ID PUR2_ARATH Reviewed; 532 AA.
AC P52420; O04505;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 30-NOV-2016, entry version 127.
DE RecName: Full=Phosphoribosylamine--glycine ligase, chloroplastic;
DE EC=6.3.4.13;
DE AltName: Full=Glycinamide ribonucleotide synthetase;
DE Short=GARS;
DE AltName: Full=Phosphoribosylglycinamide synthetase;
DE Flags: Precursor;
GN Name=PUR2; OrderedLocusNames=At1g09830; ORFNames=F21M12.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=7920700; DOI=10.1046/j.1365-313X.1994.6010113.x;
RA Schnorr K.M., Nygaard P., Laloue M.;
RT "Molecular characterization of Arabidopsis thaliana cDNAs encoding
RT three purine biosynthetic enzymes.";
RL Plant J. 6:113-121(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RG The Arabidopsis Information Resource (TAIR);
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP
CC + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC ribose 1-diphosphate: step 2/2.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the GARS family. {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA52778.2; Type=Erroneous initiation; Evidence={ECO:0000305};
DR EMBL; X74766; CAA52778.2; ALT_INIT; mRNA.
DR EMBL; AC000132; AAB60737.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28500.1; -; Genomic_DNA.
DR EMBL; AY050820; AAK92755.1; -; mRNA.
DR EMBL; AY091422; AAM14361.1; -; mRNA.
DR PIR; E86232; E86232.
DR PIR; S37104; S37104.
DR RefSeq; NP_172454.1; NM_100857.4.
DR UniGene; At.42208; -.
DR UniGene; At.67297; -.
DR ProteinModelPortal; P52420; -.
DR SMR; P52420; -.
DR BioGrid; 22756; 1.
DR MINT; MINT-8067347; -.
DR STRING; 3702.AT1G09830.1; -.
DR PaxDb; P52420; -.
DR EnsemblPlants; AT1G09830.1; AT1G09830.1; AT1G09830.
DR GeneID; 837515; -.
DR Gramene; AT1G09830.1; AT1G09830.1; AT1G09830.
DR KEGG; ath:AT1G09830; -.
DR TAIR; AT1G09830; -.
DR eggNOG; KOG0237; Eukaryota.
DR eggNOG; COG0151; LUCA.
DR HOGENOM; HOG000033463; -.
DR InParanoid; P52420; -.
DR KO; K01945; -.
DR OMA; DEFGPPY; -.
DR OrthoDB; EOG0936071A; -.
DR PhylomeDB; P52420; -.
DR BioCyc; ARA:AT1G09830-MONOMER; -.
DR Reactome; R-ATH-73817; Purine ribonucleoside monophosphate biosynthesis.
DR UniPathway; UPA00074; UER00125.
DR PRO; PR:P52420; -.
DR Proteomes; UP000006548; Chromosome 1.
DR Genevisible; P52420; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 1.
DR Gene3D; 3.40.50.20; -; 1.
DR Gene3D; 3.90.600.10; -; 1.
DR HAMAP; MF_00138; GARS; 1.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR InterPro; IPR000115; PRibGlycinamide_synth.
DR InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR InterPro; IPR020562; PRibGlycinamide_synth_N.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR Pfam; PF01071; GARS_A; 1.
DR Pfam; PF02843; GARS_C; 1.
DR Pfam; PF02844; GARS_N; 1.
DR SMART; SM01210; GARS_C; 1.
DR SUPFAM; SSF51246; SSF51246; 1.
DR SUPFAM; SSF52440; SSF52440; 1.
DR TIGRFAMs; TIGR00877; purD; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS00184; GARS; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Complete proteome; Ligase;
KW Nucleotide-binding; Plastid; Purine biosynthesis; Reference proteome;
KW Transit peptide.
FT TRANSIT 1 ? Chloroplast. {ECO:0000255}.
FT CHAIN ? 532 Phosphoribosylamine--glycine ligase,
FT chloroplastic.
FT /FTId=PRO_0000029875.
FT DOMAIN 204 412 ATP-grasp.
FT CONFLICT 1 91 MSSLCASNCYPSSSSINLFSNNNNPTKPFLLSLRFASSNSL
FT PFVAPLKFSTTNHVLSNSRFSSNRIQRRLFLLRCVSEESQP
FT SLSIGNGGS -> LNPCLRWSESRVLYFDSLPPCRRCVHLI
FT AILHLLLSISSPTTTTQLNPFFSPSDSPPPILFHLLLLSNS
FT LLPITSLVTLVSLRTESKDAFFCSDAFQKNLSRLFLIG
FT (in Ref. 1; CAA52778). {ECO:0000305}.
FT CONFLICT 377 380 LPKL -> FLSF (in Ref. 1; CAA52778).
FT {ECO:0000305}.
FT CONFLICT 511 532 GFFRHDIGWRALRQKQVATKEE -> RFL (in Ref.
FT 1). {ECO:0000305}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 96 197 ipfam:GARS_N [T]
FT MYHIT 95 522 ihamap:GARS [T]
FT MYHIT 386 393 ipat:GARS [T]
FT MYHIT 198 392 ipfam:GARS_A [T]
FT MYHIT 426 522 ismart:GARS_C [T]
FT MYHIT 204 412 iprf:ATP_GRASP [T]
FT MYHIT 427 520 ipfam:GARS_C [T]
SQ SEQUENCE 532 AA; 56473 MW; 30C81EAB7497152B CRC64;
MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS TTNHVLSNSR
FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI GGGGREHALC HALKRSPSCD
SVLCAPGNAG ISSSGDATCV PDLDISDSLA VISFCQKWNV GLVVVGPEVP LVAGLANDLV
KAGILTFGPS SQAAALEGSK NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK
ADGLAAGKGV TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN
AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT VKGMAEEGCK
FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE SDLAKVLLAA CKGELSGVSL
DWSKDSAMVV VMASNGYPGS YEKGSIIKNL EEAERVAPGV KVFHAGTGLD SEGNVVATGG
RVLGVTAKGK DLEEARERAY SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE
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