ID PEF1_MOUSE Reviewed; 275 AA.
AC Q8BFY6; Q8VCT5; Q9CYW8; Q9D934;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 30-NOV-2016, entry version 116.
DE RecName: Full=Peflin;
DE AltName: Full=PEF protein with a long N-terminal hydrophobic domain;
DE AltName: Full=Penta-EF hand domain-containing protein 1;
GN Name=Pef1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and
RT expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Heterodimer; heterodimerizes with PDCD6. Dissociates from
CC PDCD6 in presence of Ca(2+) (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane
CC protein. Note=Membrane-associated in the presence of Ca(2+).
CC {ECO:0000250}.
CC -!- SIMILARITY: Contains 5 EF-hand domains. {ECO:0000255|PROSITE-
CC ProRule:PRU00448}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB28735.1; Type=Frameshift; Positions=248; Evidence={ECO:0000305};
DR EMBL; AK007394; BAB25010.1; -; mRNA.
DR EMBL; AK013238; BAB28735.1; ALT_FRAME; mRNA.
DR EMBL; AK046247; BAC32654.1; -; mRNA.
DR EMBL; AK075978; BAC36091.1; -; mRNA.
DR EMBL; BC019191; AAH19191.1; -; mRNA.
DR CCDS; CCDS18706.1; -.
DR RefSeq; NP_080717.2; NM_026441.4.
DR UniGene; Mm.20818; -.
DR ProteinModelPortal; Q8BFY6; -.
DR SMR; Q8BFY6; -.
DR STRING; 10090.ENSMUSP00000030563; -.
DR PhosphoSitePlus; Q8BFY6; -.
DR EPD; Q8BFY6; -.
DR MaxQB; Q8BFY6; -.
DR PaxDb; Q8BFY6; -.
DR PeptideAtlas; Q8BFY6; -.
DR PRIDE; Q8BFY6; -.
DR Ensembl; ENSMUST00000030563; ENSMUSP00000030563; ENSMUSG00000028779.
DR Ensembl; ENSMUST00000118199; ENSMUSP00000112638; ENSMUSG00000028779.
DR GeneID; 67898; -.
DR KEGG; mmu:67898; -.
DR UCSC; uc008uyw.1; mouse.
DR CTD; 553115; -.
DR MGI; MGI:1915148; Pef1.
DR eggNOG; KOG0037; Eukaryota.
DR eggNOG; ENOG410YKQK; LUCA.
DR GeneTree; ENSGT00620000087734; -.
DR HOGENOM; HOG000231983; -.
DR HOVERGEN; HBG004492; -.
DR InParanoid; Q8BFY6; -.
DR OMA; FTNSNAM; -.
DR OrthoDB; EOG091G0ISY; -.
DR PhylomeDB; Q8BFY6; -.
DR TreeFam; TF314682; -.
DR PRO; PR:Q8BFY6; -.
DR Proteomes; UP000000589; Chromosome 4.
DR Bgee; ENSMUSG00000028779; -.
DR CleanEx; MM_PEF1; -.
DR Genevisible; Q8BFY6; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0070062; C:extracellular exosome; ISO:MGI.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0044822; F:poly(A) RNA binding; ISO:MGI.
DR GO; GO:0046983; F:protein dimerization activity; ISO:MGI.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:MGI.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR GO; GO:0051592; P:response to calcium ion; ISO:MGI.
DR CDD; cd00051; EFh; 2.
DR Gene3D; 1.10.238.10; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13405; EF-hand_6; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Calcium; Complete proteome; Cytoplasm; Membrane; Metal-binding;
KW Reference proteome; Repeat.
FT CHAIN 1 275 Peflin.
FT /FTId=PRO_0000247046.
FT REPEAT 21 29 1.
FT REPEAT 31 39 2.
FT REPEAT 41 49 3.
FT REPEAT 50 59 4.
FT REPEAT 60 68 5.
FT REPEAT 76 84 6.
FT REPEAT 85 91 7.
FT REPEAT 92 100 8.
FT DOMAIN 105 140 EF-hand 1. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 146 174 EF-hand 2. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 172 207 EF-hand 3. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 208 244 EF-hand 4. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 245 274 EF-hand 5. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT CA_BIND 118 129 1. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT CA_BIND 185 196 2. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT REGION 21 100 8 X 9 AA approximate tandem repeat of
FT [AP]-P-G-G-P-Y-G-G-P-P.
FT REGION 195 275 Required for interaction with PDCD6.
FT {ECO:0000250}.
FT CONFLICT 148 148 L -> H (in Ref. 2; AAH19191).
FT {ECO:0000305}.
FT CONFLICT 218 218 S -> F (in Ref. 1; BAB28735).
FT {ECO:0000305}.
FT CONFLICT 257 257 G -> R (in Ref. 1; BAB25010).
FT {ECO:0000305}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 177 203 ipfam:EF-hand_6 [T]
FT MYHIT 109 137 ismart:EFh [T]
FT MYHIT 172 207 iprf:EF_HAND_2 [T]
FT MYHIT 185 197 ipat:EF_HAND_1 [T]
FT MYHIT 146 174 ismart:EFh [T]
FT MYHIT 176 204 ismart:EFh [T]
FT MYHIT 105 140 iprf:EF_HAND_2 [T]
FT MYHIT 113 134 ipfam:EF-hand_5 [T]
FT MYHIT 118 130 ipat:EF_HAND_1 [T]
SQ SEQUENCE 275 AA; 29228 MW; DE4782B47ABEE9E2 CRC64;
MASYPNGQSC PGAAGQVPGV PPGGYYPGPP HGGGQYGSGL PPGGGYGAPA PGGPYGYPSA
GGVPSGTPSG PYGGIPPGGP YGQLPPGGPY GTQPGHYGQG GVPPNVDPEA YSWFQSVDAD
HSGYISLKEL KQALVNSNWS SFNDETCLMM INMFDKTKSG RIDVAGFSAL WKFLQQWRNL
FQQYDRDRSG SISSTELQQA LSQMGYNLSP QFTQLLVSRY CARSAIPAMQ LDCFIKVCTQ
LQVLTEAFRE KDTAVQGNIR LSFEDFVTMT ASRML
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