ID FDNI_SHIFL Reviewed; 217 AA.
AC P0AEK9; P24185; P77513;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 02-NOV-2016, entry version 82.
DE RecName: Full=Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunit;
DE AltName: Full=Anaerobic formate dehydrogenase cytochrome b556 subunit;
DE AltName: Full=Formate dehydrogenase-N subunit gamma;
DE Short=FDH-N subunit gamma;
GN Name=fdnI; OrderedLocusNames=SF1749, S1882;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H.,
RA Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J.,
RA Sun L., Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S.,
RA Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y.,
RA Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/IAI.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W.,
RA Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A.,
RA Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S.,
RA Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella
RT flexneri serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Formate dehydrogenase allows the bacterium to use
CC formate as major electron donor during anaerobic respiration, when
CC nitrate is used as electron acceptor. Subunit gamma is the
CC cytochrome b556 component of the formate dehydrogenase-N, and also
CC contains a menaquinone reduction site that receives electrons from
CC the beta subunit (FdnH), through its hemes. Formate dehydrogenase-
CC N is part of a system that generates proton motive force, together
CC with the dissimilatory nitrate reductase (Nar) (By similarity).
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC Note=Binds 2 heme groups per subunit. Heme 1 is located at the
CC cytoplasmic interface, heme 2 is located at the periplasmic
CC interface. Electrons are transferred from the periplasmic to the
CC cytoplasmic heme. {ECO:0000250};
CC -!- SUBUNIT: Trimer of heterotrimers, consisting of subunits alpha,
CC beta and gamma. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the formate dehydrogenase gamma subunit
CC family. {ECO:0000305}.
DR EMBL; AE005674; AAN43321.2; -; Genomic_DNA.
DR EMBL; AE014073; AAP17207.1; -; Genomic_DNA.
DR RefSeq; NP_707614.2; NC_004337.2.
DR RefSeq; WP_000045648.1; NZ_LVJC01000105.1.
DR ProteinModelPortal; P0AEK9; -.
DR SMR; P0AEK9; -.
DR STRING; 198214.SF1749; -.
DR PaxDb; P0AEK9; -.
DR PRIDE; P0AEK9; -.
DR EnsemblBacteria; AAN43321; AAN43321; SF1749.
DR EnsemblBacteria; AAP17207; AAP17207; S1882.
DR GeneID; 1024930; -.
DR KEGG; sfl:SF1749; -.
DR KEGG; sft:NCTC1_01894; -.
DR KEGG; sfx:S1882; -.
DR PATRIC; 18705181; VBIShiFle31049_2086.
DR eggNOG; ENOG4108MG3; Bacteria.
DR eggNOG; COG2864; LUCA.
DR HOGENOM; HOG000163500; -.
DR KO; K08350; -.
DR OMA; KQDIPWL; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR GO; GO:0008863; F:formate dehydrogenase (NAD+) activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0022904; P:respiratory electron transport chain; IEA:InterPro.
DR InterPro; IPR011577; Cyt_b561_bac/Ni-Hgenase.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR InterPro; IPR006471; Formate_DH_gsu.
DR Pfam; PF01292; Ni_hydr_CYTB; 1.
DR SUPFAM; SSF81342; SSF81342; 1.
DR TIGRFAMs; TIGR01583; formate-DH-gamm; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Complete proteome;
KW Electron transport; Heme; Iron; Membrane; Metal-binding;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 217 Formate dehydrogenase, nitrate-inducible,
FT cytochrome b556(Fdn) subunit.
FT /FTId=PRO_0000087212.
FT TOPO_DOM 1 11 Cytoplasmic. {ECO:0000250}.
FT TRANSMEM 12 36 Helical. {ECO:0000250}.
FT TOPO_DOM 37 52 Periplasmic. {ECO:0000250}.
FT TRANSMEM 53 74 Helical. {ECO:0000250}.
FT TOPO_DOM 75 110 Cytoplasmic. {ECO:0000250}.
FT TRANSMEM 111 134 Helical. {ECO:0000250}.
FT TOPO_DOM 135 150 Periplasmic. {ECO:0000250}.
FT TRANSMEM 151 175 Helical. {ECO:0000250}.
FT TOPO_DOM 176 217 Cytoplasmic. {ECO:0000250}.
FT METAL 18 18 Iron (heme B 1 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT METAL 57 57 Iron (heme B 2 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT METAL 155 155 Iron (heme B 2 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT METAL 169 169 Iron (heme B 1 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT BINDING 169 169 Menaquinone; via pros nitrogen.
FT {ECO:0000250}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 13 186 ipfam:Ni_hydr_CYTB [T]
SQ SEQUENCE 217 AA; 25368 MW; B10073F14343515E CRC64;
MSKSKMIVRT KFIDRACHWT VVICFFLVAL SGISFFFPTL QWLTQTFGTP QMGRILHPFF
GIAIFVALMF MFVRFVHHNI PDKKDIPWLL NIVEVLKGNE HKVADVGKYN AGQKMMFWSI
MSMIFVLLVT GVIIWRPYFA QYFPMQVVRY SLLIHAAAGI ILIHAILIHM YMAFWVKGSI
KGMIEGKVSR RWAKKHHPRW YREIEKAEAK KESEEGI
//
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