ID GOT1B_HUMAN Reviewed; 138 AA.
AC Q9Y3E0; B2R4R4; Q54A40; Q6I9W6; Q9P1R9;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 02-NOV-2016, entry version 129.
DE RecName: Full=Vesicle transport protein GOT1B;
DE AltName: Full=Germ cell tumor 2;
DE AltName: Full=Golgi transport 1 homolog B;
DE AltName: Full=Putative NF-kappa-B-activating protein 470;
DE AltName: Full=hGOT1a;
GN Name=GOLT1B; Synonyms=GCT2, GOT1A;
GN ORFNames=CGI-141, HDCMA39P, UNQ432/PRO793;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Amygdala;
RX PubMed=11230166; DOI=10.1101/gr.GR1547R;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H.,
RA Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N.,
RA Mewes H.-W., Ottenwaelder B., Obermaier B., Tampe J., Heubner D.,
RA Wambutt R., Korn B., Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and
RT analysis of 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung fibroblast;
RX PubMed=12761501; DOI=10.1038/sj.onc.1206406;
RA Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O.,
RA Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H.,
RA Sugano S.;
RT "Large-scale identification and characterization of human genes that
RT activate NF-kappaB and MAPK signaling pathways.";
RL Oncogene 22:3307-3318(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Dendritic cell;
RA Zhao Z., Huang X., Li N., Zhu X., Cao X.;
RT "A novel gene from human dendritic cell.";
RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP PUTATIVE FUNCTION, SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=10406798; DOI=10.1093/emboj/18.14.3934;
RA Conchon S., Cao X., Barlowe C., Pelham H.R.;
RT "Got1p and Sft2p: membrane proteins involved in traffic to the Golgi
RT complex.";
RL EMBO J. 18:3934-3946(1999).
RN [11]
RP TISSUE SPECIFICITY.
RX PubMed=12414650;
RA Bourdon V., Naef F., Rao P.H., Reuter V., Mok S.C., Bosl G.J.,
RA Koul S., Murty V.V., Kucherlapati R.S., Chaganti R.S.;
RT "Genomic and expression analysis of the 12p11-p12 amplicon using EST
RT arrays identifies two novel amplified and overexpressed genes.";
RL Cancer Res. 62:6218-6223(2002).
RN [12]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.M111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C.,
RA Meinnel T., Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M.,
RA Ayoub D., Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: May be involved in fusion of ER-derived transport
CC vesicles with the Golgi complex.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:10406798}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:10406798}.
CC -!- TISSUE SPECIFICITY: Widely expressed. Tends to be up-regulated in
CC seminomas compared to normal testis.
CC {ECO:0000269|PubMed:12414650}.
CC -!- SIMILARITY: Belongs to the GOT1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF65181.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
DR EMBL; AF151899; AAD34136.1; -; mRNA.
DR EMBL; AL136571; CAB66506.1; -; mRNA.
DR EMBL; AB097020; BAC77373.1; -; mRNA.
DR EMBL; AY358975; AAQ89334.1; -; mRNA.
DR EMBL; AF068292; AAF65181.1; ALT_INIT; mRNA.
DR EMBL; CR457389; CAG33670.1; -; mRNA.
DR EMBL; AK311920; BAG34861.1; -; mRNA.
DR EMBL; CH471094; EAW96443.1; -; Genomic_DNA.
DR EMBL; BC012455; AAH12455.1; -; mRNA.
DR CCDS; CCDS8689.1; -.
DR PIR; T46908; T46908.
DR RefSeq; NP_057156.1; NM_016072.4.
DR UniGene; Hs.62275; -.
DR ProteinModelPortal; Q9Y3E0; -.
DR BioGrid; 119232; 176.
DR IntAct; Q9Y3E0; 177.
DR MINT; MINT-3085932; -.
DR STRING; 9606.ENSP00000229314; -.
DR iPTMnet; Q9Y3E0; -.
DR PhosphoSitePlus; Q9Y3E0; -.
DR BioMuta; GOLT1B; -.
DR DMDM; 20534680; -.
DR EPD; Q9Y3E0; -.
DR MaxQB; Q9Y3E0; -.
DR PaxDb; Q9Y3E0; -.
DR PeptideAtlas; Q9Y3E0; -.
DR PRIDE; Q9Y3E0; -.
DR TopDownProteomics; Q9Y3E0; -.
DR DNASU; 51026; -.
DR Ensembl; ENST00000229314; ENSP00000229314; ENSG00000111711.
DR GeneID; 51026; -.
DR KEGG; hsa:51026; -.
DR UCSC; uc001rez.3; human.
DR CTD; 51026; -.
DR DisGeNET; 51026; -.
DR GeneCards; GOLT1B; -.
DR HGNC; HGNC:20175; GOLT1B.
DR HPA; HPA066383; -.
DR MIM; 615078; gene.
DR neXtProt; NX_Q9Y3E0; -.
DR OpenTargets; ENSG00000111711; -.
DR PharmGKB; PA134898810; -.
DR eggNOG; KOG1743; Eukaryota.
DR eggNOG; COG5120; LUCA.
DR GeneTree; ENSGT00390000014507; -.
DR HOGENOM; HOG000035939; -.
DR HOVERGEN; HBG009362; -.
DR InParanoid; Q9Y3E0; -.
DR OMA; AGESNNM; -.
DR OrthoDB; EOG091G10MS; -.
DR PhylomeDB; Q9Y3E0; -.
DR TreeFam; TF300267; -.
DR GeneWiki; GOLT1B; -.
DR GenomeRNAi; 51026; -.
DR PRO; PR:Q9Y3E0; -.
DR Proteomes; UP000005640; Chromosome 12.
DR Bgee; ENSG00000111711; -.
DR CleanEx; HS_GOLT1B; -.
DR ExpressionAtlas; Q9Y3E0; baseline and differential.
DR Genevisible; Q9Y3E0; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:LIFEdb.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR GO; GO:0004871; F:signal transducer activity; IMP:UniProtKB.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR InterPro; IPR007305; Vesicle_transpt_Got1/SFT2.
DR Pfam; PF04178; Got1; 1.
PE 1: Evidence at protein level;
KW Acetylation; Complete proteome; Golgi apparatus; Membrane;
KW Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1 138 Vesicle transport protein GOT1B.
FT /FTId=PRO_0000218582.
FT TOPO_DOM 1 9 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 10 30 Helical; Name=1. {ECO:0000255}.
FT TOPO_DOM 31 32 Lumenal. {ECO:0000255}.
FT TRANSMEM 33 53 Helical; Name=2. {ECO:0000255}.
FT TOPO_DOM 54 68 Cytoplasmic. {ECO:0000255}.
FT TRANSMEM 69 89 Helical; Name=3. {ECO:0000255}.
FT TOPO_DOM 90 90 Lumenal. {ECO:0000255}.
FT TRANSMEM 91 109 Helical; Name=4. {ECO:0000255}.
FT TOPO_DOM 110 138 Cytoplasmic. {ECO:0000255}.
FT COMPBIAS 17 108 Phe-rich.
FT MOD_RES 1 1 N-acetylmethionine.
FT {ECO:0000244|PubMed:22223895}.
FT CONFLICT 37 37 I -> T (in Ref. 5; AAF65181).
FT {ECO:0000305}.
FT CONFLICT 54 54 R -> K (in Ref. 6; CAG33670).
FT {ECO:0000305}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 17 113 ipfam:Got1 [T]
SQ SEQUENCE 138 AA; 15426 MW; 2EB85823C34EFAFA CRC64;
MISLTDTQKI GMGLTGFGVF FLFFGMILFF DKALLAIGNV LFVAGLAFVI GLERTFRFFF
QKHKMKATGF FLGGVFVVLI GWPLIGMIFE IYGFFLLFRG FFPVVVGFIR RVPVLGSLLN
LPGIRSFVDK VGESNNMV
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