ID FDNI_ECO57 Reviewed; 217 AA.
AC P0AEK8; P24185; P77513;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 02-NOV-2016, entry version 85.
DE RecName: Full=Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunit;
DE AltName: Full=Anaerobic formate dehydrogenase cytochrome b556 subunit;
DE AltName: Full=Formate dehydrogenase-N subunit gamma;
DE Short=FDH-N subunit gamma;
GN Name=fdnI; OrderedLocusNames=Z2234, ECs2080;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA Welch R.A., Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli
RT O157:H7 and genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Formate dehydrogenase allows the bacterium to use
CC formate as major electron donor during anaerobic respiration, when
CC nitrate is used as electron acceptor. Subunit gamma is the
CC cytochrome b556 component of the formate dehydrogenase-N, and also
CC contains a menaquinone reduction site that receives electrons from
CC the beta subunit (FdnH), through its hemes. Formate dehydrogenase-
CC N is part of a system that generates proton motive force, together
CC with the dissimilatory nitrate reductase (Nar) (By similarity).
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC Note=Binds 2 heme groups per subunit. Heme 1 is located at the
CC cytoplasmic interface, heme 2 is located at the periplasmic
CC interface. Electrons are transferred from the periplasmic to the
CC cytoplasmic heme. {ECO:0000250};
CC -!- SUBUNIT: Trimer of heterotrimers, consisting of subunits alpha,
CC beta and gamma. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the formate dehydrogenase gamma subunit
CC family. {ECO:0000305}.
DR EMBL; AE005174; AAG56293.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB35503.1; -; Genomic_DNA.
DR PIR; A85729; A85729.
DR PIR; H90888; H90888.
DR RefSeq; NP_310107.1; NC_002695.1.
DR RefSeq; WP_000045648.1; NZ_LPWC02000002.1.
DR ProteinModelPortal; P0AEK8; -.
DR SMR; P0AEK8; -.
DR STRING; 155864.Z2234; -.
DR EnsemblBacteria; AAG56293; AAG56293; Z2234.
DR EnsemblBacteria; BAB35503; BAB35503; BAB35503.
DR GeneID; 917301; -.
DR KEGG; ece:Z2234; -.
DR KEGG; ecs:ECs2080; -.
DR PATRIC; 18353185; VBIEscCol44059_1812.
DR eggNOG; ENOG4108MG3; Bacteria.
DR eggNOG; COG2864; LUCA.
DR HOGENOM; HOG000163500; -.
DR KO; K08350; -.
DR OMA; KQDIPWL; -.
DR BioCyc; ECOO157:FDNI-MONOMER; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR GO; GO:0008863; F:formate dehydrogenase (NAD+) activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0022904; P:respiratory electron transport chain; IEA:InterPro.
DR InterPro; IPR011577; Cyt_b561_bac/Ni-Hgenase.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR InterPro; IPR006471; Formate_DH_gsu.
DR Pfam; PF01292; Ni_hydr_CYTB; 1.
DR SUPFAM; SSF81342; SSF81342; 1.
DR TIGRFAMs; TIGR01583; formate-DH-gamm; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Complete proteome;
KW Electron transport; Heme; Iron; Membrane; Metal-binding;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 217 Formate dehydrogenase, nitrate-inducible,
FT cytochrome b556(Fdn) subunit.
FT /FTId=PRO_0000087211.
FT TOPO_DOM 1 11 Cytoplasmic. {ECO:0000250}.
FT TRANSMEM 12 36 Helical. {ECO:0000250}.
FT TOPO_DOM 37 52 Periplasmic. {ECO:0000250}.
FT TRANSMEM 53 74 Helical. {ECO:0000250}.
FT TOPO_DOM 75 110 Cytoplasmic. {ECO:0000250}.
FT TRANSMEM 111 134 Helical. {ECO:0000250}.
FT TOPO_DOM 135 150 Periplasmic. {ECO:0000250}.
FT TRANSMEM 151 175 Helical. {ECO:0000250}.
FT TOPO_DOM 176 217 Cytoplasmic. {ECO:0000250}.
FT METAL 18 18 Iron (heme B 1 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT METAL 57 57 Iron (heme B 2 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT METAL 155 155 Iron (heme B 2 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT METAL 169 169 Iron (heme B 1 axial ligand); via tele
FT nitrogen. {ECO:0000250}.
FT BINDING 169 169 Menaquinone; via pros nitrogen.
FT {ECO:0000250}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 13 186 ipfam:Ni_hydr_CYTB [T]
SQ SEQUENCE 217 AA; 25368 MW; B10073F14343515E CRC64;
MSKSKMIVRT KFIDRACHWT VVICFFLVAL SGISFFFPTL QWLTQTFGTP QMGRILHPFF
GIAIFVALMF MFVRFVHHNI PDKKDIPWLL NIVEVLKGNE HKVADVGKYN AGQKMMFWSI
MSMIFVLLVT GVIIWRPYFA QYFPMQVVRY SLLIHAAAGI ILIHAILIHM YMAFWVKGSI
KGMIEGKVSR RWAKKHHPRW YREIEKAEAK KESEEGI
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