MyHits has reached its end of life and no longer provides data or services. Thank you for your support and trust for more than 23 years!
However, the webserver will remain online in its present form at least until end of March 2025.
To ensure the future of MyHits, we would be happy if a person or community would take over the resource or parts of it. Interested? Please contact us (myhits [at] sib.swiss).
Pagni M, Ioannidis V, Cerutti L, Zahn-Zabal M, Jongeneel CV, Hau J, Martin O, Kuznetsov D, Falquet L.
MyHits: improvements to an interactive resource for analyzing protein sequences.
Nucleic Acids Res. 2007 Jul; 35(Web Server issue):W433-7
However, the webserver will remain online in its present form at least until end of March 2025.
To ensure the future of MyHits, we would be happy if a person or community would take over the resource or parts of it. Interested? Please contact us (myhits [at] sib.swiss).
Pagni M, Ioannidis V, Cerutti L, Zahn-Zabal M, Jongeneel CV, Hau J, Martin O, Kuznetsov D, Falquet L.
MyHits: improvements to an interactive resource for analyzing protein sequences.
Nucleic Acids Res. 2007 Jul; 35(Web Server issue):W433-7
- MyHits
| Description | RecName: Full=Protein lap1; AltName: Full=Lethal protein 413; |
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| MyHits synonyms | LAP1_CAEEL , O61967 , Q6A580 , Q9NFN7 , 2CE6478B5039F2DE |
 Legends: 1, VAR_SEQ R -> RFIDAPAS (in isoform a). {ECO:0000303|PubMed:10878806}; 2, MUTAGEN P->L: Abolishes membrane localization. {ECO:0000269|PubMed:10878806, ECO:0000269|PubMed:14578922}; 3, REPEAT LRR 1; 4, REPEAT LRR 2; 5, REPEAT LRR 3; 6, REPEAT LRR 4; 7, REPEAT LRR 5; 8, REPEAT LRR 6; 9, REPEAT LRR 7; 10, REPEAT LRR 8; 11, REPEAT LRR 9; 12, REPEAT LRR 10; 13, REPEAT LRR 11; 14, REPEAT LRR 12; 15, REPEAT LRR 13; 16, REPEAT LRR 14; 17, REPEAT LRR 15; 18, REPEAT LRR 16; 19, PDZ. {ECO:0000255|PROSITE- ProRule:PRU00143}; 20, VAR_SEQ EPSLNGSSHQLNHFDAGSPDSTMFVTSSTPVYAS -> VSR PCEY (in isoform a). {ECO:0000303|PubMed:10878806}; 21, ismart:LRR_TYP [T]; 22, iprf:LRR [T]; 23, ipfam:LRR_8 [T]; 24, ismart:PDZ [T].
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ID LAP1_CAEEL Reviewed; 699 AA.
AC O61967; Q6A580; Q9NFN7;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 3.
DT 30-NOV-2016, entry version 136.
DE RecName: Full=Protein lap1;
DE AltName: Full=Lethal protein 413;
GN Name=let-413; ORFNames=F26D11.11;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF PRO-305, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=Bristol N2 {ECO:0000269|PubMed:10878806};
RX PubMed=10878806; DOI=10.1038/35017046;
RA Legouis R., Gansmuller A., Sookhareea S., Bosher J.M., Baillie D.L.,
RA Labouesse M.;
RT "LET-413 is a basolateral protein required for the assembly of
RT adherens junctions in Caenorhabditis elegans.";
RL Nat. Cell Biol. 2:415-422(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE
RP SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for
RT investigating biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305, ECO:0000312|EMBL:CAB91651.1}
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF PRO-305.
RX PubMed=14578922; DOI=10.1038/sj.embor.7400006;
RA Legouis R., Jaulin-Bastard F., Schott S., Navarro C., Borg J.-P.,
RA Labouesse M.;
RT "Basolateral targeting by leucine-rich repeat domains in epithelial
RT cells.";
RL EMBO Rep. 4:1096-1102(2003).
RN [4] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15063180; DOI=10.1016/j.ydbio.2004.01.003;
RA Bossinger O., Fukushige T., Claeys M., Borgonie G., McGhee J.D.;
RT "The apical disposition of the Caenorhabditis elegans intestinal
RT terminal web is maintained by LET-413.";
RL Dev. Biol. 268:448-456(2004).
CC -!- FUNCTION: Critical role in assembling adherens junctions; adapter
CC protein involved in polarizing protein trafficking in epithelial
CC cells. Necessary to maintain, not establish, the entire terminal
CC web (organelle-depleted, intermediate filament-rich layer of
CC cytoplasm that underlies the apical microvilli of polarized
CC epithelial cells) or brush border assembly at the apical surface
CC gut cells. Required for correct localization of ifb-2 intermediate
CC filaments in the terminal web. {ECO:0000269|PubMed:10878806,
CC ECO:0000269|PubMed:15063180}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000269|PubMed:10878806, ECO:0000269|PubMed:14578922};
CC Peripheral membrane protein {ECO:0000269|PubMed:10878806,
CC ECO:0000269|PubMed:14578922}. Note=Basolateral membrane of
CC epithelial cells.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b;
CC IsoId=O61967-1; Sequence=Displayed;
CC Note=No experimental confirmation available.;
CC Name=a;
CC IsoId=O61967-2; Sequence=VSP_015673, VSP_015674;
CC -!- TISSUE SPECIFICITY: All embryonic epithelial cells. Terminal web
CC of the adult intestine. {ECO:0000269|PubMed:10878806,
CC ECO:0000269|PubMed:15063180}.
CC -!- DISRUPTION PHENOTYPE: Worms display a severely affected epithelial
CC integrity, leading to abnormal morphogenesis of the pharynx,
CC intestine and embryo. {ECO:0000269|PubMed:10878806}.
CC -!- SIMILARITY: Belongs to the LAP (LRR and PDZ) protein family.
CC {ECO:0000305}.
CC -!- SIMILARITY: Contains 16 LRR (leucine-rich) repeats. {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 PDZ (DHR) domain. {ECO:0000255|PROSITE-
CC ProRule:PRU00143}.
DR EMBL; AJ276590; CAB91651.1; -; mRNA.
DR EMBL; FO081227; CCD70053.1; -; Genomic_DNA.
DR EMBL; FO081227; CCD70054.1; -; Genomic_DNA.
DR PIR; T33295; T33295.
DR RefSeq; NP_001023850.1; NM_001028679.3. [O61967-2]
DR RefSeq; NP_001023851.1; NM_001028680.3. [O61967-1]
DR UniGene; Cel.6189; -.
DR ProteinModelPortal; O61967; -.
DR BioGrid; 44213; 10.
DR DIP; DIP-25349N; -.
DR IntAct; O61967; 7.
DR MINT; MINT-1058604; -.
DR STRING; 6239.F26D11.11b; -.
DR iPTMnet; O61967; -.
DR EPD; O61967; -.
DR PaxDb; O61967; -.
DR PeptideAtlas; O61967; -.
DR EnsemblMetazoa; F26D11.11b; F26D11.11b; WBGene00002632. [O61967-1]
DR GeneID; 179171; -.
DR KEGG; cel:CELE_F26D11.11; -.
DR UCSC; F26D11.11a; c. elegans. [O61967-1]
DR CTD; 179171; -.
DR WormBase; F26D11.11a; CE29778; WBGene00002632; let-413. [O61967-2]
DR WormBase; F26D11.11b; CE37008; WBGene00002632; let-413. [O61967-1]
DR eggNOG; KOG0619; Eukaryota.
DR eggNOG; KOG3528; Eukaryota.
DR eggNOG; COG4886; LUCA.
DR GeneTree; ENSGT00840000129686; -.
DR HOGENOM; HOG000116557; -.
DR InParanoid; O61967; -.
DR OMA; FERHNTP; -.
DR OrthoDB; EOG091G01EF; -.
DR PhylomeDB; O61967; -.
DR Reactome; R-CEL-6798695; Neutrophil degranulation.
DR PRO; PR:O61967; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00002632; -.
DR GO; GO:0005912; C:adherens junction; IDA:UniProtKB.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:WormBase.
DR GO; GO:0030029; P:actin filament-based process; IMP:WormBase.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0007043; P:cell-cell junction assembly; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0048557; P:embryonic digestive tract morphogenesis; IMP:WormBase.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; IEP:UniProtKB.
DR GO; GO:0045199; P:maintenance of epithelial cell apical/basal polarity; IMP:WormBase.
DR GO; GO:0015031; P:protein transport; IEP:UniProtKB.
DR GO; GO:0045108; P:regulation of intermediate filament polymerization or depolymerization; IMP:UniProtKB.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR032675; L_dom-like.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR001478; PDZ.
DR Pfam; PF13855; LRR_8; 3.
DR Pfam; PF00595; PDZ; 1.
DR SMART; SM00369; LRR_TYP; 12.
DR SMART; SM00228; PDZ; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR PROSITE; PS51450; LRR; 14.
DR PROSITE; PS50106; PDZ; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell adhesion; Cell membrane; Complete proteome;
KW Leucine-rich repeat; Membrane; Reference proteome; Repeat.
FT CHAIN 1 699 Protein lap1.
FT /FTId=PRO_0000188305.
FT REPEAT 37 59 LRR 1.
FT REPEAT 60 81 LRR 2.
FT REPEAT 83 104 LRR 3.
FT REPEAT 106 127 LRR 4.
FT REPEAT 129 150 LRR 5.
FT REPEAT 152 174 LRR 6.
FT REPEAT 175 196 LRR 7.
FT REPEAT 198 219 LRR 8.
FT REPEAT 221 242 LRR 9.
FT REPEAT 244 265 LRR 10.
FT REPEAT 267 288 LRR 11.
FT REPEAT 290 311 LRR 12.
FT REPEAT 313 334 LRR 13.
FT REPEAT 336 357 LRR 14.
FT REPEAT 359 380 LRR 15.
FT REPEAT 382 403 LRR 16.
FT DOMAIN 584 665 PDZ. {ECO:0000255|PROSITE-
FT ProRule:PRU00143}.
FT VAR_SEQ 531 531 R -> RFIDAPAS (in isoform a).
FT {ECO:0000303|PubMed:10878806}.
FT /FTId=VSP_015673.
FT VAR_SEQ 666 699 EPSLNGSSHQLNHFDAGSPDSTMFVTSSTPVYAS -> VSR
FT PCEY (in isoform a).
FT {ECO:0000303|PubMed:10878806}.
FT /FTId=VSP_015674.
FT MUTAGEN 305 305 P->L: Abolishes membrane localization.
FT {ECO:0000269|PubMed:10878806,
FT ECO:0000269|PubMed:14578922}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 59 80 ismart:LRR_TYP [T]
FT MYHIT 568 658 iprf:PDZ [T]
FT MYHIT 265 288 ismart:LRR_TYP [T]
FT MYHIT 221 242 iprf:LRR [T]
FT MYHIT 59 116 ipfam:LRR_8 [T]
FT MYHIT 81 103 ismart:LRR_TYP [T]
FT MYHIT 150 170 ismart:LRR_TYP [T]
FT MYHIT 336 357 iprf:LRR [T]
FT MYHIT 175 196 iprf:LRR [T]
FT MYHIT 196 219 ismart:LRR_TYP [T]
FT MYHIT 244 265 iprf:LRR [T]
FT MYHIT 569 653 ipfam:PDZ [T]
FT MYHIT 174 232 ipfam:LRR_8 [T]
FT MYHIT 577 657 ismart:PDZ [T]
FT MYHIT 289 310 ismart:LRR_TYP [T]
FT MYHIT 312 370 ipfam:LRR_8 [T]
FT MYHIT 83 104 iprf:LRR [T]
FT MYHIT 290 311 iprf:LRR [T]
FT MYHIT 60 81 iprf:LRR [T]
FT MYHIT 104 127 ismart:LRR_TYP [T]
FT MYHIT 106 127 iprf:LRR [T]
FT MYHIT 311 334 ismart:LRR_TYP [T]
FT MYHIT 313 334 iprf:LRR [T]
FT MYHIT 357 377 ismart:LRR_TYP [T]
FT MYHIT 198 219 iprf:LRR [T]
FT MYHIT 242 264 ismart:LRR_TYP [T]
FT MYHIT 37 59 iprf:LRR [T]
FT MYHIT 35 58 ismart:LRR_TYP [T]
FT MYHIT 173 195 ismart:LRR_TYP [T]
FT MYHIT 152 174 iprf:LRR [T]
FT MYHIT 382 403 iprf:LRR [T]
FT MYHIT 359 380 iprf:LRR [T]
SQ SEQUENCE 699 AA; 77350 MW; 2CE6478B5039F2DE CRC64;
MPAFFCLPMA CQRQVDSIDR SQSNLQAIPS DIFRFRKLED LNLTMNNIKE LDHRLFSLRH
LRILDVSDNE LAVLPAEIGN LTQLIELNLN RNSIAKLPDT MQNCKLLTTL NLSSNPFTRL
PETICECSSI TILSLNETSL TLLPSNIGSL TNLRVLEARD NLLRTIPLSI VELRKLEELD
LGQNELEALP AEIGKLTSLR EFYVDINSLT SLPDSISGCR MLDQLDVSEN QIIRLPENLG
RMPNLTDLNI SINEIIELPS SFGELKRLQM LKADRNSLHN LTSEIGKCQS LTELYLGQNF
LTDLPDTIGD LRQLTTLNVD CNNLSDIPDT IGNCKSLTVL SLRQNILTEL PMTIGKCENL
TVLDVASNKL PHLPFTVKVL YKLQALWLSE NQTQSILKLS ETRDDRKGIK VVTCYLLPQV
DAIDGEGRSG SAQHNTDRGA FLGGPKVHFH DQADTTFEEN KEAEIHLGNF ERHNTPHPKT
PKHKKGSIDG HMLPHEIDQP RQLSLVSNHR TSTSSFGESS NSINRDLADI RAQNGVREAT
LSPEREERMA TSLSSLSNLA AGTQNMHTIR IQKDDTGKLG LSFAGGTSND PAPNSNGDSG
LFVTKVTPGS AAYRCGLREG DKLIRANDVN MINASQDNAM EAIKKRETVE LVVLRRSPSP
VSRTSEPSLN GSSHQLNHFD AGSPDSTMFV TSSTPVYAS
//
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