Legends: 1, N-acetylmethionine. {ECO:0000250|UniProtKB:Q9UG01}; 2, Omega-N-methylarginine. {ECO:0000250|UniProtKB:Q6VH22}; 3, CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1). {ECO:0000250|UniProtKB:Q9UG01}; 4, REPEAT WD 1; 5, REPEAT WD 2; 6, REPEAT WD 3; 7, REPEAT WD 4; 8, REPEAT WD 5; 9, REPEAT WD 6; 10, REPEAT WD 7; 11, REPEAT WD 8; 12, REPEAT WD 9; 13, REPEAT TPR 1; 14, REPEAT TPR 2; 15, REPEAT TPR 3; 16, REPEAT TPR 4; 17, REPEAT TPR 5; 18, REPEAT TPR 6; 19, REPEAT TPR 7; 20, REPEAT TPR 8; 21, REPEAT TPR 9; 22, REPEAT TPR 10; 23, REPEAT TPR 11; 24, REPEAT TPR 12; 25, REPEAT TPR 13; 26, REPEAT TPR 14; 27, ismart:WD40 [T]; 28, ipfam:WD40 [T].
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ID IF172_RAT Reviewed; 1749 AA.
AC Q9JKU3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 18-JAN-2017, entry version 104.
DE RecName: Full=Intraflagellar transport protein 172 homolog;
DE AltName: Full=Selective LIM-binding factor;
GN Name=Ift172; Synonyms=Slb;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC Muroidea; Muridae; Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, POSSIBLE INTERACTION
RP WITH LHX3 AND LHX4, AND TISSUE SPECIFICITY.
RX PubMed=10788441; DOI=10.1074/jbc.275.18.13336;
RA Howard P.W., Maurer R.A.;
RT "Identification of a conserved protein that interacts with specific
RT LIM homeodomain transcription factors.";
RL J. Biol. Chem. 275:13336-13342(2000).
CC -!- FUNCTION: Required for the maintenance and formation of cilia.
CC Plays an indirect role in hedgehog (Hh) signaling, cilia being
CC required for all activity of the hedgehog pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with IFT88 (By similarity). Interacts with
CC RABL2/RABL2A; binds preferentially to GDP-bound RABL2 (By
CC similarity). May interact with LHX3 and LHX4; the relevance of
CC such interaction is however unclear in vivo. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000250}.
CC Nucleus {ECO:0000269|PubMed:10788441}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the testis and pituitary
CC cells. {ECO:0000269|PubMed:10788441}.
CC -!- SIMILARITY: Belongs to the IFT172 family. {ECO:0000305}.
CC -!- SIMILARITY: Contains 14 TPR repeats. {ECO:0000305}.
CC -!- SIMILARITY: Contains 9 WD repeats. {ECO:0000305}.
DR EMBL; AF226993; AAF68274.1; -; mRNA.
DR RefSeq; NP_446244.1; NM_053792.1.
DR UniGene; Rn.171849; -.
DR ProteinModelPortal; Q9JKU3; -.
DR iPTMnet; Q9JKU3; -.
DR PhosphoSitePlus; Q9JKU3; -.
DR PRIDE; Q9JKU3; -.
DR GeneID; 116475; -.
DR KEGG; rno:116475; -.
DR CTD; 26160; -.
DR RGD; 620744; Ift172.
DR HOVERGEN; HBG098449; -.
DR InParanoid; Q9JKU3; -.
DR KO; K19676; -.
DR PhylomeDB; Q9JKU3; -.
DR PRO; PR:Q9JKU3; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005930; C:axoneme; IBA:GO_Central.
DR GO; GO:0036064; C:ciliary basal body; IBA:GO_Central.
DR GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0008134; F:transcription factor binding; IDA:RGD.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0007275; P:multicellular organism development; IEA:UniProtKB-KW.
DR GO; GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:RGD.
DR Gene3D; 2.120.10.30; -; 2.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR017986; WD40_repeat_dom.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell projection; Cilium; Complete proteome;
KW Developmental protein; Isopeptide bond; Methylation; Nucleus;
KW Reference proteome; Repeat; TPR repeat; Ubl conjugation; WD repeat.
FT CHAIN 1 1749 Intraflagellar transport protein 172
FT homolog.
FT /FTId=PRO_0000328943.
FT REPEAT 14 53 WD 1.
FT REPEAT 64 103 WD 2.
FT REPEAT 110 148 WD 3.
FT REPEAT 150 191 WD 4.
FT REPEAT 195 233 WD 5.
FT REPEAT 238 278 WD 6.
FT REPEAT 284 323 WD 7.
FT REPEAT 483 520 WD 8.
FT REPEAT 521 559 WD 9.
FT REPEAT 593 624 TPR 1.
FT REPEAT 692 725 TPR 2.
FT REPEAT 809 842 TPR 3.
FT REPEAT 854 887 TPR 4.
FT REPEAT 912 945 TPR 5.
FT REPEAT 947 970 TPR 6.
FT REPEAT 971 1004 TPR 7.
FT REPEAT 1042 1075 TPR 8.
FT REPEAT 1142 1175 TPR 9.
FT REPEAT 1276 1309 TPR 10.
FT REPEAT 1345 1378 TPR 11.
FT REPEAT 1411 1445 TPR 12.
FT REPEAT 1447 1477 TPR 13.
FT REPEAT 1574 1607 TPR 14.
FT MOD_RES 1 1 N-acetylmethionine.
FT {ECO:0000250|UniProtKB:Q9UG01}.
FT MOD_RES 672 672 Omega-N-methylarginine.
FT {ECO:0000250|UniProtKB:Q6VH22}.
FT CROSSLNK 4 4 Glycyl lysine isopeptide (Lys-Gly)
FT (interchain with G-Cter in SUMO1).
FT {ECO:0000250|UniProtKB:Q9UG01}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 186 223 ismart:WD40 [T]
FT MYHIT 2 44 ismart:WD40 [T]
FT MYHIT 225 267 ismart:WD40 [T]
FT MYHIT 141 180 ismart:WD40 [T]
FT MYHIT 55 94 ismart:WD40 [T]
FT MYHIT 15 44 ipfam:WD40 [T]
FT MYHIT 102 139 ismart:WD40 [T]
FT MYHIT 279 314 ismart:WD40 [T]
SQ SEQUENCE 1749 AA; 197603 MW; B72C79207F5C0AA5 CRC64;
MQLKHLRTLL SPQDGAAKVT CMAWSQNNAK FAVCTVDRVV LLYDEHGERR DKFSTKPADM
KYGRKSYMVK GMAFSPDSTK IAIGQTDNII YVYKIGEDWG DKKVICNKFI QTSAVTCLQW
PAEYIIVFGL AEGKVRLANT KTNKSSTIYG TDSYVVALTT NCSGKGILSG HADGTIVRYF
FDDEGSGESQ GKLVNHPCPP YALAWATNSI VAAGCDRRIV AYGKEGHVLQ TFDYSRDPQE
REFTTAAASP GGQSVVLGSY DRLRVFNWSP RRSIWEEAKP KEIANLYTVT ALAWKRDGSR
LCAGTLCGGV EQFDCCLRRS IYKNKFELTY VGPSQLIVKN LSSGTRVVLK SHYGYEVEEV
KILGKERYLV AHTSDTLLLG DLNTNRLSEI PWQGSGGNEK YFFENENVCM IFNAGELTLV
EYGSNDSLGS VRTEFMNPHL ISVRINERCQ RGMEDNKKLA YLVDIKTIAI VDLIGGYNIG
TISHESRVDW LELNETGHKL LFRDRKLRLH LYDIESCSKT MILNFCSYVQ WVPGSDVLVA
QNRNSLCVWY NIEAPERVTM SSIRGDVVGL ERGGGKTEVM VTEGVTTVAY TLDEGLIEFG
TAIDDGNYTR ATAFLETLEM TPETEAMWKT LSKLALEARQ LHTAERCFSA LGHVAKARFL
HETNEIADQV SREYGGEGTD FYQVRARLAM LEKNYKLAEM IFLEQNAVEE AMDMYQELHR
WDECIAVAEA KGHPALEKLR RDYYQRLMDT QQEERAGELQ ESQGDGLAAI SLYLKAGLPA
KAARLVLTRE ELLANTELVE HITTALIKGE LYERAGDLFE KIRNPQRALE CYCKGNAFMK
AVELARLAFP VEVVRLEEAW GDHLVQQKQL DAAINHYIEA RCSIKAIEAA LGARQWKKAI
YILDLQDRNT ASKYYPRVAQ HYASLQEYEI AEELYTKGDR TKDAIDMYTQ AGRWEQAHKL
AMKCMRPEDV SVLYITQAQE MEKQGKYREA ERLYVTVEEP DLAITMFKKH KLYDDMIRLV
GKHHPDLLSD THLHLGKELE TEGRLQEAEY HYLEAQEWKA TVNMYRSSGL WEEAYRVAKA
HGGANAHKHV AYLWAKSLGG EAAVRLLNKL GLLEAAIDHA ADNCSFEFAF ELSRLALKHK
TPEIHLRYAM YLEDEGKFEE AEAEFIRAGK PKEAVLMFVH NQDWEAAQRV AEAHDPDSVA
EVLVGQARGA LEEKDFQKAE GLLLRAQRPG LALNYYKEAG LWSDALRICK DYVPGQLEAL
QEEYEREATK KGGRGVEGLV EQARQWEQAG EYSRAVDCYL KVRDSGSSGL MEKCWMKAAE
LSIKFLPPQR SLEVVRVVGP QLIGIGKHSA SAELYLNLDL VKEAIDAFIE GEEWNKAKRV
AKELDPRYED YVDQHYKEFL KNQGKVDSLV GVDVVAALDL YVEQGQWDKC IETATKQNYK
ILHKYVALYA THLIREGGYA QALALYVQHG APANPQNFNI YKRIFTDMVS SPGTNNAEAY
HSWADLRDVL FNLCENLVKS SEANSAAHEE FEMMLLIAHY YATRSAAQSI KQLETVAARL
SVSLLRHTQL LPADKAFYEA GTAAKEVGWE NMAFIFFNRF LDLTDAIEEG TLDALDHSDF
QDTDIPFEVP LPAKQHVPEA QREEVRDWVL TVSMDQRLEQ VLPRDERGVY EASLVAASTG
VRALPCLITG YPILRNKIEF KRPGKAANKD NWNKFLMAIK TSHSPVCQDV LKFISQWCGG
LPSTSFSFQ
//
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