ID MG179_SCHPO Reviewed; 335 AA.
AC Q9P6N1;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 30-NOV-2016, entry version 104.
DE RecName: Full=Autophagy-related protein 21;
DE AltName: Full=Meiotically up-regulated gene 179 protein;
GN Name=mug179; Synonyms=atg18b; ORFNames=SPAC823.16c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales;
OC Schizosaccharomycetaceae; Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION IN SPORULATION.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L.,
RA San-Segundo P., Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes
RT required for critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the
RT fission yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=23950735; DOI=10.1371/journal.pgen.1003715;
RA Sun L.L., Li M., Suo F., Liu X.M., Shen E.Z., Yang B., Dong M.Q.,
RA He W.Z., Du L.L.;
RT "Global analysis of fission yeast mating genes reveals new autophagy
RT factors.";
RL PLoS Genet. 9:E1003715-E1003715(2013).
CC -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt)
CC vesicles formation and autophagy (By similarity). Has a role in
CC sporulation. {ECO:0000250, ECO:0000269|PubMed:16303567}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus, Golgi stack
CC membrane; Peripheral membrane protein. Vacuole membrane;
CC Peripheral membrane protein. Preautophagosomal structure membrane;
CC Peripheral membrane protein.
CC -!- DISRUPTION PHENOTYPE: Impairs atg8-processing.
CC {ECO:0000269|PubMed:23950735}.
CC -!- SIMILARITY: Belongs to the WD repeat SVP1 family. {ECO:0000305}.
CC -!- SIMILARITY: Contains 2 WD repeats. {ECO:0000305}.
DR EMBL; CU329670; CAB90161.1; -; Genomic_DNA.
DR RefSeq; NP_593843.1; NM_001019272.2.
DR ProteinModelPortal; Q9P6N1; -.
DR BioGrid; 279722; 1.
DR MINT; MINT-4704393; -.
DR PRIDE; Q9P6N1; -.
DR EnsemblFungi; SPAC823.16c.1; SPAC823.16c.1:pep; SPAC823.16c.
DR GeneID; 2543297; -.
DR KEGG; spo:SPAC823.16c; -.
DR EuPathDB; FungiDB:SPAC823.16c; -.
DR PomBase; SPAC823.16c; -.
DR InParanoid; Q9P6N1; -.
DR OMA; NGASICE; -.
DR OrthoDB; EOG092C3UKJ; -.
DR PhylomeDB; Q9P6N1; -.
DR Reactome; R-SPO-1632852; Macroautophagy.
DR PRO; PR:Q9P6N1; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; ISO:PomBase.
DR GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0000329; C:fungal-type vacuole membrane; ISO:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:PomBase.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000407; C:pre-autophagosomal structure; IDA:PomBase.
DR GO; GO:0034045; C:pre-autophagosomal structure membrane; IBA:GO_Central.
DR GO; GO:0043234; C:protein complex; NAS:PomBase.
DR GO; GO:0035091; F:phosphatidylinositol binding; ISO:PomBase.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0006914; P:autophagy; IMP:PomBase.
DR GO; GO:0016236; P:macroautophagy; IMP:PomBase.
DR GO; GO:0000422; P:mitophagy; IBA:GO_Central.
DR GO; GO:0044804; P:nucleophagy; IBA:GO_Central.
DR GO; GO:0006497; P:protein lipidation; IBA:GO_Central.
DR GO; GO:0034497; P:protein localization to pre-autophagosomal structure; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; ISO:PomBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR017986; WD40_repeat_dom.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 2.
PE 1: Evidence at protein level;
KW Autophagy; Complete proteome; Cytoplasm; Golgi apparatus; Membrane;
KW Protein transport; Reference proteome; Repeat; Sporulation; Transport;
KW Vacuole; WD repeat.
FT CHAIN 1 335 Autophagy-related protein 21.
FT /FTId=PRO_0000050881.
FT REPEAT 165 205 WD 1.
FT REPEAT 210 249 WD 2.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 156 196 ismart:WD40 [T]
FT MYHIT 199 240 ismart:WD40 [T]
SQ SEQUENCE 335 AA; 37108 MW; BBACA9C3E4915705 CRC64;
MPSIILYCSW NQDRGFLSIG SENGYQVYRS NPFTLCFSKK ANGASICEML YESSLLAFVN
ISPESTRLLK LVDIKRDIVL CRIFYPSPVL SVRFTWNRLV VLIKGSIYVY NLKNMELINT
LNTSKGNVIA FAVHENYVAY NSPTNPGDIY LASLDTAIPV TLIHCHSSAV QVVDFHPRGH
LIATASAKGT VIRVITTSDG ELVTELRRGY IPASIVSISF HPVEPFLACA SENGTIHVFK
ISKQPSDPNS SPTSSVTVSS SWSKYLTSNV AKVWDTRKEF ATAKIPEASF YGKIIFSSSG
PHIQVASYSG HYYRFAVNLK NGGNCALLER YIFDD
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