ID MIAA_THERP Reviewed; 329 AA.
AC B9L505;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 02-NOV-2016, entry version 51.
DE RecName: Full=tRNA dimethylallyltransferase {ECO:0000255|HAMAP-Rule:MF_00185};
DE EC=2.5.1.75 {ECO:0000255|HAMAP-Rule:MF_00185};
DE AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase {ECO:0000255|HAMAP-Rule:MF_00185};
DE Short=DMAPP:tRNA dimethylallyltransferase {ECO:0000255|HAMAP-Rule:MF_00185};
DE Short=DMATase {ECO:0000255|HAMAP-Rule:MF_00185};
DE AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase {ECO:0000255|HAMAP-Rule:MF_00185};
DE Short=IPP transferase {ECO:0000255|HAMAP-Rule:MF_00185};
DE Short=IPPT {ECO:0000255|HAMAP-Rule:MF_00185};
DE Short=IPTase {ECO:0000255|HAMAP-Rule:MF_00185};
GN Name=miaA {ECO:0000255|HAMAP-Rule:MF_00185};
GN OrderedLocusNames=trd_A0869;
OS Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2).
OG Plasmid Tros.
OC Bacteria; Chloroflexi; Thermomicrobiales; Thermomicrobiaceae;
OC Thermomicrobium.
OX NCBI_TaxID=309801;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27502 / DSM 5159 / P-2;
RX PubMed=19148287; DOI=10.1371/journal.pone.0004207;
RA Wu D., Raymond J., Wu M., Chatterji S., Ren Q., Graham J.E.,
RA Bryant D.A., Robb F., Colman A., Tallon L.J., Badger J.H., Madupu R.,
RA Ward N.L., Eisen J.A.;
RT "Complete genome sequence of the aerobic CO-oxidizing thermophile
RT Thermomicrobium roseum.";
RL PLoS ONE 4:E4207-E4207(2009).
CC -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC adenine at position 37 in tRNAs that read codons beginning with
CC uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC (i(6)A). {ECO:0000255|HAMAP-Rule:MF_00185}.
CC -!- CATALYTIC ACTIVITY: Dimethylallyl diphosphate + adenine(37) in
CC tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.
CC {ECO:0000255|HAMAP-Rule:MF_00185}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00185};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00185}.
CC -!- SIMILARITY: Belongs to the IPP transferase family.
CC {ECO:0000255|HAMAP-Rule:MF_00185}.
DR EMBL; CP001276; ACM06614.1; -; Genomic_DNA.
DR RefSeq; WP_012642601.1; NC_011961.1.
DR ProteinModelPortal; B9L505; -.
DR EnsemblBacteria; ACM06614; ACM06614; trd_A0869.
DR KEGG; tro:trd_A0869; -.
DR PATRIC; 23916777; VBITheRos91376_2723.
DR HOGENOM; HOG000039995; -.
DR KO; K00791; -.
DR OMA; HTRNYAK; -.
DR OrthoDB; POG091H00NK; -.
DR Proteomes; UP000000447; Plasmid Tros.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0052381; F:tRNA dimethylallyltransferase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-HAMAP.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00185; IPP_trans; 1.
DR InterPro; IPR018022; IPP_trans.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01715; IPPT; 1.
DR SUPFAM; SSF52540; SSF52540; 3.
DR TIGRFAMs; TIGR00174; miaA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Magnesium; Nucleotide-binding;
KW Plasmid; Reference proteome; Transferase; tRNA processing.
FT CHAIN 1 329 tRNA dimethylallyltransferase.
FT /FTId=PRO_0000377354.
FT NP_BIND 12 19 ATP. {ECO:0000255|HAMAP-Rule:MF_00185}.
FT REGION 14 19 Substrate binding. {ECO:0000255|HAMAP-
FT Rule:MF_00185}.
FT REGION 37 40 Interaction with substrate tRNA.
FT {ECO:0000255|HAMAP-Rule:MF_00185}.
FT SITE 103 103 Interaction with substrate tRNA.
FT {ECO:0000255|HAMAP-Rule:MF_00185}.
FT SITE 126 126 Interaction with substrate tRNA.
FT {ECO:0000255|HAMAP-Rule:MF_00185}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 7 307 ihamap:IPP_trans [T]
FT MYHIT 41 284 ipfam:IPPT [T]
SQ SEQUENCE 329 AA; 37580 MW; 4003240437EEE45C CRC64;
MNKRKPVVAL VGPTSVGKTA TAIELALTFG GEVVSADSRY LYRGMDIGTD KPTLEERRGV
PHHLIDILDP RDDYSLALFQ RDAQRAIEEI HARGRLPIVA GGTPLYLRAL LEGWRIPPAP
PNPELRAQLE LRARREGPEA LHRELQTVDP VAAARIPPEN VRRVIRALEI FLTTGRRMTE
LEGREAPPWR VLWLGLTMPR DELYRRIDER VDRQVARGLV EEVQRLLEQG VPPDAPAMTA
LGYRQIVAFL TGQLSLEEAI QRIKYDTHRY ARHQLTWLRR MKQVEWYDVT QPGWYEQLRE
RVERSLREES DEGDVAVHQS GGGKEAPRA
//
|
