Legends: 1, Iron-sulfur 1 (4Fe-4S). {ECO:0000250|UniProtKB:P94692}; 2, Iron-sulfur 2 (4Fe-4S). {ECO:0000250|UniProtKB:P94692}; 3, Iron-sulfur 3 (4Fe-4S). {ECO:0000250|UniProtKB:P94692}; 4, 4Fe-4S ferredoxin-type 1. {ECO:0000255|PROSITE-ProRule:PRU00711}; 5, 4Fe-4S ferredoxin-type 2. {ECO:0000255|PROSITE-ProRule:PRU00711}; 6, ipfam:TPP_enzyme_C [T]; 7, iprf:4FE4S_FER_2 [T]; 8, ismart:EKR [T]; 9, ipfam:PFOR_II [T]; 10, ipat:4FE4S_FER_1 [T]; 11, ipfam:EKR [T].
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ID NIFJ_ENTAG Reviewed; 1173 AA.
AC P19543;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 3.
DT 02-NOV-2016, entry version 95.
DE RecName: Full=Pyruvate-flavodoxin oxidoreductase;
DE EC=1.2.7.-;
GN Name=nifJ;
OS Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans).
OG Plasmid pEA3.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX NCBI_TaxID=549;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=333;
RA Schwickerath O.;
RL Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128.
RC STRAIN=333;
RX PubMed=2504647; DOI=10.1016/0378-1119(89)90318-1;
RA Kreutzer R., Singh M., Klingmueller W.;
RT "Identification and characterization of the nifH and nifJ promoter
RT regions located on the nif-plasmid pEA3 of Enterobacter agglomerans
RT 333.";
RL Gene 78:101-109(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1028-1173.
RC STRAIN=333;
RX PubMed=1708766;
RA Kreutzer R., Dayananda S., Klingmueller W.;
RT "Cotranscription of the electron transport protein genes nifJ and nifF
RT in Enterobacter agglomerans 333.";
RL J. Bacteriol. 173:3252-3256(1991).
CC -!- FUNCTION: Oxidoreductase required for the transfer of electrons
CC from pyruvate to flavodoxin, which reduces nitrogenase.
CC -!- CATALYTIC ACTIVITY: Pyruvate + CoA + oxidized flavodoxin = acetyl-
CC CoA + CO(2) + reduced flavodoxin.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000250|UniProtKB:P94692};
CC Note=Binds 3 [4Fe-4S] clusters per subunit.
CC {ECO:0000250|UniProtKB:P94692};
CC -!- SIMILARITY: Belongs to the NifJ family. {ECO:0000305}.
CC -!- SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.
CC {ECO:0000255|PROSITE-ProRule:PRU00711}.
DR EMBL; X78558; CAA55302.1; -; Genomic_DNA.
DR EMBL; X99694; CAA68011.1; -; Genomic_DNA.
DR EMBL; M26931; AAA24806.2; -; Genomic_DNA.
DR EMBL; M38221; AAA23386.1; -; Genomic_DNA.
DR PIR; B39414; B39414.
DR ProteinModelPortal; P19543; -.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016903; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors; IEA:InterPro.
DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR GO; GO:0022900; P:electron transport chain; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.920; -; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR Gene3D; 3.40.920.10; -; 1.
DR Gene3D; 4.10.780.10; -; 1.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR033412; PFOR_II.
DR InterPro; IPR019456; Pyrv-flavodox_OxRtase_EKR.
DR InterPro; IPR019752; Pyrv/ketoisovalerate_OxRed_cat.
DR InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
DR InterPro; IPR011895; Pyrv_flavodox_OxRed.
DR InterPro; IPR002869; Pyrv_flavodox_OxRed_cen.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR011766; TPP_enzyme-bd_C.
DR InterPro; IPR009014; Transketo_C/PFOR_II.
DR Pfam; PF10371; EKR; 1.
DR Pfam; PF17147; PFOR_II; 1.
DR Pfam; PF01558; POR; 1.
DR Pfam; PF01855; POR_N; 1.
DR Pfam; PF02775; TPP_enzyme_C; 1.
DR PIRSF; PIRSF000159; NifJ; 1.
DR SMART; SM00890; EKR; 1.
DR SUPFAM; SSF52518; SSF52518; 2.
DR SUPFAM; SSF52922; SSF52922; 1.
DR SUPFAM; SSF53323; SSF53323; 1.
DR TIGRFAMs; TIGR02176; pyruv_ox_red; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 2.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 3: Inferred from homology;
KW 4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding;
KW Nitrogen fixation; Oxidoreductase; Plasmid; Repeat; Transport.
FT CHAIN 1 1173 Pyruvate-flavodoxin oxidoreductase.
FT /FTId=PRO_0000215554.
FT DOMAIN 681 710 4Fe-4S ferredoxin-type 1.
FT {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT DOMAIN 735 766 4Fe-4S ferredoxin-type 2.
FT {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT METAL 690 690 Iron-sulfur 1 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 693 693 Iron-sulfur 1 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 696 696 Iron-sulfur 1 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 700 700 Iron-sulfur 2 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 744 744 Iron-sulfur 2 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 747 747 Iron-sulfur 2 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 750 750 Iron-sulfur 2 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 754 754 Iron-sulfur 1 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 810 810 Iron-sulfur 3 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 813 813 Iron-sulfur 3 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 838 838 Iron-sulfur 3 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
FT METAL 1075 1075 Iron-sulfur 3 (4Fe-4S).
FT {ECO:0000250|UniProtKB:P94692}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 964 1070 ipfam:TPP_enzyme_C [T]
FT MYHIT 681 710 iprf:4FE4S_FER_2 [T]
FT MYHIT 622 680 ismart:EKR [T]
FT MYHIT 272 374 ipfam:PFOR_II [T]
FT MYHIT 744 755 ipat:4FE4S_FER_1 [T]
FT MYHIT 735 766 iprf:4FE4S_FER_2 [T]
FT MYHIT 625 679 ipfam:EKR [T]
FT MYHIT 18 247 ipfam:POR_N [T]
FT MYHIT 426 610 ipfam:POR [T]
SQ SEQUENCE 1173 AA; 128246 MW; 0B35809565C5DE4B CRC64;
MPGKMKTMDG NTAAAYVSYA FTDVTAIYPI TPSTPMAESV DEWAAQGKKN LFGQTVKIME
MQSESGAAGA IHGALQAGAL ATTYTASQGL LLMTPNMYKI AGELLPAVFH VSARALATNS
LNIFGDHQDS YAVRHTGCAM LAESSVQQVM DLAAVAHLTA IKGRIPFINF FDGFRTSHEI
QKIEVLDYED LAHLLDRDAV ARFRQNALHP DHPVVRGTAQ NPDIYFQERE ASNKFYMALP
EMVESYMAKI SALTGREYHL FNYYGAPDAG RMIIAMGSVC ETIQETVDYL NARGEKVGVL
TVHLYRPFSL KHFFKYIPKS VSNIAVLDRT KEPGSLAEPL YLDVKSAFYN SDWRPVIVGG
RYALGGKDIL PSHIISIFDN LAAERPRDGF TVGINDDVTF TSLPLSDRDI DTSSSGTTAC
KFWGLGSDGT VGANKSAIKI IGDKTDMYAQ AYFAYDSKKS GGVTMSHLRF GHNPIRSPYL
IDKADFISCS QQSYVNKYHL LAGLKPGGTF LLNCSWDVAE LDEKLPVAMK RYIAANDIQF
YIVNAVGIAQ KLGLGGRFNM IMQSAFFKLA DIIPLTRAVE YLKQSIEHAY GNKGQKIVDM
NNLAVDSGIE SVVKISVPQA WKHLEDKVVS PKKLPAFIKD ILIPMNRQEG DSLPVSIFDG
IEDGTFPSGT SAYEKRGVAI NVPVWQTDKC TQCNQCAFIC PHAAIRPVLI SEEERQNAPA
GFSAKRASGT EDAWYRLAVS PLDCSGCGNC ADVCPVKGKA LSMQPLESQE HEIELWEYAL
SLTPKANPQN KFTVKGSQFE QPLLEFSGAC GGCGETPYAK LVTQLFGDRM MIANATGCSS
IWGASAPSIP YTTNHKGQGP TWANSLFEDN AEFGLGMLLG VDAIRDTLAT QVKAALDNAP
DVPLDAELSA CLSDWLANKD QGEGTRERAE KVGDTSGFAN AREKSRTPTV SMPHRDYLAN
GSHWIFGGDG WAYDIGFGGL DHVLASGKDV NVLVFDTEVY SNTGGQSSKS TPAAAIAQFA
CKKTRKKDLG MMAMSYGYVY VAQIAMGADK NQTLRAIAEA EAYPGPSLII AYAACINHGL
RIGMGCSQRE ARRAVEAGYW ANYRYHPELK EAGKNPFILD SEEPEEDFQA FLAGEVRYSS
LKKLYPEFAE FLFKKTEDDA RERLEGYKKL ASS
//
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