Legends: 1, CONFLICT K -> N (in Ref. 1; AAC36870). {ECO:0000305}; 2, CONFLICT D -> A (in Ref. 1; AAC36870). {ECO:0000305}; 3, TOPO_DOM Cytoplasmic. {ECO:0000255}; 4, TRANSMEM Helical. {ECO:0000255|PROSITE- ProRule:PRU00441}; 5, TOPO_DOM Periplasmic. {ECO:0000255}; 6, ABC transmembrane type-1. {ECO:0000255|PROSITE-ProRule:PRU00441}; 7, ABC transporter. {ECO:0000255|PROSITE- ProRule:PRU00434}; 8, NP_BIND ATP. {ECO:0000255|PROSITE- ProRule:PRU00434}; 9, CONFLICT NE -> KQ (in Ref. 1; AAC36870). {ECO:0000305}; 10, CONFLICT LQ -> FT (in Ref. 1; AAC36870). {ECO:0000305}; 11, CONFLICT SALR -> TRG (in Ref. 1; AAC36870). {ECO:0000305}; 12, CONFLICT EL -> DV (in Ref. 1; AAC36870). {ECO:0000305}; 13, CONFLICT AAQGRYWQMYQLQLAGEELAASVREEESLSA -> RPRDVL ADVSTATCGRRAGSQRA (in Ref. 1; AAC36870). {ECO:0000305}; 14, ipat:ABC_TRANSPORTER_1 [T].
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ID MDLB_ECOLI Reviewed; 593 AA.
AC P0AAG5; P30751; P75706; P77117; Q2MBX7;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 02-NOV-2016, entry version 97.
DE RecName: Full=Multidrug resistance-like ATP-binding protein MdlB;
DE EC=3.6.3.44;
GN Name=mdlB; Synonyms=mdl; OrderedLocusNames=b0449, JW5061;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=TAP90 / ATCC 47037;
RX PubMed=7904973; DOI=10.1016/0378-1119(93)90470-N;
RA Allikmets R., Gerrard B.C., Court D., Dean M.C.;
RT "Cloning and organization of the abc and mdl genes of Escherichia
RT coli: relationship to eukaryotic multidrug resistance.";
RL Gene 136:231-236(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- CATALYTIC ACTIVITY: ATP + H(2)O + xenobiotic(In) = ADP + phosphate
CC + xenobiotic(Out).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Drug
CC exporter-2 (TC 3.A.1.117) family. {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 ABC transmembrane type-1 domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- SIMILARITY: Contains 1 ABC transporter domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00434}.
CC -!- CAUTION: Was originally proposed to be fused with MdlA.
CC {ECO:0000305|PubMed:7904973}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB40205.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAC36870.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR EMBL; L08627; AAC36870.1; ALT_INIT; Unassigned_DNA.
DR EMBL; U82664; AAB40205.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73552.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76229.1; -; Genomic_DNA.
DR PIR; A64775; A64775.
DR RefSeq; NP_414983.1; NC_000913.3.
DR RefSeq; WP_001256201.1; NZ_LN832404.1.
DR ProteinModelPortal; P0AAG5; -.
DR SMR; P0AAG5; -.
DR BioGrid; 4261405; 173.
DR STRING; 511145.b0449; -.
DR TCDB; 3.A.1.106.13; the atp-binding cassette (abc) superfamily.
DR PaxDb; P0AAG5; -.
DR PRIDE; P0AAG5; -.
DR EnsemblBacteria; AAC73552; AAC73552; b0449.
DR EnsemblBacteria; BAE76229; BAE76229; BAE76229.
DR GeneID; 945088; -.
DR KEGG; ecj:JW5061; -.
DR KEGG; eco:b0449; -.
DR PATRIC; 32116051; VBIEscCol129921_0467.
DR EchoBASE; EB4117; -.
DR EcoGene; EG14374; mdlB.
DR eggNOG; ENOG4105BZ1; Bacteria.
DR eggNOG; COG1132; LUCA.
DR InParanoid; P0AAG5; -.
DR KO; K18890; -.
DR OMA; IVMIIYQ; -.
DR PhylomeDB; P0AAG5; -.
DR BioCyc; EcoCyc:MDLB-MONOMER; -.
DR BioCyc; ECOL316407:JW5061-MONOMER; -.
DR PRO; PR:P0AAG5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0034040; F:lipid-transporting ATPase activity; IBA:GO_Central.
DR GO; GO:0008559; F:xenobiotic-transporting ATPase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR003439; ABC_transporter-like.
DR InterPro; IPR017871; ABC_transporter_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00664; ABC_membrane; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF90123; SSF90123; 1.
DR PROSITE; PS50929; ABC_TM1F; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell inner membrane; Cell membrane; Complete proteome;
KW Hydrolase; Membrane; Nucleotide-binding; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 593 Multidrug resistance-like ATP-binding
FT protein MdlB.
FT /FTId=PRO_0000092496.
FT TOPO_DOM 1 25 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 26 46 Helical. {ECO:0000255|PROSITE-
FT ProRule:PRU00441}.
FT TOPO_DOM 47 62 Periplasmic. {ECO:0000255}.
FT TRANSMEM 63 83 Helical. {ECO:0000255|PROSITE-
FT ProRule:PRU00441}.
FT TOPO_DOM 84 140 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 141 161 Helical. {ECO:0000255|PROSITE-
FT ProRule:PRU00441}.
FT TOPO_DOM 162 164 Periplasmic. {ECO:0000255}.
FT TRANSMEM 165 185 Helical. {ECO:0000255|PROSITE-
FT ProRule:PRU00441}.
FT TOPO_DOM 186 254 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 255 275 Helical. {ECO:0000255|PROSITE-
FT ProRule:PRU00441}.
FT TOPO_DOM 276 278 Periplasmic. {ECO:0000255}.
FT TRANSMEM 279 299 Helical. {ECO:0000255|PROSITE-
FT ProRule:PRU00441}.
FT TOPO_DOM 300 593 Cytoplasmic. {ECO:0000255}.
FT DOMAIN 25 310 ABC transmembrane type-1.
FT {ECO:0000255|PROSITE-ProRule:PRU00441}.
FT DOMAIN 341 574 ABC transporter. {ECO:0000255|PROSITE-
FT ProRule:PRU00434}.
FT NP_BIND 374 381 ATP. {ECO:0000255|PROSITE-
FT ProRule:PRU00434}.
FT CONFLICT 208 209 NE -> KQ (in Ref. 1; AAC36870).
FT {ECO:0000305}.
FT CONFLICT 336 337 LQ -> FT (in Ref. 1; AAC36870).
FT {ECO:0000305}.
FT CONFLICT 358 358 K -> N (in Ref. 1; AAC36870).
FT {ECO:0000305}.
FT CONFLICT 411 414 SALR -> TRG (in Ref. 1; AAC36870).
FT {ECO:0000305}.
FT CONFLICT 457 458 EL -> DV (in Ref. 1; AAC36870).
FT {ECO:0000305}.
FT CONFLICT 542 542 D -> A (in Ref. 1; AAC36870).
FT {ECO:0000305}.
FT CONFLICT 563 593 AAQGRYWQMYQLQLAGEELAASVREEESLSA -> RPRDVL
FT ADVSTATCGRRAGSQRA (in Ref. 1; AAC36870).
FT {ECO:0000305}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 366 557 ismart:AAA [T]
FT MYHIT 27 310 iprf:ABC_TM1F [T]
FT MYHIT 477 491 ipat:ABC_TRANSPORTER_1 [T]
FT MYHIT 357 505 ipfam:ABC_tran [T]
FT MYHIT 341 574 iprf:ABC_TRANSPORTER_2 [T]
FT MYHIT 26 295 ipfam:ABC_membrane [T]
SQ SEQUENCE 593 AA; 65222 MW; CF1EE70009EB05D8 CRC64;
MRSFSQLWPT LKRLLAYGSP WRKPLGIAVL MMWVAAAAEV SGPLLISYFI DNMVAKNNLP
LKVVAGLAAA YVGLQLFAAG LHYAQSLLFN RAAVGVVQQL RTDVMDAALR QPLSEFDTQP
VGQVISRVTN DTEVIRDLYV TVVATVLRSA ALVGAMLVAM FSLDWRMALV AIMIFPVVLV
VMVIYQRYST PIVRRVRAYL ADINDGFNEI INGMSVIQQF RQQARFGERM GEASRSHYMA
RMQTLRLDGF LLRPLLSLFS SLILCGLLML FGFSASGTIE VGVLYAFISY LGRLNEPLIE
LTTQQAMLQQ AVVAGERVFE LMDGPRQQYG NDDRPLQSGT IEVDNVSFAY RDDNLVLKNI
NLSVPSRNFV ALVGHTGSGK STLASLLMGY YPLTEGEIRL DGRPLSSLSH SALRQGVAMV
QQDPVVLADT FLANVTLGRD ISEERVWQAL ETVQLAELAR SMSDGIYTPL GEQGNNLSVG
QKQLLALARV LVETPQILIL DEATASIDSG TEQAIQHALA AVREHTTLVV IAHRLSTIVD
ADTILVLHRG QAVEQGTHQQ LLAAQGRYWQ MYQLQLAGEE LAASVREEES LSA
//
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