ID TADA_SALTI Reviewed; 172 AA.
AC Q8XGY4; Q7AMI0;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 2.
DT 02-NOV-2016, entry version 89.
DE RecName: Full=tRNA-specific adenosine deaminase {ECO:0000255|HAMAP-Rule:MF_00972};
DE EC=3.5.4.33 {ECO:0000255|HAMAP-Rule:MF_00972};
GN Name=tadA {ECO:0000255|HAMAP-Rule:MF_00972};
GN OrderedLocusNames=STY2814, t0289;
OS Salmonella typhi.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=90370;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CT18;
RX PubMed=11677608; DOI=10.1038/35101607;
RA Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA Baker S., Basham D., Brooks K., Chillingworth T., Connerton P.,
RA Cronin A., Davis P., Davies R.M., Dowd L., White N., Farrar J.,
RA Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K.,
RA Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C.,
RA Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K.,
RA Whitehead S., Barrell B.G.;
RT "Complete genome sequence of a multiple drug resistant Salmonella
RT enterica serovar Typhi CT18.";
RL Nature 413:848-852(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700931 / Ty2;
RX PubMed=12644504; DOI=10.1128/JB.185.7.2330-2337.2003;
RA Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J.,
RA Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2
RT and CT18.";
RL J. Bacteriol. 185:2330-2337(2003).
CC -!- FUNCTION: Catalyzes the deamination of adenosine to inosine at the
CC wobble position 34 of tRNA(Arg2). {ECO:0000255|HAMAP-
CC Rule:MF_00972}.
CC -!- CATALYTIC ACTIVITY: Adenine(34) in tRNA + H(2)O = hypoxanthine(34)
CC in tRNA + NH(3). {ECO:0000255|HAMAP-Rule:MF_00972}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00972};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00972};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00972}.
CC -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC family. {ECO:0000255|HAMAP-Rule:MF_00972}.
CC -!- SIMILARITY: Contains 1 CMP/dCMP-type deaminase domain.
CC {ECO:0000255|PROSITE-ProRule:PRU01083}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO68014.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
CC Sequence=CAD02770.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
DR EMBL; AL513382; CAD02770.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE014613; AAO68014.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_457097.1; NC_003198.1.
DR ProteinModelPortal; Q8XGY4; -.
DR SMR; Q8XGY4; -.
DR STRING; 220341.STY2814; -.
DR EnsemblBacteria; AAO68014; AAO68014; t0289.
DR EnsemblBacteria; CAD02770; CAD02770; CAD02770.
DR GeneID; 1249123; -.
DR KEGG; stt:t0289; -.
DR KEGG; sty:STY2814; -.
DR PATRIC; 18543642; VBISalEnt120419_2862.
DR eggNOG; ENOG4108Z6B; Bacteria.
DR eggNOG; COG0590; LUCA.
DR HOGENOM; HOG000085050; -.
DR KO; K11991; -.
DR OMA; RIGRIFF; -.
DR Proteomes; UP000000541; Chromosome.
DR Proteomes; UP000002670; Chromosome.
DR GO; GO:0052717; F:tRNA-specific adenosine-34 deaminase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IEA:UniProtKB-HAMAP.
DR HAMAP; MF_00972; tRNA_aden_deaminase; 1.
DR InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR InterPro; IPR002125; CMP_dCMP_Zn-bd.
DR InterPro; IPR016193; Cytidine_deaminase-like.
DR InterPro; IPR028883; tRNA_aden_deaminase.
DR Pfam; PF14437; MafB19-deam; 1.
DR SUPFAM; SSF53927; SSF53927; 1.
DR PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE 3: Inferred from homology;
KW Complete proteome; Hydrolase; Metal-binding; tRNA processing; Zinc.
FT CHAIN 1 172 tRNA-specific adenosine deaminase.
FT /FTId=PRO_0000171736.
FT DOMAIN 6 117 CMP/dCMP-type deaminase.
FT {ECO:0000255|PROSITE-ProRule:PRU01083}.
FT ACT_SITE 59 59 Proton donor. {ECO:0000255|HAMAP-
FT Rule:MF_00972}.
FT METAL 57 57 Zinc; via pros nitrogen; catalytic.
FT {ECO:0000255|HAMAP-Rule:MF_00972}.
FT METAL 87 87 Zinc; catalytic. {ECO:0000255|HAMAP-
FT Rule:MF_00972}.
FT METAL 90 90 Zinc; catalytic. {ECO:0000255|HAMAP-
FT Rule:MF_00972}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 12 153 ihamap:tRNA_aden_deaminase [T]
FT MYHIT 57 94 ipat:CYT_DCMP_DEAMINASES_1 [T]
FT MYHIT 7 153 ipfam:MafB19-deam [T]
FT MYHIT 6 117 iprf:CYT_DCMP_DEAMINASES_2 [T]
SQ SEQUENCE 172 AA; 19204 MW; CD34E298DAF45F3F CRC64;
MSDVELDHEY WMRHALTLAK RAWDEREVPV GAVLVHNHRV IGEGWNRPIG RHDPTAHAEI
MALRQGGLVL QNYRLLDTTL YVTLEPCVMC AGAMVHSRIG RVVFGARDAK TGAAGSLIDV
LHHPGMNHRV EIIEGVLRDE CATLLSDFFR MRRQEIKALK KADRAEGAGP AV
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