ID TPPC4_HUMAN Reviewed; 219 AA.
AC Q9Y296; A8K3A5; B4DME1;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 30-NOV-2016, entry version 144.
DE RecName: Full=Trafficking protein particle complex subunit 4;
DE AltName: Full=Hematopoietic stem/progenitor cell protein 172;
DE AltName: Full=Synbindin;
DE AltName: Full=TRS23 homolog;
GN Name=TRAPPC4; Synonyms=SBDN; ORFNames=CGI-104, HSPC172, PTD009;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Zhu X., Zhao X.;
RT "Human unknown mRNA highly conserved among eukaryotes.";
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Pituitary tumor;
RA Peng Y., Song H., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G.,
RA Luo M., Chen J., Hu R.;
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Umbilical cord blood;
RX PubMed=11042152; DOI=10.1101/gr.140200;
RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G.,
RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W.,
RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
RT "Cloning and functional analysis of cDNAs with open reading frames for
RT 300 previously undefined genes expressed in CD34+ hematopoietic
RT stem/progenitor cells.";
RL Genome Res. 10:1546-1560(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F.,
RA Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E.,
RA FitzGerald M.G., Jaffe D.B., LaButti K., Nicol R., Park H.-S.,
RA Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W.,
RA Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S.,
RA Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP IDENTIFICATION IN TRAPP COMPLEX.
RX PubMed=11805826; DOI=10.1038/415141a;
RA Gavin A.-C., Boesche M., Krause R., Grandi P., Marzioch M., Bauer A.,
RA Schultz J., Rick J.M., Michon A.-M., Cruciat C.-M., Remor M.,
RA Hoefert C., Schelder M., Brajenovic M., Ruffner H., Merino A.,
RA Klein K., Hudak M., Dickson D., Rudi T., Gnau V., Bauch A.,
RA Bastuck S., Huhse B., Leutwein C., Heurtier M.-A., Copley R.R.,
RA Edelmann A., Querfurth E., Rybin V., Drewes G., Raida M.,
RA Bouwmeester T., Bork P., Seraphin B., Kuster B., Neubauer G.,
RA Superti-Furga G.;
RT "Functional organization of the yeast proteome by systematic analysis
RT of protein complexes.";
RL Nature 415:141-147(2002).
RN [10]
RP IDENTIFICATION IN TRAPP COMPLEX, AND INTERACTION WITH TRAPPC2L.
RX PubMed=19416478; DOI=10.1111/j.1600-0854.2009.00906.x;
RA Scrivens P.J., Shahrzad N., Moores A., Morin A., Brunet S., Sacher M.;
RT "TRAPPC2L is a novel, highly conserved TRAPP-interacting protein.";
RL Traffic 10:724-736(2009).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP IDENTIFICATION IN TRAPP COMPLEX.
RX PubMed=21525244; DOI=10.1091/mbc.E10-11-0873;
RA Scrivens P.J., Noueihed B., Shahrzad N., Hul S., Brunet S., Sacher M.;
RT "C4orf41 and TTC-15 are mammalian TRAPP components with a role at an
RT early stage in ER-to-Golgi trafficking.";
RL Mol. Biol. Cell 22:2083-2093(2011).
CC -!- FUNCTION: May play a role in vesicular transport from endoplasmic
CC reticulum to Golgi.
CC -!- SUBUNIT: Component of the multisubunit TRAPP (transport protein
CC particle) complex, which includes at least TRAPPC2, TRAPPC2L,
CC TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10,
CC TRAPPC11 and TRAPPC12. Interacts with SDC2 (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q9CQA1:Trappc5 (xeno); NbExp=2; IntAct=EBI-722888, EBI-1172213;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network
CC {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y296-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y296-2; Sequence=VSP_056348;
CC Note=No experimental confirmation available.;
CC -!- SIMILARITY: Belongs to the TRAPP small subunits family. TRAPPC4
CC subfamily. {ECO:0000305}.
DR EMBL; AF105025; AAF21897.1; -; mRNA.
DR EMBL; AF078862; AAD44494.1; -; mRNA.
DR EMBL; AF161520; AAF29135.1; -; mRNA.
DR EMBL; AF151862; AAD34099.1; -; mRNA.
DR EMBL; AK290520; BAF83209.1; -; mRNA.
DR EMBL; AK297422; BAG59853.1; -; mRNA.
DR EMBL; AP003392; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471065; EAW67430.1; -; Genomic_DNA.
DR EMBL; BC010866; AAH10866.1; -; mRNA.
DR CCDS; CCDS81633.1; -. [Q9Y296-2]
DR CCDS; CCDS8407.1; -. [Q9Y296-1]
DR RefSeq; NP_001305415.1; NM_001318486.1.
DR RefSeq; NP_001305417.1; NM_001318488.1.
DR RefSeq; NP_001305418.1; NM_001318489.1. [Q9Y296-2]
DR RefSeq; NP_001305419.1; NM_001318490.1.
DR RefSeq; NP_001305421.1; NM_001318492.1.
DR RefSeq; NP_001305423.1; NM_001318494.1.
DR RefSeq; NP_057230.1; NM_016146.5. [Q9Y296-1]
DR UniGene; Hs.132760; -.
DR PDB; 2J3T; X-ray; 2.40 A; D=1-219.
DR PDB; 2JSN; NMR; -; A=19-106.
DR PDB; 2ZMV; X-ray; 2.80 A; A/B=1-219.
DR PDBsum; 2J3T; -.
DR PDBsum; 2JSN; -.
DR PDBsum; 2ZMV; -.
DR DisProt; DP00562; -.
DR ProteinModelPortal; Q9Y296; -.
DR SMR; Q9Y296; -.
DR BioGrid; 119523; 37.
DR DIP; DIP-37871N; -.
DR IntAct; Q9Y296; 8.
DR MINT; MINT-1424006; -.
DR STRING; 9606.ENSP00000436005; -.
DR iPTMnet; Q9Y296; -.
DR PhosphoSitePlus; Q9Y296; -.
DR BioMuta; TRAPPC4; -.
DR DMDM; 20178121; -.
DR EPD; Q9Y296; -.
DR PaxDb; Q9Y296; -.
DR PeptideAtlas; Q9Y296; -.
DR PRIDE; Q9Y296; -.
DR DNASU; 51399; -.
DR Ensembl; ENST00000434101; ENSP00000405033; ENSG00000196655. [Q9Y296-2]
DR Ensembl; ENST00000533632; ENSP00000436005; ENSG00000196655. [Q9Y296-1]
DR Ensembl; ENST00000629243; ENSP00000487467; ENSG00000280495. [Q9Y296-1]
DR Ensembl; ENST00000629713; ENSP00000486496; ENSG00000280495. [Q9Y296-2]
DR GeneID; 51399; -.
DR KEGG; hsa:51399; -.
DR UCSC; uc010ryo.4; human. [Q9Y296-1]
DR CTD; 51399; -.
DR DisGeNET; 51399; -.
DR GeneCards; TRAPPC4; -.
DR HGNC; HGNC:19943; TRAPPC4.
DR HPA; HPA039752; -.
DR HPA; HPA041371; -.
DR MIM; 610971; gene.
DR neXtProt; NX_Q9Y296; -.
DR OpenTargets; ENSG00000196655; -.
DR OpenTargets; ENSG00000280495; -.
DR PharmGKB; PA134906910; -.
DR eggNOG; KOG3369; Eukaryota.
DR eggNOG; COG5122; LUCA.
DR GeneTree; ENSGT00510000047061; -.
DR HOGENOM; HOG000165618; -.
DR HOVERGEN; HBG054226; -.
DR InParanoid; Q9Y296; -.
DR KO; K20303; -.
DR OMA; DPRQSGI; -.
DR PhylomeDB; Q9Y296; -.
DR TreeFam; TF314561; -.
DR BioCyc; ZFISH:G66-32155-MONOMER; -.
DR Reactome; R-HSA-204005; COPII (Coat Protein 2) Mediated Vesicle Transport.
DR Reactome; R-HSA-3000170; Syndecan interactions.
DR Reactome; R-HSA-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR ChiTaRS; TRAPPC4; human.
DR EvolutionaryTrace; Q9Y296; -.
DR GeneWiki; TRAPPC4; -.
DR GenomeRNAi; 51399; -.
DR PRO; PR:Q9Y296; -.
DR Proteomes; UP000005640; Chromosome 11.
DR Bgee; ENSG00000196655; -.
DR CleanEx; HS_TRAPPC4; -.
DR ExpressionAtlas; Q9Y296; baseline and differential.
DR Genevisible; Q9Y296; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0030425; C:dendrite; ISS:UniProtKB.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:GOC.
DR GO; GO:0005795; C:Golgi stack; ISS:UniProtKB.
DR GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR GO; GO:0008021; C:synaptic vesicle; ISS:UniProtKB.
DR GO; GO:0030008; C:TRAPP complex; IBA:GO_Central.
DR GO; GO:0017112; F:Rab guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0048208; P:COPII vesicle coating; TAS:Reactome.
DR GO; GO:0016358; P:dendrite development; ISS:UniProtKB.
DR GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR011012; Longin-like_dom.
DR InterPro; IPR007233; TRAPPC.
DR PANTHER; PTHR23249; PTHR23249; 2.
DR Pfam; PF04099; Sybindin; 1.
DR SMART; SM01399; Sybindin; 1.
DR SUPFAM; SSF64356; SSF64356; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Complete proteome;
KW Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus;
KW Polymorphism; Reference proteome; Transport.
FT CHAIN 1 219 Trafficking protein particle complex
FT subunit 4.
FT /FTId=PRO_0000211569.
FT VAR_SEQ 81 134 Missing (in isoform 2).
FT {ECO:0000303|PubMed:14702039}.
FT /FTId=VSP_056348.
FT VARIANT 78 78 D -> A (in dbSNP:rs11640).
FT /FTId=VAR_052397.
FT STRAND 3 9 {ECO:0000244|PDB:2J3T}.
FT STRAND 15 20 {ECO:0000244|PDB:2J3T}.
FT STRAND 28 32 {ECO:0000244|PDB:2J3T}.
FT STRAND 37 42 {ECO:0000244|PDB:2J3T}.
FT STRAND 44 51 {ECO:0000244|PDB:2J3T}.
FT STRAND 54 56 {ECO:0000244|PDB:2JSN}.
FT TURN 57 60 {ECO:0000244|PDB:2JSN}.
FT STRAND 62 66 {ECO:0000244|PDB:2J3T}.
FT STRAND 79 81 {ECO:0000244|PDB:2J3T}.
FT HELIX 82 85 {ECO:0000244|PDB:2J3T}.
FT HELIX 89 91 {ECO:0000244|PDB:2J3T}.
FT STRAND 93 99 {ECO:0000244|PDB:2J3T}.
FT HELIX 105 124 {ECO:0000244|PDB:2J3T}.
FT STRAND 127 130 {ECO:0000244|PDB:2J3T}.
FT STRAND 135 141 {ECO:0000244|PDB:2J3T}.
FT STRAND 143 148 {ECO:0000244|PDB:2J3T}.
FT STRAND 154 159 {ECO:0000244|PDB:2J3T}.
FT HELIX 166 181 {ECO:0000244|PDB:2J3T}.
FT HELIX 196 209 {ECO:0000244|PDB:2J3T}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 3 207 ipfam:Sybindin [T]
FT MYHIT 3 209 ismart:Sybindin [T]
SQ SEQUENCE 219 AA; 24340 MW; C6ADE525B7CFB731 CRC64;
MAIFSVYVVN KAGGLIYQLD SYAPRAEAEK TFSYPLDLLL KLHDERVLVA FGQRDGIRVG
HAVLAINGMD VNGRYTADGK EVLEYLGNPA NYPVSIRFGR PRLTSNEKLM LASMFHSLFA
IGSQLSPEQG SSGIEMLETD TFKLHCYQTL TGIKFVVLAD PRQAGIDSLL RKIYEIYSDF
ALKNPFYSLE MPIRCELFDQ NLKLALEVAE KAGTFGPGS
//
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