Legends: 1, Helicase ATP-binding 1. {ECO:0000255|PROSITE-ProRule:PRU00541}; 2, Helicase C-terminal. {ECO:0000255|PROSITE-ProRule:PRU00542}; 3, SEC63 1. {ECO:0000255}; 4, Helicase ATP-binding 2. {ECO:0000255|PROSITE-ProRule:PRU00541}; 5, SEC63 2. {ECO:0000255}; 6, NP_BIND ATP. {ECO:0000255|PROSITE- ProRule:PRU00541}; 7, MOTIF DEAH box. {ECO:0000255|PROSITE- ProRule:PRU00541}; 8, COMPBIAS Poly-Glu. {ECO:0000255}; 9, ipfam:DEAD [T]; 10, ismart:HELICc [T]; 11, ismart:DEXDc [T]; 12, ismart:AAA [T]; 13, iprf:HELICASE_ATP_BIND_1 [T]; 14, iprf:HELICASE_CTER [T]; 15, ipfam:Helicase_C [T].
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ID U520_CAEEL Reviewed; 2145 AA.
AC Q9U2G0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 30-NOV-2016, entry version 119.
DE RecName: Full=Putative U5 small nuclear ribonucleoprotein 200 kDa helicase;
DE EC=3.6.4.13 {ECO:0000250|UniProtKB:O75643};
GN Name=snrp-200 {ECO:0000312|WormBase:Y46G5A.4};
GN ORFNames=Y46G5A.4 {ECO:0000312|WormBase:Y46G5A.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for
RT investigating biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Putative RNA helicase involved in the second step of RNA
CC splicing. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC {ECO:0000250|UniProtKB:O75643}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75643}.
CC -!- DOMAIN: Contains two helicase domains. The N-terminal helicase
CC domain has catalytic activity by itself, contrary to the C-
CC terminal helicase domain that may have a regulatory role and
CC enhance the activity of the first helicase domain.
CC {ECO:0000250|UniProtKB:O75643}.
CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC {ECO:0000305}.
CC -!- SIMILARITY: Contains 2 helicase ATP-binding domains.
CC {ECO:0000255|PROSITE-ProRule:PRU00541}.
CC -!- SIMILARITY: Contains 1 helicase C-terminal domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00542}.
CC -!- SIMILARITY: Contains 2 SEC63 domains. {ECO:0000305}.
DR EMBL; AL110485; CAB60351.1; -; Genomic_DNA.
DR RefSeq; NP_496710.1; NM_064309.3.
DR UniGene; Cel.14062; -.
DR ProteinModelPortal; Q9U2G0; -.
DR SMR; Q9U2G0; -.
DR BioGrid; 40201; 2.
DR IntAct; Q9U2G0; 1.
DR MINT; MINT-1062451; -.
DR STRING; 6239.Y46G5A.4; -.
DR EPD; Q9U2G0; -.
DR PaxDb; Q9U2G0; -.
DR PeptideAtlas; Q9U2G0; -.
DR PRIDE; Q9U2G0; -.
DR EnsemblMetazoa; Y46G5A.4; Y46G5A.4; WBGene00012896.
DR GeneID; 174901; -.
DR KEGG; cel:CELE_Y46G5A.4; -.
DR UCSC; Y46G5A.4; c. elegans.
DR CTD; 174901; -.
DR WormBase; Y46G5A.4; CE21971; WBGene00012896; snrp-200.
DR eggNOG; KOG0952; Eukaryota.
DR eggNOG; COG1204; LUCA.
DR GeneTree; ENSGT00640000091272; -.
DR HOGENOM; HOG000157749; -.
DR InParanoid; Q9U2G0; -.
DR KO; K12854; -.
DR OMA; KKMENWW; -.
DR OrthoDB; EOG091G0HE5; -.
DR PhylomeDB; Q9U2G0; -.
DR Reactome; R-CEL-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-CEL-72165; mRNA Splicing - Minor Pathway.
DR PRO; PR:Q9U2G0; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00012896; -.
DR GO; GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR Gene3D; 2.60.40.150; -; 2.
DR Gene3D; 3.40.50.300; -; 5.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004179; Sec63-dom.
DR InterPro; IPR011991; WHTH_DNA-bd_dom.
DR Pfam; PF00270; DEAD; 2.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF02889; Sec63; 2.
DR SMART; SM00382; AAA; 2.
DR SMART; SM00487; DEXDc; 2.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00973; Sec63; 2.
DR SUPFAM; SSF46785; SSF46785; 2.
DR SUPFAM; SSF52540; SSF52540; 4.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 2.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing;
KW mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome;
KW Repeat; Spliceosome.
FT CHAIN 1 2145 Putative U5 small nuclear
FT ribonucleoprotein 200 kDa helicase.
FT /FTId=PRO_0000102088.
FT DOMAIN 484 667 Helicase ATP-binding 1.
FT {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT DOMAIN 677 894 Helicase C-terminal.
FT {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT DOMAIN 975 1278 SEC63 1. {ECO:0000255}.
FT DOMAIN 1331 1506 Helicase ATP-binding 2.
FT {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT DOMAIN 1812 2124 SEC63 2. {ECO:0000255}.
FT NP_BIND 497 504 ATP. {ECO:0000255|PROSITE-
FT ProRule:PRU00541}.
FT NP_BIND 1344 1351 ATP. {ECO:0000255|PROSITE-
FT ProRule:PRU00541}.
FT MOTIF 609 612 DEAH box. {ECO:0000255|PROSITE-
FT ProRule:PRU00541}.
FT MOTIF 1448 1451 DEAH box. {ECO:0000255|PROSITE-
FT ProRule:PRU00541}.
FT COMPBIAS 221 228 Poly-Glu. {ECO:0000255}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 1324 1489 ipfam:DEAD [T]
FT MYHIT 761 853 ismart:HELICc [T]
FT MYHIT 471 681 ismart:DEXDc [T]
FT MYHIT 1336 1489 ismart:AAA [T]
FT MYHIT 489 805 ismart:AAA [T]
FT MYHIT 477 652 ipfam:DEAD [T]
FT MYHIT 1812 2124 ipfam:Sec63 [T]
FT MYHIT 1331 1506 iprf:HELICASE_ATP_BIND_1 [T]
FT MYHIT 1318 1522 ismart:DEXDc [T]
FT MYHIT 974 1279 ismart:Sec63 [T]
FT MYHIT 484 667 iprf:HELICASE_ATP_BIND_1 [T]
FT MYHIT 1811 2126 ismart:Sec63 [T]
FT MYHIT 975 1278 ipfam:Sec63 [T]
FT MYHIT 677 894 iprf:HELICASE_CTER [T]
FT MYHIT 708 851 ipfam:Helicase_C [T]
SQ SEQUENCE 2145 AA; 243831 MW; 2C1EE0F69583891F CRC64;
MADELARIQQ YEYRQNSNLV LSVDYNLTDR RGREEPTGEV LPITDKEMRK MKMGDRAIKG
KAPVQDQKKK RKKKDDEKAQ QFGRNVLVDN NELMGAYKPR TQETKQTYEV ILSFILDALG
DVPREVLCGA ADEVLLTLKN DKFRDKEKKK EVEALLGPLT DDRIAVLINL SKKISDFSIE
EENKPEGDGD IYENEGVNVQ FDSDEEEDDG GMVNEIKGDS EEESEEEEGV DTDYTATLKG
DGHLTEDEQK ARGILHPRDI DAHWIQRSLA KYFKDPLIAQ QKQTEVIGIL KNAADDRDAE
NQLVLLLGFD QFEFIKCLRQ NRLMILYCTL LRQANEKERL QIEDDMRSRP ELHPILALLQ
ETDEGSVVQV EKSKRDAEKS KKAATAANEA ISAGQWQAGR KMLDLNDLTF SQGSHLMSNK
RCELPDGSYR RQKKSYEEIH VPALKPRPFA EGEKLVSVSE LPKWAQPAFD GYKSLNRIQS
RLCDSALRSK EHLLLCAPTG AGKTNVALLT MLQEIGNHLA EDGSVKLDEF KIVYIAPMKS
LVQEMVGSFS KRLAPFGITV GEMTGDAQMS KEQFMATQVI VCTPEKYDVV TRKGGERAYN
QMVRLLIIDE IHLLHDDRGP VLESIVVRTI RQMEQNHDEC RLVGLSATLP NYQDVATFLR
VKPEHLHFFD NSYRPVPLEQ QYIGVTEKKA LKRFQAMNEV VYDKIMEHAG KSQVLVFVHS
RKETAKTAKA IRDACLEKDT LSAFMREGSA STEILRTEAE QAKNLDLKDL LPYGFAIHHA
GMNRVDRTLV EDLFADRHIQ VLFSTATLAW GVNLPAHTVI IKGTQIYNPE KGRWTELGAL
DIMQMLGRAG RPQYDDRGEG ILITNHSELQ YYLSLMNQQL PVESQMVSRL TDMLNAEVVL
GTVSSVSEAT NWLGYTFLFV RMLKNPTLYG ITHEQARADP LLEQRRADLI HTACVLLDKA
GLIKYDKRSG IIQATELGRI ASHFYCTYES MQTYNKLLVE TCSDIDLFRI FSMSSEFKLL
SVRDEEKLEL QKMAEHAPIP IKENLDEASA KTNVLLQAYI SQLKLEGFAL QADMVFVAQS
AGRLFRALFE IVLWRGWAGL AQKVLTLCKM VTQRQWGSLN PLHQFKKIPS EVVRSIDKKN
YSFDRLYDLD QHQLGDLIKM PKMGKPLFKF IRQFPKLEMT TLIQPITRTT MRIELTITPD
FKWDEKVHGS AEGFWIFIED TDGEKILHHE FFLLKQKFCS DEHVVKMIVP MFDPMPPLYY
VRIVSDRWIG AETVLPISFR HLILPEKYPP PTELLDLQPL PISAVTNKEF QTVFAESGFK
VFNPIQTQVF RTVFESNENV IVCAPNGSGK TAIAELAVLR HFENTPEAKA VYITPMEDMA
TKVYADWKRR LEPAIGHTIV LLTGEQTMDL KLAQRGQLII STPERWDNIS RRWKQRKSVQ
NVKLFIADDL HMIGASNGAV FEVVCSRTRY ISSQLESAVR VVALSSSLTN ARDLGMWLGC
SASATFNFMP STRPVPLDLE IKSFNLSHNA SRFAAMERPV YQAICRHAGK LEPKPALVFV
PVRRQTRPVA VALLTMALAD GAPKRFLRLA EHDDTFQALL ADIEDESLRE SVSCGVGFLH
EGTAPKDVHI VQQLFESNAI QVCVVPRGMC YQIEMSAYLV VVMDTQFYNG KYHVYEDYPI
ADMLHMVGLA NRPILDSDAK CVVMCQTSKR AYYKKFLCDP LPVESHLDHC LHDHFNAEIV
TKTIENKQDA IDYLTWTLLY RRMTQNPNYY NLQGTTHRHL SDALSELVEL TLKDLENSKC
IAVKDEMDTV SLNLGMIASY YYISYQTIEL FSMSLKEKTK TRALIEIISA SSEFGNVPMR
HKEDVILRQL AERLPGQLKN QKFTDPHVKV NLLIHAHLSR VKLTAELNKD TELIVLRACR
LVQACVDVLS SNGWLSPAIH AMELSQMLTQ AMYSNEPYLK QLPHCSAALL ERAKAKEVTS
VFELLELEND DRSDILQMEG AELADVARFC NHYPSIEVAT ELENDVVTSN DNLMLAVSLE
RDNDIDGLAP PVVAPLFPQK RKEEGWWLVI GDSESNALLT IKRLVINEKS SVQLDFAAPR
PGHHKFKLFF ISDSYLGADQ EFDVAFKVEE PGRSNRKRKH EKEED
//
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