ID PEF1_HUMAN Reviewed; 284 AA.
AC Q9UBV8;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 30-NOV-2016, entry version 146.
DE RecName: Full=Peflin;
DE AltName: Full=PEF protein with a long N-terminal hydrophobic domain;
DE AltName: Full=Penta-EF hand domain-containing protein 1;
GN Name=PEF1; Synonyms=ABP32; ORFNames=UNQ1845/PRO3573;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10486255; DOI=10.1006/bbrc.1999.1189;
RA Kitaura Y., Watanabe M., Satoh H., Kawai T., Hitomi K., Maki M.;
RT "Peflin, a novel member of the five-EF-hand-protein family, is similar
RT to the apoptosis-linked gene 2 (ALG-2) protein but possesses
RT nonapeptide repeats in the N-terminal hydrophobic region.";
RL Biochem. Biophys. Res. Commun. 263:68-75(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Spleen;
RA Shibata M., Noguchi J.;
RT "A putative calcium binding protein that has five EF-hand motifs.";
RL Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH PDCD6.
RX PubMed=11278427; DOI=10.1074/jbc.M008649200;
RA Kitaura Y., Matsumoto S., Satoh H., Hitomi K., Maki M.;
RT "Peflin and ALG-2, members of the penta-EF-hand protein family, form a
RT heterodimer that dissociates in a Ca2+-dependent manner.";
RL J. Biol. Chem. 276:14053-14058(2001).
RN [8]
RP INTERACTION WITH PDCD6.
RX PubMed=11883899; DOI=10.1006/abbi.2001.2736;
RA Kitaura Y., Satoh H., Takahashi H., Shibata H., Maki M.;
RT "Both ALG-2 and peflin, penta-EF-hand (PEF) proteins, are stabilized
RT by dimerization through their fifth EF-hand regions.";
RL Arch. Biochem. Biophys. 399:12-18(2002).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- SUBUNIT: Heterodimer; heterodimerizes with PDCD6. Dissociates from
CC PDCD6 in presence of Ca(2+).
CC -!- INTERACTION:
CC Q13137:CALCOCO2; NbExp=4; IntAct=EBI-724639, EBI-739580;
CC D3DR37:CEP55; NbExp=3; IntAct=EBI-724639, EBI-10173536;
CC Q15038:DAZAP2; NbExp=3; IntAct=EBI-724639, EBI-724310;
CC Q01844:EWSR1; NbExp=3; IntAct=EBI-724639, EBI-739737;
CC Q6PJQ5:FOXR2; NbExp=3; IntAct=EBI-724639, EBI-8468543;
CC O14964:HGS; NbExp=3; IntAct=EBI-724639, EBI-740220;
CC Q53G59:KLHL12; NbExp=7; IntAct=EBI-724639, EBI-740929;
CC O75340:PDCD6; NbExp=11; IntAct=EBI-724639, EBI-352915;
CC Q92734:TFG; NbExp=3; IntAct=EBI-724639, EBI-357061;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11278427}.
CC Membrane {ECO:0000269|PubMed:11278427}; Peripheral membrane
CC protein {ECO:0000269|PubMed:11278427}. Note=Membrane-associated in
CC the presence of Ca(2+).
CC -!- SIMILARITY: Contains 5 EF-hand domains. {ECO:0000255|PROSITE-
CC ProRule:PRU00448}.
DR EMBL; AB026628; BAA85163.1; -; mRNA.
DR EMBL; AB018357; BAA84922.1; -; mRNA.
DR EMBL; AY359011; AAQ89370.1; -; mRNA.
DR EMBL; AK001420; BAA91680.1; -; mRNA.
DR EMBL; CR542139; CAG46936.1; -; mRNA.
DR EMBL; BC002773; AAH02773.1; -; mRNA.
DR EMBL; BC012561; AAH12561.1; -; mRNA.
DR CCDS; CCDS345.1; -.
DR RefSeq; NP_036524.1; NM_012392.3.
DR UniGene; Hs.470417; -.
DR ProteinModelPortal; Q9UBV8; -.
DR SMR; Q9UBV8; -.
DR BioGrid; 139275; 28.
DR IntAct; Q9UBV8; 23.
DR MINT; MINT-5003645; -.
DR STRING; 9606.ENSP00000362807; -.
DR iPTMnet; Q9UBV8; -.
DR PhosphoSitePlus; Q9UBV8; -.
DR BioMuta; PEF1; -.
DR DMDM; 74761895; -.
DR REPRODUCTION-2DPAGE; IPI00018235; -.
DR EPD; Q9UBV8; -.
DR MaxQB; Q9UBV8; -.
DR PaxDb; Q9UBV8; -.
DR PeptideAtlas; Q9UBV8; -.
DR PRIDE; Q9UBV8; -.
DR DNASU; 553115; -.
DR Ensembl; ENST00000373703; ENSP00000362807; ENSG00000162517.
DR GeneID; 553115; -.
DR KEGG; hsa:553115; -.
DR UCSC; uc001bth.3; human.
DR CTD; 553115; -.
DR GeneCards; PEF1; -.
DR HGNC; HGNC:30009; PEF1.
DR HPA; HPA061608; -.
DR MIM; 610033; gene.
DR neXtProt; NX_Q9UBV8; -.
DR OpenTargets; ENSG00000162517; -.
DR PharmGKB; PA142671184; -.
DR eggNOG; KOG0037; Eukaryota.
DR eggNOG; ENOG410YKQK; LUCA.
DR GeneTree; ENSGT00620000087734; -.
DR HOGENOM; HOG000231983; -.
DR HOVERGEN; HBG004492; -.
DR InParanoid; Q9UBV8; -.
DR OMA; PSMQLDR; -.
DR OrthoDB; EOG091G0ISY; -.
DR PhylomeDB; Q9UBV8; -.
DR TreeFam; TF314682; -.
DR BioCyc; ZFISH:ENSG00000162517-MONOMER; -.
DR GeneWiki; PEF1; -.
DR GenomeRNAi; 553115; -.
DR PRO; PR:Q9UBV8; -.
DR Proteomes; UP000005640; Chromosome 1.
DR Bgee; ENSG00000162517; -.
DR CleanEx; HS_PEF1; -.
DR Genevisible; Q9UBV8; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; TAS:ProtInc.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0044822; F:poly(A) RNA binding; IDA:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IPI:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:UniProtKB.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR GO; GO:0051592; P:response to calcium ion; IPI:UniProtKB.
DR CDD; cd00051; EFh; 2.
DR Gene3D; 1.10.238.10; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13405; EF-hand_6; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Calcium; Complete proteome; Cytoplasm; Membrane; Metal-binding;
KW Reference proteome; Repeat.
FT CHAIN 1 284 Peflin.
FT /FTId=PRO_0000247045.
FT REPEAT 21 29 1.
FT REPEAT 31 39 2.
FT REPEAT 41 49 3.
FT REPEAT 50 58 4.
FT REPEAT 59 67 5.
FT REPEAT 76 84 6.
FT REPEAT 85 92 7.
FT REPEAT 93 100 8.
FT REPEAT 101 109 9.
FT DOMAIN 114 149 EF-hand 1. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 155 183 EF-hand 2. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 181 216 EF-hand 3. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 217 253 EF-hand 4. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT DOMAIN 254 283 EF-hand 5. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT CA_BIND 127 138 1. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT CA_BIND 194 205 2. {ECO:0000255|PROSITE-
FT ProRule:PRU00448}.
FT REGION 21 109 9 X 9 AA approximate tandem repeat of
FT [AP]-P-G-G-P-Y-G-G-P-P.
FT REGION 204 284 Required for interaction with PDCD6.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 194 206 ipat:EF_HAND_1 [T]
FT MYHIT 155 183 ismart:EFh [T]
FT MYHIT 127 139 ipat:EF_HAND_1 [T]
FT MYHIT 186 212 ipfam:EF-hand_6 [T]
FT MYHIT 114 149 iprf:EF_HAND_2 [T]
FT MYHIT 185 213 ismart:EFh [T]
FT MYHIT 120 179 ipfam:EF-hand_7 [T]
FT MYHIT 181 216 iprf:EF_HAND_2 [T]
FT MYHIT 118 146 ismart:EFh [T]
SQ SEQUENCE 284 AA; 30381 MW; 2E9AA5750CB7A68A CRC64;
MASYPYRQGC PGAAGQAPGA PPGSYYPGPP NSGGQYGSGL PPGGGYGGPA PGGPYGPPAG
GGPYGHPNPG MFPSGTPGGP YGGAAPGGPY GQPPPSSYGA QQPGLYGQGG APPNVDPEAY
SWFQSVDSDH SGYISMKELK QALVNCNWSS FNDETCLMMI NMFDKTKSGR IDVYGFSALW
KFIQQWKNLF QQYDRDRSGS ISYTELQQAL SQMGYNLSPQ FTQLLVSRYC PRSANPAMQL
DRFIQVCTQL QVLTEAFREK DTAVQGNIRL SFEDFVTMTA SRML
//
|
