Entry sw:MEPA_PSEPU

ID   MEPA_PSEPU              Reviewed;         384 AA.
AC   P0C069;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   05-OCT-2016, entry version 43.
DE   RecName: Full=Multidrug/solvent efflux pump periplasmic linker protein MepA;
DE   Flags: Precursor;
GN   Name=mepA;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=KT2442-TOL;
RX   PubMed=9827328; DOI=10.1007/s007920050084;
RA   Fukumori F., Hirayama H., Takami H., Inoue A., Horikoshi K.;
RT   "Isolation and transposon mutagenesis of a Pseudomonas putida KT2442
RT   toluene-resistant variant: involvement of an efflux system in solvent
RT   resistance.";
RL   Extremophiles 2:395-400(1998).
CC   -!- FUNCTION: The periplasmic linker protein component of an organic
CC       solvent and antibiotic efflux pump; confers resistance to toluene,
CC       hexane, p-xylene, ampicillin, penicillin G, erythromycin,
CC       novobiocin and tetracycline.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-
CC       anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP)
CC       (TC 8.A.1) family. {ECO:0000305}.
CC   -!- CAUTION: There are 4 nearly identical operons in various strains
CC       of P.putida. This one and the ttgABC operon of strain DOT-T1E
CC       function in solvent and antibiotic efflux; however in strain S12
CC       the arpABC operon functions only in antibiotic efflux. This may be
CC       due to different protein expression levels. In strain KT2440 the
CC       equivalent operon does not seem to function in toluene efflux.
CC       {ECO:0000305}.
DR   RefSeq; WP_010952494.1; NZ_LFYQ01000017.1.
DR   ProteinModelPortal; P0C069; -.
DR   STRING; 160488.PP_1386; -.
DR   eggNOG; ENOG4105C1P; Bacteria.
DR   eggNOG; ENOG410XNVN; LUCA.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; HlyD; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane;
KW   Coiled coil; Lipoprotein; Membrane; Palmitate; Signal; Transport.
FT   SIGNAL        1     22       {ECO:0000255|PROSITE-ProRule:PRU00303}.
FT   CHAIN        23    384       Multidrug/solvent efflux pump periplasmic
FT                                linker protein MepA.
FT                                /FTId=PRO_0000018711.
FT   COILED      115    155       {ECO:0000255}.
FT   LIPID        23     23       N-palmitoyl cysteine.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00303}.
FT   LIPID        23     23       S-diacylglycerol cysteine.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT        60    277       ipfam:HlyD_D23 [T]
FT   MYHIT        34    344       ipfam:HlyD [T]
SQ   SEQUENCE   384 AA;  41250 MW;  16BB3FA87E3C16CA CRC64;
     MQFKPAVTAL VSAVALATLL SGCKKEEAAP AAQAPQVGVV TIQPQAFTLT SELPGRTSAY
     RVAEVRPQVN GIILKRLFKE GSEVKEGQQL YQIDPAVYEA TLANAKANLL ATRSLAERYK
     QLIDEQAVSK QEYDDANAKR LQAEASLKSA QIDLRYTKVL APISGRIGRS SFTEGALVSN
     GQTDAMATIQ QLDPIYVDVT QSTAELLKLR RDLESGQLQK AGDNAASVQL VLEDGSLFKQ
     EGRLEFSEVA VDETTGSVTL RALFPNPDHT LLPGMFVHAR LKAGVNANAI LAPQQGVTRD
     LKGAPTALVV NQENKVELRQ LKASRTLGSD WLIEEGLNPG DRLITEGLQY VRPGVEVKVS
     DATNVKKPAG PDQANAAKAD AKAE
//