Entry sw:RNC_CUPTR

ID   RNC_CUPTR               Reviewed;         256 AA.
AC   B3R1Z9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   18-JAN-2017, entry version 55.
DE   RecName: Full=Ribonuclease 3 {ECO:0000255|HAMAP-Rule:MF_00104};
DE            EC=3.1.26.3 {ECO:0000255|HAMAP-Rule:MF_00104};
DE   AltName: Full=Ribonuclease III {ECO:0000255|HAMAP-Rule:MF_00104};
DE            Short=RNase III {ECO:0000255|HAMAP-Rule:MF_00104};
GN   Name=rnc {ECO:0000255|HAMAP-Rule:MF_00104};
GN   OrderedLocusNames=RALTA_A2056;
OS   Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 /
OS   LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=977880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1;
RX   PubMed=18490699; DOI=10.1101/gr.076448.108;
RA   Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D.,
RA   Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M.,
RA   Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V.,
RA   Batut J., Medigue C., Masson-Boivin C.;
RT   "Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and
RT   comparative genomics of rhizobia.";
RL   Genome Res. 18:1472-1483(2008).
CC   -!- FUNCTION: Digests double-stranded RNA. Involved in the processing
CC       of primary rRNA transcript to yield the immediate precursors to
CC       the large and small rRNAs (23S and 16S). Processes some mRNAs, and
CC       tRNAs when they are encoded in the rRNA operon. Processes pre-
CC       crRNA and tracrRNA of type II CRISPR loci if present in the
CC       organism. {ECO:0000255|HAMAP-Rule:MF_00104}.
CC   -!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
CC       phosphomonoester. {ECO:0000255|HAMAP-Rule:MF_00104}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00104};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00104}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00104}.
CC   -!- SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain.
CC       {ECO:0000255|HAMAP-Rule:MF_00104}.
CC   -!- SIMILARITY: Contains 1 RNase III domain. {ECO:0000255|HAMAP-
CC       Rule:MF_00104}.
DR   EMBL; CU633749; CAQ69996.1; -; Genomic_DNA.
DR   RefSeq; WP_012353305.1; NC_010528.1.
DR   STRING; 977880.RALTA_A2056; -.
DR   EnsemblBacteria; CAQ69996; CAQ69996; RALTA_A2056.
DR   GeneID; 29762398; -.
DR   KEGG; cti:RALTA_A2056; -.
DR   PATRIC; 21530742; VBICupTai42494_2016.
DR   eggNOG; ENOG4108ZBM; Bacteria.
DR   eggNOG; COG0571; LUCA.
DR   HOGENOM; HOG000246809; -.
DR   KO; K03685; -.
DR   OMA; LTHKSCK; -.
DR   BioCyc; CTAI164546:GJNE-2051-MONOMER; -.
DR   Proteomes; UP000001692; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004525; F:ribonuclease III activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-HAMAP.
DR   GO; GO:0016075; P:rRNA catabolic process; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd00593; RIBOc; 1.
DR   Gene3D; 1.10.1520.10; -; 1.
DR   Gene3D; 3.30.160.20; -; 1.
DR   HAMAP; MF_00104; RNase_III; 1.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR011907; RNase_III.
DR   InterPro; IPR000999; RNase_III_dom.
DR   Pfam; PF00035; dsrm; 1.
DR   Pfam; PF14622; Ribonucleas_3_3; 1.
DR   SMART; SM00358; DSRM; 1.
DR   SMART; SM00535; RIBOc; 1.
DR   SUPFAM; SSF69065; SSF69065; 1.
DR   TIGRFAMs; TIGR02191; RNaseIII; 1.
DR   PROSITE; PS50137; DS_RBD; 1.
DR   PROSITE; PS00517; RNASE_3_1; 1.
DR   PROSITE; PS50142; RNASE_3_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Endonuclease; Hydrolase; Magnesium;
KW   Metal-binding; mRNA processing; Nuclease; RNA-binding;
KW   rRNA processing; rRNA-binding; tRNA processing.
FT   CHAIN         1    256       Ribonuclease 3.
FT                                /FTId=PRO_1000094105.
FT   DOMAIN        3    125       RNase III. {ECO:0000255|HAMAP-
FT                                Rule:MF_00104}.
FT   DOMAIN      152    222       DRBM. {ECO:0000255|HAMAP-Rule:MF_00104}.
FT   ACT_SITE     42     42       {ECO:0000255|HAMAP-Rule:MF_00104}.
FT   ACT_SITE    114    114       {ECO:0000255|HAMAP-Rule:MF_00104}.
FT   METAL        38     38       Magnesium. {ECO:0000255|HAMAP-
FT                                Rule:MF_00104}.
FT   METAL       111    111       Magnesium. {ECO:0000255|HAMAP-
FT                                Rule:MF_00104}.
FT   METAL       114    114       Magnesium. {ECO:0000255|HAMAP-
FT                                Rule:MF_00104}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT        18    146       ismart:RIBOc [T]
FT   MYHIT       153    221       ismart:DSRM [T]
FT   MYHIT        35     43       ipat:RNASE_3_1 [T]
FT   MYHIT         3    125       iprf:RNASE_3_2 [T]
FT   MYHIT         6    217       ihamap:RNase_III [T]
FT   MYHIT        17    138       ipfam:Ribonucleas_3_3 [T]
FT   MYHIT       153    218       ipfam:dsrm [T]
FT   MYHIT       152    222       iprf:DS_RBD [T]
SQ   SEQUENCE   256 AA;  28519 MW;  20EB73F316AA076A CRC64;
     MNLDALQQRL GYRFSKPELL QQALTHRSHS AQHNERLEFL GDSVLNCAVA DMLYGMFGKL
     DEGDLSRVRA NLVKQQALYE IAQMLQLSDT LRLGEGELKS GGFRRPSILA DALEAIVGAV
     FLDAGFDAAR ALIRKLYIPI LEQVDPRTLG KDAKTLLQEY LQGHKIALPQ YNVIATHGAA
     HSQQFEVECT VPKLEVRVFG TGASRRAAEQ AAAKLALDEV QKLVPQLLKR SRAERTGKTR
     KQPQPQDPQL SLRLKE
//