL STAGE: Present in vegetative and developing cells.
CC   -!- SIMILARITY: Belongs to the villin/gelsolin family. {ECO:0000305}.
CC   -!- SIMILARITY: Contains 6 gelsolin-like repeats. {ECO:0000305}.
CC   -!- SIMILARITY: Contains 1 HP (headpiece) domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00595}.
DR   EMBL; X74387; CAA52410.1; -; Genomic_DNA.
DR   EMBL; AAFI02000015; EAL69180.1; -; Genomic_DNA.
DR   PIR; S35061; S35061.
DR   RefSeq; XP_643085.1; XM_637993.1.
DR   ProteinModelPortal; P36418; -.
DR   STRING; 44689.DDB0185072; -.
DR   PaxDb; P36418; -.
DR   EnsemblProtists; EAL69180; EAL69180; DDB_G0276453.
DR   GeneID; 8620489; -.
DR   KEGG; ddi:DDB_G0276453; -.
DR   dictyBase; DDB_G0276453; vilB.
DR   eggNOG; KOG0443; Eukaryota.
DR   eggNOG; ENOG410XR0A; LUCA.
DR   InParanoid; P36418; -.
DR   KO; K05761; -.
DR   OMA; CFSHINE; -.
DR   PhylomeDB; P36418; -.
DR   PRO; PR:P36418; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   Proteomes; UP000002195; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0003785; F:actin monomer binding; IDA:dictyBase.
DR   GO; GO:0051016; P:barbed-end actin filament capping; IDA:dictyBase.
DR   GO; GO:0007010; P:cytoskeleton organization; IEA:InterPro.
DR   Gene3D; 1.10.950.10; -; 1.
DR   Gene3D; 3.40.20.10; -; 6.
DR   InterPro; IPR029006; ADF-H/Gelsolin-like_dom.
DR   InterPro; IPR007123; Gelsolin-like_dom.
DR   InterPro; IPR007122; Villin/Gelsolin.
DR   InterPro; IPR003128; Villin_headpiece.
DR   PANTHER; PTHR11977; PTHR11977; 2.
DR   Pfam; PF00626; Gelsolin; 5.
DR   Pfam; PF02209; VHP; 1.
DR   PRINTS; PR00597; GELSOLIN.
DR   SMART; SM00262; GEL; 6.
DR   SMART; SM00153; VHP; 1.
DR   SUPFAM; SSF47050; SSF47050; 1.
DR   PROSITE; PS51089; HP; 1.
PE   1: Evidence at protein level;
KW   Actin capping; Actin-binding; Complete proteome; Cytoplasm;
KW   Cytoskeleton; Direct protein sequencing; Reference proteome; Repeat.
FT   CHAIN         1    959       Protovillin.
FT                                /FTId=PRO_0000218731.
FT   REPEAT       64    116       Gelsolin-like 1.
FT   REPEAT      204    244       Gelsolin-like 2.
FT   REPEAT      309    366       Gelsolin-like 3.
FT   REPEAT      479    529       Gelsolin-like 4.
FT   REPEAT      603    647       Gelsolin-like 5.
FT   REPEAT      713    754       Gelsolin-like 6.
FT   REPEAT      840    849       1.
FT   REPEAT      851    860       2.
FT   DOMAIN      895    959       HP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00595}.
FT   REGION        1     53       Tail.
FT   REGION       54    832       Core.
FT   REGION      840    860       2 X 10 AA repeats of T-P-K-P-I-T-T-P-T-V.
FT   COMPBIAS    833    880       Pro/Thr-rich (neck).
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT       469    567       ismart:GEL [T]
FT   MYHIT       924    959       ismart:VHP [T]
FT   MYHIT       592    679       ismart:GEL [T]
FT   MYHIT        53    168       ismart:GEL [T]
FT   MYHIT       333    399       ipfam:Gelsolin [T]
FT   MYHIT       480    561       ipfam:Gelsolin [T]
FT   MYHIT       312    406       ismart:GEL [T]
FT   MYHIT        64    162       ipfam:Gelsolin [T]
FT   MYHIT       895    959       iprf:HP [T]
FT   MYHIT       702    795       ismart:GEL [T]
FT   MYHIT       191    285       ismart:GEL [T]
FT   MYHIT       714    788       ipfam:Gelsolin [T]
FT   MYHIT       203    279       ipfam:Gelsolin [T]
FT   MYHIT       924    959       ipfam:VHP [T]
SQ   SEQUENCE   959 AA;  109462 MW;  0A4716EE16E52801 CRC64;
     MEPPLELPTQ RKRVIPSKFG ILKRNAEIEA EKNRENLQQS SCFSHINEIG KEIGLEIWKI
     IDDSTIQKVP KVNHSTFETN KSYLLLMGQF YDGNMNIKTY NIHFWIGELL INSQETINFC
     NDRIEELERI IKYNQKQFDS EQFYPEPILY REFQGKEGDI FMSYFKSYGG PRYVAPLKLT
     SASAAIATAA KQYKLFHLKG RRNIRVKQVD ISSKSLNSGD VFVLDCEDFI YQWNGSESSR
     LEKGKGLDLT IRLRDEKSAK AKIIVMDEND TDKDHPEFWK RLGGCKDDVQ KAEQGGDDFA
     YEKKSVEQIK LYQVENLNYE VHLHLIDPIG DVYSTTQLNA EFCYILDCET ELYVWLGKAS
     ANDQRTVAMA NAMDLLHEDN RPSWTPIIKM TQGSENTLFK DKFKKGSWGE YVNDNFEKKP
     ITGKGVAAKA VQEKINVDAL HNPEKYQLSK EERKSTIPTL HHVDDKHRGE LKIWHVRNRN
     KFEISQSEFG LFYNQSCYLV LFTLFAADGS NNSILYYWQG RFSSSEDKGA AALLAKDVGK
     ELHRSCIHVR TVQNKEPNHF LEHFQGRMVV FKGSRPNATT EVSLENLSSS LQGLYHVRGT
     EPINIHSIQV EKAISSLDSN DSFILVNFKN TISYIWVGKY SDEKEAALQI SSNVFTGYNF
     QLIDEGDETS EFWESLETNS SLSLLKDYYT QLRTVEQEKK TRLFQCSNNS GVFKVFEIHD
     FSQDDLDSDD VMILDNQKQI FVWVGKESSD TEKLMANETA LEYIMNAPTH RRDDPIFTIQ
     DGFEPHEFTF NFHAWQVNKT QQDSYKSKLS AILGSNNSGP ASPIMLPTSG VTLKPTTAAT
     PKPITTPTVT TPKPITTPTV ATLKTVTPAV TLKPTTVTTP SKVATTTNTS TPSPTTITTF
     YPLSVLKQKT NLPNDIDKSC LHLYLSDEEF LSTFKMTKEI FQKTPAWKTK QLRVDNGLF
//