ID MOA1_SCHPO Reviewed; 172 AA.
AC Q9UTI4;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 02-NOV-2016, entry version 83.
DE RecName: Full=Monopolar attachment protein 1;
DE AltName: Full=Meiotically up-regulated gene 159 protein;
GN Name=moa1; Synonyms=mug159; ORFNames=SPAC15E1.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales;
OC Schizosaccharomycetaceae; Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND INTERACTION WITH REC8.
RX PubMed=16325576; DOI=10.1016/j.cell.2005.09.013;
RA Yokobayashi S., Watanabe Y.;
RT "The kinetochore protein moa1 enables cohesion-mediated monopolar
RT attachment at meiosis I.";
RL Cell 123:803-817(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L.,
RA San-Segundo P., Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes
RT required for critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the
RT fission yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [5]
RP FUNCTION, INTERACTION WITH PLO1, SUBCELLULAR LOCATION, AND MUTAGENESIS
RP OF THR-101.
RX PubMed=25533956; DOI=10.1038/nature14097;
RA Kim J., Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A.,
RA Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S.,
RA Tanno Y., Sakuno T., Watanabe Y.;
RT "Meikin is a conserved regulator of meiosis-I-specific kinetochore
RT function.";
RL Nature 517:466-471(2015).
CC -!- FUNCTION: Plays an important role in chromosome segregation during
CC meiosis I by allowing meiotic rec8 to establish cohesion at the
CC centromeric central core and thereby promote the side-by-side
CC structure of kinetochores at meiosis I. Enables monopolar
CC attachment during meiosis I (PubMed:16303567, PubMed:16325576).
CC Required to facilitate kinetochore mono-orientation during meiosis
CC I, when kinetochores on sister chromosomes face the same direction
CC and are thus captured and pulled by spindle fibers from the same
CC pole (PubMed:25533956). Acts in collaboration with plo1
CC (PubMed:25533956). {ECO:0000269|PubMed:16303567,
CC ECO:0000269|PubMed:16325576, ECO:0000269|PubMed:25533956}.
CC -!- SUBUNIT: Interacts with rec8 (PubMed:16325576), Interacts with
CC plo1 (PubMed:25533956). {ECO:0000269|PubMed:16325576,
CC ECO:0000269|PubMed:25533956}.
CC -!- INTERACTION:
CC P36626:rec8; NbExp=2; IntAct=EBI-1564258, EBI-1564281;
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere
CC {ECO:0000269|PubMed:16325576}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:16325576, ECO:0000305|PubMed:25533956}.
CC Note=Localizes to the centromere. Does not require rec8 for
CC centromeric localization but instead cnp3.
CC {ECO:0000269|PubMed:16325576}.
CC -!- DEVELOPMENTAL STAGE: Present during meiotic prophase I, when
CC monopolar attachment of kinetochores to spindle microtubules is
CC established. First appears during prophase I and localizes to
CC several punctate dots in the nucleus until metaphase I. At the
CC onset of anaphase I, it decreases markedly and remains absent
CC until telophase I. Does not reappear during meiosis II.
CC {ECO:0000269|PubMed:16325576}.
DR EMBL; AB232928; BAE46564.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB52426.1; -; Genomic_DNA.
DR PIR; T37722; T37722.
DR RefSeq; NP_594308.1; NM_001019731.1.
DR ProteinModelPortal; Q9UTI4; -.
DR SMR; Q9UTI4; -.
DR BioGrid; 279256; 5.
DR IntAct; Q9UTI4; 2.
DR PRIDE; Q9UTI4; -.
DR EnsemblFungi; SPAC15E1.07c.1; SPAC15E1.07c.1:pep; SPAC15E1.07c.
DR GeneID; 2542808; -.
DR KEGG; spo:SPAC15E1.07c; -.
DR EuPathDB; FungiDB:SPAC15E1.07c; -.
DR PomBase; SPAC15E1.07c; moa1.
DR OrthoDB; EOG092C5H2T; -.
DR PRO; PR:Q9UTI4; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000941; C:condensed nuclear chromosome inner kinetochore; IDA:PomBase.
DR GO; GO:0034991; C:nuclear meiotic cohesin complex; NAS:PomBase.
DR GO; GO:0005634; C:nucleus; NAS:PomBase.
DR GO; GO:0051455; P:attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation; IMP:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034613; P:cellular protein localization; IMP:PomBase.
DR GO; GO:0061171; P:establishment of bipolar cell polarity; IGI:PomBase.
DR GO; GO:1990735; P:gamma-tubulin complex localization to mitotic spindle pole body; IMP:PomBase.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:PomBase.
DR GO; GO:1990813; P:meiotic centromeric cohesion protection; IGI:PomBase.
DR GO; GO:0045132; P:meiotic chromosome segregation; IMP:PomBase.
DR GO; GO:0051177; P:meiotic sister chromatid cohesion; IMP:PomBase.
DR GO; GO:0090307; P:mitotic spindle assembly; IMP:PomBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome;
KW Chromosome partition; Complete proteome; Kinetochore; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1 172 Monopolar attachment protein 1.
FT /FTId=PRO_0000076264.
FT MOTIF 100 102 POLO box domain (PBD)-binding.
FT {ECO:0000305|PubMed:25533956}.
FT MUTAGEN 101 101 T->A: Abolition of meiosis-specific
FT enrichment of plo1 to kinetochores.
FT {ECO:0000269|PubMed:25533956}.
SQ SEQUENCE 172 AA; 19612 MW; BAC48C2E18157031 CRC64;
MAINNENELE YKLIKKNKNP KISNSKKKNS TRPALQDKTN QTLPIHQNQA FSNILPSDFS
IIKTPETKTA DDFPVNGYEG LNILKFDLEL FYKLKPVATS TPKSCMRTGS NLFLNETVKH
VPDERLVSNI KNTQTKDSIT RDSAYYHRKT MTESIIKTLA AFDAEVDEII LF
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