Entry sw:PTHC_ECOLI

ID   PTHC_ECOLI              Reviewed;         187 AA.
AC   P56579; P05705; P78102; P78214;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 1.
DT   02-NOV-2016, entry version 122.
DE   RecName: Full=PTS system glucitol/sorbitol-specific EIIC component {ECO:0000303|PubMed:3553176};
DE   AltName: Full=EIIC-Gut {ECO:0000303|PubMed:3553176};
DE   AltName: Full=Glucitol/sorbitol permease IIC component {ECO:0000303|PubMed:3553176};
GN   Name=srlA; Synonyms=gutA, sbl; OrderedLocusNames=b2702, JW5429;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RX   PubMed=3553176;
RA   Yamada M., Saier M.H. Jr.;
RT   "Glucitol-specific enzymes of the phosphotransferase system in
RT   Escherichia coli. Nucleotide sequence of the gut operon.";
RL   J. Biol. Chem. 262:5455-5463(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RA   Reizer J., Reizer A., Yamada M., Saier M.H. Jr.;
RT   "The glucitol permease of Escherichia coli: a tripartite permease of
RT   the phosphotransferase system.";
RL   Microbiology 144:1463-1464(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K.,
RA   Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N.,
RA   Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H.,
RA   Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S.,
RA   Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C.,
RA   Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-
RT   K12 genome corresponding to 50.0-68.8 min on the linkage map and
RT   analysis of its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   SEQUENCE REVISION TO 21.
RX   PubMed=16397293; DOI=10.1093/nar/gkj405;
RA   Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA   Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA   Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H.,
RA   Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.;
RT   "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT   -- 2005.";
RL   Nucleic Acids Res. 34:1-9(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
RC   STRAIN=K12;
RX   PubMed=1334233; DOI=10.1038/360606a0;
RA   Wang M.X., Church G.M.;
RT   "A whole genome approach to in vivo DNA-protein interactions in E.
RT   coli.";
RL   Nature 360:606-610(1992).
RN   [8]
RP   FUNCTION, AND SUBSTRATE SPECIFICITY.
RX   PubMed=1100608;
RA   Lengeler J.;
RT   "Nature and properties of hexitol transport systems in Escherichia
RT   coli.";
RL   J. Bacteriol. 124:39-47(1975).
RN   [9]
RP   INDUCTION.
RX   PubMed=3062173; DOI=10.1016/0022-2836(88)90193-3;
RA   Yamada M., Saier M.H. Jr.;
RT   "Positive and negative regulators for glucitol (gut) operon expression
RT   in Escherichia coli.";
RL   J. Mol. Biol. 203:569-583(1988).
RN   [10]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (PTS), a major carbohydrate active
CC       transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitant with their translocation across the cell
CC       membrane. The enzyme II complex composed of SrlA, SrlB and SrlE is
CC       involved in glucitol/sorbitol transport. It can also use D-
CC       mannitol. {ECO:0000269|PubMed:1100608}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00430, ECO:0000305|PubMed:3553176}; Multi-pass membrane
CC       protein {ECO:0000255|PROSITE-ProRule:PRU00430,
CC       ECO:0000305|PubMed:3553176}.
CC   -!- INDUCTION: Regulated by an unusual system which consists of the
CC       activator GutM and the repressor GutR in addition to the cAMP-CRP
CC       complex. {ECO:0000269|PubMed:3062173}.
CC   -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC       and contains the specific substrate-binding site.
CC       {ECO:0000255|PROSITE-ProRule:PRU00430}.
CC   -!- SIMILARITY: Contains 1 PTS EIIC type-5 domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00430}.
DR   EMBL; J02708; AAC13411.1; -; Genomic_DNA.
DR   EMBL; U00096; AAT48148.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA18886.2; -; Genomic_DNA.
DR   EMBL; X70016; CAA49614.1; -; Genomic_DNA.
DR   PIR; A26725; WQEC2S.
DR   RefSeq; WP_000573321.1; NZ_LN832404.1.
DR   RefSeq; YP_026180.1; NC_000913.3.
DR   ProteinModelPortal; P56579; -.
DR   SMR; P56579; -.
DR   BioGrid; 4262078; 18.
DR   IntAct; P56579; 1.
DR   STRING; 511145.b2702; -.
DR   TCDB; 4.A.4.1.1; the pts glucitol (gut) family.
DR   PaxDb; P56579; -.
DR   PRIDE; P56579; -.
DR   EnsemblBacteria; AAT48148; AAT48148; b2702.
DR   EnsemblBacteria; BAA18886; BAA18886; BAA18886.
DR   GeneID; 947575; -.
DR   KEGG; ecj:JW5429; -.
DR   KEGG; eco:b2702; -.
DR   PATRIC; 32120802; VBIEscCol129921_2793.
DR   EchoBASE; EB0962; -.
DR   EcoGene; EG10969; srlA.
DR   eggNOG; ENOG410787A; Bacteria.
DR   eggNOG; COG3730; LUCA.
DR   HOGENOM; HOG000242767; -.
DR   InParanoid; P56579; -.
DR   KO; K02783; -.
DR   OMA; HINPGEY; -.
DR   PhylomeDB; P56579; -.
DR   BioCyc; EcoCyc:G8210-MONOMER; -.
DR   BioCyc; ECOL316407:JW5429-MONOMER; -.
DR   BioCyc; MetaCyc:G8210-MONOMER; -.
DR   PRO; PR:P56579; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IDA:EcoCyc.
DR   InterPro; IPR004699; PTS_IID_sorb.
DR   Pfam; PF03608; EII-GUT; 1.
DR   PIRSF; PIRSF038321; PTS_glc_srb_IIC; 1.
DR   TIGRFAMs; TIGR00821; EII-GUT; 1.
DR   PROSITE; PS51107; PTS_EIIC_TYPE_5; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Complete proteome; Membrane;
KW   Phosphotransferase system; Reference proteome; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    187       PTS system glucitol/sorbitol-specific
FT                                EIIC component.
FT                                /FTId=PRO_0000186565.
FT   TOPO_DOM      1     28       Periplasmic. {ECO:0000255}.
FT   TRANSMEM     29     49       Helical. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00430}.
FT   TOPO_DOM     50     67       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM     68     88       Helical. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00430}.
FT   TOPO_DOM     89    131       Periplasmic. {ECO:0000255}.
FT   TRANSMEM    132    152       Helical. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00430}.
FT   TOPO_DOM    153    187       Cytoplasmic. {ECO:0000255}.
FT   DOMAIN        1    187       PTS EIIC type-5. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00430}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT         5    171       ipfam:EII-GUT [T]
FT   MYHIT         1    187       iprf:PTS_EIIC_TYPE_5 [T]
SQ   SEQUENCE   187 AA;  20580 MW;  86A10715BD9DE287 CRC64;
     MIETITHGAE WFIGLFQKGG EVFTGMVTGI LPLLISLLVI MNALINFIGQ HRIERFAQRC
     AGNPVSRYLL LPCIGTFVFC NPMTLSLGRF MPEKYKPSYY AAASYSCHSM NGLFPHINPG
     ELFVYLGIAS GLTTLNLPLG PLAVSYLLVG LVTNFFRGWV TDLTTAIFEK KMGIQLEQKV
     HLAGATS
//