Entry sw:TI100_ARATH

ID   TI100_ARATH             Reviewed;         871 AA.
AC   Q8LPR8; Q9FNJ7;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   30-NOV-2016, entry version 92.
DE   RecName: Full=Protein TIC 100 {ECO:0000303|PubMed:23372012};
DE   AltName: Full=Protein EMBRYO DEFECTIVE 1211 {ECO:0000303|PubMed:15266054};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 100 {ECO:0000303|PubMed:23372012};
DE            Short=AtTIC100 {ECO:0000303|PubMed:23372012};
GN   Name=TIC100 {ECO:0000303|PubMed:23372012};
GN   Synonyms=EMB1211 {ECO:0000303|PubMed:15266054};
GN   OrderedLocusNames=At5g22640 {ECO:0000312|TAIR:AT5G22640};
GN   ORFNames=MDJ22.6 {ECO:0000312|EMBL:BAB11670.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA   Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. II.
RT   Sequence features of the regions of 1,044,062 bp covered by thirteen
RT   physically assigned P1 clones.";
RL   DNA Res. 4:291-300(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RL   Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=15322131; DOI=10.1074/jbc.M406763200;
RA   Peltier J.-B., Ytterberg A.J., Sun Q., van Wijk K.J.;
RT   "New functions of the thylakoid membrane proteome of Arabidopsis
RT   thaliana revealed by a simple, fast, and versatile fractionation
RT   strategy.";
RL   J. Biol. Chem. 279:49367-49383(2004).
RN   [5]
RP   IDENTIFICATION.
RX   PubMed=15266054; DOI=10.1104/pp.104.045179;
RA   Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R.,
RA   Hutchens S., Sweeney T.C., McElver J., Aux G., Patton D., Meinke D.;
RT   "Identification of genes required for embryo development in
RT   Arabidopsis.";
RL   Plant Physiol. 135:1206-1220(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-649, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
RN   [7]
RP   FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=20738804; DOI=10.1111/j.1399-3054.2010.01407.x;
RA   Liang Q., Lu X., Jiang L., Wang C., Fan Y., Zhang C.;
RT   "EMB1211 is required for normal embryo development and influences
RT   chloroplast biogenesis in Arabidopsis.";
RL   Physiol. Plantarum 140:380-394(2010).
RN   [8]
RP   FUNCTION, COMPONENT OF THE 1-MD COMPLEX, SUBUNIT, IDENTIFICATION BY
RP   MASS SPECTROMETRY, DEAMIDATION AT ASN-238, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=23372012; DOI=10.1126/science.1229262;
RA   Kikuchi S., Bedard J., Hirano M., Hirabayashi Y., Oishi M., Imai M.,
RA   Takase M., Ide T., Nakai M.;
RT   "Uncovering the protein translocon at the chloroplast inner envelope
RT   membrane.";
RL   Science 339:571-574(2013).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts.
CC       May be part of an intermediate translocation complex acting as a
CC       protein-conducting channel at the inner envelope
CC       (PubMed:23372012). Plays an important role during embryogenesis
CC       and chloroplast biogenesis (PubMed:20738804).
CC       {ECO:0000269|PubMed:20738804, ECO:0000269|PubMed:23372012}.
CC   -!- SUBUNIT: Part of the Tic complex. Component of the 1-MD complex,
CC       composed of TIC20-I, TIC214, TIC100 and TIC56. Interacts with the
CC       translocating preproteins. Hydrolysis of ATP is essential for the
CC       formation of this complex (PubMed:23372012). The 1-MD complex
CC       interacts with TIC21 (By similarity).
CC       {ECO:0000250|UniProtKB:Q8GZ79, ECO:0000269|PubMed:23372012}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000269|PubMed:15322131}. Plastid, chloroplast inner membrane
CC       {ECO:0000269|PubMed:23372012}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q8LPR8-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Preferentially expressed in ovules, and
CC       moderately expressed in leaves and siliques.
CC       {ECO:0000269|PubMed:20738804}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in developing emryo.
CC       {ECO:0000269|PubMed:20738804}.
CC   -!- DISRUPTION PHENOTYPE: Severe defects during embryogenesis,
CC       producing abnormal embryos and thereby leading to a lethality of
CC       young seedlings. {ECO:0000269|PubMed:20738804,
CC       ECO:0000269|PubMed:23372012}.
CC   -!- SIMILARITY: Contains 3 MORN repeats. {ECO:0000255}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB11670.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AB006699; BAB11670.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED93056.1; -; Genomic_DNA.
DR   EMBL; AY094427; AAM19800.1; -; mRNA.
DR   RefSeq; NP_197656.2; NM_122170.3. [Q8LPR8-1]
DR   UniGene; At.8422; -.
DR   ProteinModelPortal; Q8LPR8; -.
DR   STRING; 3702.AT5G22640.1; -.
DR   TCDB; 3.A.9.1.2; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR   iPTMnet; Q8LPR8; -.
DR   PaxDb; Q8LPR8; -.
DR   ProMEX; Q8LPR8; -.
DR   EnsemblPlants; AT5G22640.1; AT5G22640.1; AT5G22640. [Q8LPR8-1]
DR   GeneID; 832327; -.
DR   Gramene; AT5G22640.1; AT5G22640.1; AT5G22640.
DR   KEGG; ath:AT5G22640; -.
DR   TAIR; AT5G22640; -.
DR   eggNOG; ENOG410IF75; Eukaryota.
DR   eggNOG; ENOG41108AB; LUCA.
DR   HOGENOM; HOG000030753; -.
DR   InParanoid; Q8LPR8; -.
DR   OMA; QGLVRYE; -.
DR   OrthoDB; EOG0936039N; -.
DR   PhylomeDB; Q8LPR8; -.
DR   PRO; PR:Q8LPR8; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q8LPR8; baseline and differential.
DR   Genevisible; Q8LPR8; AT.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR   GO; GO:0009706; C:chloroplast inner membrane; IDA:TAIR.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR   GO; GO:0009536; C:plastid; IDA:TAIR.
DR   GO; GO:0008565; F:protein transporter activity; IDA:TAIR.
DR   GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR   GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR   GO; GO:0045037; P:protein import into chloroplast stroma; IDA:TAIR.
DR   InterPro; IPR003409; MORN.
DR   Pfam; PF02493; MORN; 3.
PE   1: Evidence at protein level;
KW   Alternative splicing; Chloroplast; Coiled coil; Complete proteome;
KW   Membrane; Phosphoprotein; Plastid; Plastid inner membrane;
KW   Protein transport; Reference proteome; Repeat; Thylakoid; Transport.
FT   CHAIN         1    871       Protein TIC 100.
FT                                /FTId=PRO_0000431667.
FT   REPEAT      219    239       MORN 1. {ECO:0000255}.
FT   REPEAT      243    257       MORN 2. {ECO:0000255}.
FT   REPEAT      337    352       MORN 3. {ECO:0000255}.
FT   COILED      587    647       {ECO:0000255}.
FT   COMPBIAS    100    103       Poly-Glu. {ECO:0000255}.
FT   COMPBIAS    540    679       Glu-rich. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00007}.
FT   COMPBIAS    683    703       Asp-rich. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00004}.
FT   COMPBIAS    722    727       Poly-Ser. {ECO:0000255}.
FT   COMPBIAS    799    832       Ser-rich. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00016}.
FT   MOD_RES     238    238       Deamidated asparagine.
FT                                {ECO:0000269|PubMed:23372012}.
FT   MOD_RES     649    649       Phosphothreonine.
FT                                {ECO:0000244|PubMed:19376835}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT       243    257       ipfam:MORN [T]
FT   MYHIT       337    352       ipfam:MORN [T]
FT   MYHIT       219    239       ipfam:MORN [T]
SQ   SEQUENCE   871 AA;  99954 MW;  0FF5C2C0A90B70FC CRC64;
     MANEELTESQ QQEDPSQQLP NADEEKGSDS DSNSDSDASS QSSGDDFYIS ESENEAEGDN
     TIFNYVRPSD IPPDPNANPE TNIRRFNRVL DGKRVKRMQE EEEDKYTFYE DLFDFPRDPE
     RWKEQDLREI WADGPLEMTK PGWDPAWADE DDWDVVNDEI QEGRDPGIQP FYVPYRKPYP
     AIPDNHYDIE NAKGVVEELD RIEEFLQWVS YIFPDGSSYE GTVWDDLAQG KGVYIAENGL
     VRYEGEWLQN DMEGHGVIDV DIPDIEPIPG SKLEAKMRAE GRIIKRDYMT PEDRKWLEMD
     VEDSVALTDG NFQVPFYENE EWVTQFGEKP EKGRYRYAGQ WKHSRMHGCG VYEVNERILY
     GRFYFGELLE EEHGCTVDIC ALHSGLAEVA AAKARMFVNK PDGMIREERG PYGDPQHPYF
     YEEDDVWMAP GFINQFYEVP EYWETYVGEV DQEREMWLNS FYKAPLRLPM PAELEHWWEN
     VEVTPEFVLL NKEPEPDPND PSKLVQKEDP VILHTPTGRI INYVEDEKHG IRLFWQPPLE
     EGEEVDPSKV EFLPLGFDEF YGKEVVVKKE HPIKSFVLGI EKSVKPMLDG LEKWTEEKKK
     AYEERKEMIQ QELELVEAEI CLEEAIEDMD EELKKKEQEE EKKTEMGLTE EDEDVLVPVY
     KEEKVVTAKE KIQENKQEEK YKDDDDEDDD DGDDDDDDDD DDDLGPSSFG SADKGRRNSP
     FSSSSLSFAS CTLFPAVQSR LESSFLAWKQ HRAEPSKVNT GIIKGADTAS ASIHFPPLSS
     NNARLKMGKV ANRGCVQRSY GSSRSQSQLM SLSRLLSCNA SSSSSPPDSS SSEYLKDSGL
     WETPVGDMSV VLSLQIQTKC SDLFAETPAV S
//