Entry sw:FOLD_BURCH

ID   FOLD_BURCH              Reviewed;         285 AA.
AC   A0K8R4;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   02-NOV-2016, entry version 70.
DE   RecName: Full=Bifunctional protein FolD {ECO:0000255|HAMAP-Rule:MF_01576};
DE   Includes:
DE     RecName: Full=Methylenetetrahydrofolate dehydrogenase {ECO:0000255|HAMAP-Rule:MF_01576};
DE              EC=1.5.1.5 {ECO:0000255|HAMAP-Rule:MF_01576};
DE   Includes:
DE     RecName: Full=Methenyltetrahydrofolate cyclohydrolase {ECO:0000255|HAMAP-Rule:MF_01576};
DE              EC=3.5.4.9 {ECO:0000255|HAMAP-Rule:MF_01576};
GN   Name=folD {ECO:0000255|HAMAP-Rule:MF_01576};
GN   OrderedLocusNames=Bcen2424_2140;
OS   Burkholderia cenocepacia (strain HI2424).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=331272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HI2424;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., LiPuma J.J., Gonzalez C.F.,
RA   Konstantinidis K., Tiedje J.M., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cenocepacia
RT   HI2424.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the oxidation of 5,10-
CC       methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and
CC       then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-
CC       formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_01576}.
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + NADP(+) =
CC       5,10-methenyltetrahydrofolate + NADPH. {ECO:0000255|HAMAP-
CC       Rule:MF_01576}.
CC   -!- CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H(2)O = 10-
CC       formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_01576}.
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01576}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01576}.
CC   -!- SIMILARITY: Belongs to the tetrahydrofolate
CC       dehydrogenase/cyclohydrolase family. {ECO:0000255|HAMAP-
CC       Rule:MF_01576}.
DR   EMBL; CP000458; ABK08891.1; -; Genomic_DNA.
DR   RefSeq; WP_011694326.1; NC_008542.1.
DR   ProteinModelPortal; A0K8R4; -.
DR   EnsemblBacteria; ABK08891; ABK08891; Bcen2424_2140.
DR   KEGG; bch:Bcen2424_2140; -.
DR   PATRIC; 19061653; VBIBurCen15205_2178.
DR   HOGENOM; HOG000218242; -.
DR   KO; K01491; -.
DR   OMA; RVHDPIT; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
DR   GO; GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.720; -; 1.
DR   HAMAP; MF_01576; THF_DHG_CYH; 1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR000672; THF_DH/CycHdrlase.
DR   InterPro; IPR020630; THF_DH/CycHdrlase_cat_dom.
DR   InterPro; IPR020867; THF_DH/CycHdrlase_CS.
DR   InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom.
DR   Pfam; PF00763; THF_DHG_CYH; 1.
DR   Pfam; PF02882; THF_DHG_CYH_C; 1.
DR   PRINTS; PR00085; THFDHDRGNASE.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00766; THF_DHG_CYH_1; 1.
DR   PROSITE; PS00767; THF_DHG_CYH_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Histidine biosynthesis; Hydrolase;
KW   Methionine biosynthesis; Multifunctional enzyme; NADP;
KW   One-carbon metabolism; Oxidoreductase; Purine biosynthesis.
FT   CHAIN         1    285       Bifunctional protein FolD.
FT                                /FTId=PRO_0000305802.
FT   NP_BIND     165    167       NADP. {ECO:0000255|HAMAP-Rule:MF_01576}.
FT   BINDING     190    190       NADP. {ECO:0000255|HAMAP-Rule:MF_01576}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT         5    120       ipfam:THF_DHG_CYH [T]
FT   MYHIT        75    100       ipat:THF_DHG_CYH_1 [T]
FT   MYHIT       259    267       ipat:THF_DHG_CYH_2 [T]
FT   MYHIT       123    281       ipfam:THF_DHG_CYH_C [T]
FT   MYHIT         3    282       ihamap:THF_DHG_CYH [T]
SQ   SEQUENCE   285 AA;  29787 MW;  71EA67C7DBE813D7 CRC64;
     MTALLIDGNA LSKTLRAQAA ERAAALTARG HQPGLAVILV GANPASEVYV RNKIKACEDN
     GFFSLKDAYP ATLSEADLLA RIDELNRDPK IHGILVQLPL PAHIDSHKVI EAIAPEKDVD
     GFHVANAGAL MTGKPLFRPC TPYGVMKMFE AHGIALQGAN AVVIGRSNIV GKPMAMMLLD
     AGATVTICHS KTRDLAAHTR EADIVVAAVG KRNILTADMV KPGATVIDVG MNRDDAGKLC
     GDVDFAGVKE VAGYITPVPG GVGPMTITML LINTIESAER AAAAA
//