ID TRUB_YERE8 Reviewed; 329 AA.
AC A1JIX1;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 02-NOV-2016, entry version 72.
DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=YE0436;
OS Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 /
OS 8081).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=393305;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 13174 / 8081;
RX PubMed=17173484; DOI=10.1371/journal.pgen.0020206;
RA Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L.,
RA Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T.,
RA Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S.,
RA Sanders M., Whitehead S., Quail M.A., Dougan G., Parkhill J.,
RA Prentice M.B.;
RT "The complete genome sequence and comparative genome analysis of the
RT high pathogenicity Yersinia enterocolitica strain 8081.";
RL PLoS Genet. 2:2039-2051(2006).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC 55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC Rule:MF_01080}.
CC -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55).
CC {ECO:0000255|HAMAP-Rule:MF_01080}.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
DR EMBL; AM286415; CAL10562.1; -; Genomic_DNA.
DR RefSeq; WP_011815446.1; NC_008800.1.
DR RefSeq; YP_001004806.1; NC_008800.1.
DR ProteinModelPortal; A1JIX1; -.
DR STRING; 393305.YE0436; -.
DR EnsemblBacteria; CAL10562; CAL10562; YE0436.
DR GeneID; 4715811; -.
DR KEGG; yen:YE0436; -.
DR PATRIC; 18560282; VBIYerEnt11519_0532.
DR eggNOG; ENOG4105D0T; Bacteria.
DR eggNOG; COG0130; LUCA.
DR HOGENOM; HOG000231223; -.
DR KO; K03177; -.
DR OMA; YELQFIR; -.
DR OrthoDB; POG091H02G9; -.
DR Proteomes; UP000000642; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-HAMAP.
DR Gene3D; 2.30.130.10; -; 1.
DR HAMAP; MF_01080; TruB_bact; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR015947; PUA-like_domain.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C.
DR InterPro; IPR032819; TruB_C.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF09157; TruB-C_2; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isomerase; tRNA processing.
FT CHAIN 1 329 tRNA pseudouridine synthase B.
FT /FTId=PRO_1000084715.
FT ACT_SITE 48 48 Nucleophile. {ECO:0000255|HAMAP-
FT Rule:MF_01080}.
FT BINDING 43 43 Substrate. {ECO:0000255|HAMAP-
FT Rule:MF_01080}.
FT BINDING 76 76 Substrate. {ECO:0000255|HAMAP-
FT Rule:MF_01080}.
FT BINDING 179 179 Substrate. {ECO:0000255|HAMAP-
FT Rule:MF_01080}.
FT BINDING 200 200 Substrate. {ECO:0000255|HAMAP-
FT Rule:MF_01080}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 33 180 ipfam:TruB_N [T]
FT MYHIT 252 309 ipfam:TruB-C_2 [T]
FT MYHIT 11 309 ihamap:TruB_bact [T]
FT MYHIT 181 248 ipfam:TruB_C_2 [T]
SQ SEQUENCE 329 AA; 35874 MW; FDA7D3AE227A3324 CRC64;
MGRPRRRGRD INGVLLLDKP LGLSSNDVLQ KVKRLFSANR AGHTGALDPL ATGMLPICLG
EATKFSQFLL DSDKRYRVVA RLGQRTDTSD AEGALISERE VNVTQAQMDA ALDSFRGSSQ
QVPSMYSALK HQGKPLYEYA RQGIEVEREA RSITVYELLF IRWEGNDLEL EIHCSKGTYI
RTIIDDLGEL LGCGAHVSYL RRLQVATYPS DRMVTLEQLT AIVETAQAEE RSPNAELDAL
LLPMDSAVLN FPEVNLLPAV AAYVKQGQPV HVAGTPGEGM VRITEGEERN FIGIGTIAED
GRVAPKRLVV EHVDGANPVV AGVAAGNTP
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