Legends: 1, N-linked (GlcNAc...). {ECO:0000255}; 2, CONFLICT Y -> N (in Ref. 1; AAF64509). {ECO:0000305}; 3, CONFLICT V -> A (in Ref. 1; AAF64509). {ECO:0000305}; 4, CONFLICT I -> F (in Ref. 1; AAF64509). {ECO:0000305}; 5, CONFLICT T -> S (in Ref. 1; AAF64509). {ECO:0000305}; 6, CONFLICT T -> I (in Ref. 1; AAF64509). {ECO:0000305}; 7, CONFLICT C -> H (in Ref. 1; AAF64509). {ECO:0000305}; 8, CONFLICT L -> V (in Ref. 1; AAF64509). {ECO:0000305}; 9, TOPO_DOM Extracellular. {ECO:0000255}; 10, TRANSMEM Helical; Name=1. {ECO:0000255}; 11, TOPO_DOM Cytoplasmic. {ECO:0000255}; 12, TRANSMEM Helical; Name=2. {ECO:0000255}; 13, TRANSMEM Helical; Name=3. {ECO:0000255}; 14, TRANSMEM Helical; Name=4. {ECO:0000255}; 15, TRANSMEM Helical; Name=5. {ECO:0000255}; 16, TRANSMEM Helical; Name=6. {ECO:0000255}; 17, TRANSMEM Helical; Name=7. {ECO:0000255}; 18, CONFLICT Missing (in Ref. 1). {ECO:0000305}; 19, CONFLICT EA -> KV (in Ref. 1; AAF64509). {ECO:0000305}; 20, CONFLICT GL -> RR (in Ref. 1; AAF64509). {ECO:0000305}; 21, CONFLICT LRSIGV -> FTEYRR (in Ref. 1; AAF64509). {ECO:0000305}.
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ID TR103_MOUSE Reviewed; 312 AA.
AC Q9JKA3; Q7M706;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 02-NOV-2016, entry version 98.
DE RecName: Full=Taste receptor type 2 member 103;
DE Short=T2R103;
DE AltName: Full=Taste receptor family B member 2;
DE Short=TRB2;
DE AltName: Full=Taste receptor type 2 member 10;
DE Short=T2R10;
GN Name=Tas2r103; Synonyms=Tas2r10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S.,
RA She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W.,
RA Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T.,
RA Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J.,
RA Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z.,
RA Lindblad-Toh K., Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of
RT the mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 36-277.
RC STRAIN=DBA/2J;
RX PubMed=10766242; DOI=10.1038/35007072;
RA Matsunami H., Montmayeur J.-P., Buck L.B.;
RT "A family of candidate taste receptors in human and mouse.";
RL Nature 404:601-604(2000).
RN [3]
RP IDENTIFICATION.
RX PubMed=12679530; DOI=10.1093/molbev/msg083;
RA Shi P., Zhang J., Yang H., Zhang Y.-P.;
RT "Adaptive diversification of bitter taste receptor genes in mammalian
RT evolution.";
RL Mol. Biol. Evol. 20:805-814(2003).
RN [4]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/S0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [5]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.R100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [6]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/S0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Gustducin-coupled receptor implicated in the perception
CC of bitter compounds in the oral cavity and the gastrointestinal
CC tract. Signals through PLCB2 and the calcium-regulated cation
CC channel TRPM5.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells
CC of the tongue and palate epithelium and exclusively in gustducin-
CC positive cells. Expressed in 15% taste bud cells in circumvallate
CC and foliate papillae but only in 2% in fungiform papillae.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a
CC single taste receptor cell.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
DR EMBL; AC152822; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF247732; AAF64509.1; -; Genomic_DNA.
DR EMBL; BK001091; DAA01230.1; -; Genomic_DNA.
DR CCDS; CCDS51933.1; -.
DR RefSeq; NP_444441.1; NM_053211.1.
DR UniGene; Mm.479865; -.
DR ProteinModelPortal; Q9JKA3; -.
DR STRING; 10090.ENSMUSP00000032317; -.
DR PhosphoSitePlus; Q9JKA3; -.
DR PaxDb; Q9JKA3; -.
DR PRIDE; Q9JKA3; -.
DR Ensembl; ENSMUST00000032317; ENSMUSP00000032317; ENSMUSG00000030196.
DR GeneID; 667992; -.
DR KEGG; mmu:667992; -.
DR UCSC; uc012eug.1; mouse.
DR CTD; 667992; -.
DR MGI; MGI:1890257; Tas2r103.
DR GeneTree; ENSGT00760000119096; -.
DR HOGENOM; HOG000234595; -.
DR HOVERGEN; HBG060698; -.
DR InParanoid; Q9JKA3; -.
DR KO; K08474; -.
DR OMA; RLLTISW; -.
DR OrthoDB; EOG091G0JBA; -.
DR PhylomeDB; Q9JKA3; -.
DR TreeFam; TF335891; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR PRO; PR:Q9JKA3; -.
DR Proteomes; UP000000589; Chromosome 6.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0033038; F:bitter taste receptor activity; IBA:GO_Central.
DR GO; GO:0004930; F:G-protein coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IBA:GO_Central.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR007960; T2R.
DR PANTHER; PTHR11394; PTHR11394; 1.
DR Pfam; PF05296; TAS2R; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Complete proteome; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Taste; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1 312 Taste receptor type 2 member 103.
FT /FTId=PRO_0000082243.
FT TOPO_DOM 1 6 Extracellular. {ECO:0000255}.
FT TRANSMEM 7 27 Helical; Name=1. {ECO:0000255}.
FT TOPO_DOM 28 61 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 62 82 Helical; Name=2. {ECO:0000255}.
FT TOPO_DOM 83 92 Extracellular. {ECO:0000255}.
FT TRANSMEM 93 113 Helical; Name=3. {ECO:0000255}.
FT TOPO_DOM 114 132 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 133 153 Helical; Name=4. {ECO:0000255}.
FT TOPO_DOM 154 185 Extracellular. {ECO:0000255}.
FT TRANSMEM 186 206 Helical; Name=5. {ECO:0000255}.
FT TOPO_DOM 207 229 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 230 250 Helical; Name=6. {ECO:0000255}.
FT TOPO_DOM 251 264 Extracellular. {ECO:0000255}.
FT TRANSMEM 265 285 Helical; Name=7. {ECO:0000255}.
FT TOPO_DOM 286 312 Cytoplasmic. {ECO:0000255}.
FT CARBOHYD 166 166 N-linked (GlcNAc...). {ECO:0000255}.
FT CONFLICT 52 52 Y -> N (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 72 75 Missing (in Ref. 1). {ECO:0000305}.
FT CONFLICT 125 125 V -> A (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 129 130 EA -> KV (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 146 146 I -> F (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 172 172 T -> S (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 177 178 GL -> RR (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 189 189 T -> I (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 213 213 C -> H (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 248 248 L -> V (in Ref. 1; AAF64509).
FT {ECO:0000305}.
FT CONFLICT 267 272 LRSIGV -> FTEYRR (in Ref. 1; AAF64509).
FT {ECO:0000305}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 11 300 ipfam:TAS2R [T]
FT MYHIT 27 241 iprf:G_PROTEIN_RECEP_F1_2 [T]
SQ SEQUENCE 312 AA; 35156 MW; 25972A2299781111 CRC64;
MVLTIRAILW VTLITIISLE FIIGILGNVF IALVNIIDWV KRGKISAVDK TYMALAISRT
AFLLSLITGF LVSLLDPALL GMRTMVRLLT ISWMVTNHFS VWFATCLSIF YFLKIANFSN
SIFLVLKWEA KKVVSVTLVV SVIILIMNII VINKFTDRLQ VNTLQNCSTS NTLKDYGLFL
FISTGFTLTP FAVSLTMFLL LIFSLWRHLK NMCHSATGSR DVSTVAHIKG LQTVVTFLLL
YTAFVMSLLS ESLNINIQHT NLLSHFLRSI GVAFPTGHSC VLILGNSKLR QASLSVILWL
RYKYKHIENW GP
//
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