ID DUT_RHOCS Reviewed; 151 AA.
AC B6IVT5;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 02-NOV-2016, entry version 49.
DE RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase {ECO:0000255|HAMAP-Rule:MF_00116};
DE Short=dUTPase {ECO:0000255|HAMAP-Rule:MF_00116};
DE EC=3.6.1.23 {ECO:0000255|HAMAP-Rule:MF_00116};
DE AltName: Full=dUTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_00116};
GN Name=dut {ECO:0000255|HAMAP-Rule:MF_00116};
GN OrderedLocusNames=RC1_3043;
OS Rhodospirillum centenum (strain ATCC 51521 / SW).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Rhodospirillum.
OX NCBI_TaxID=414684;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51521 / SW;
RA Touchman J.W., Bauer C., Blankenship R.E.;
RT "Genome sequence of Rhodospirillum centenum.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This enzyme is involved in nucleotide metabolism: it
CC produces dUMP, the immediate precursor of thymidine nucleotides
CC and it decreases the intracellular concentration of dUTP so that
CC uracil cannot be incorporated into DNA. {ECO:0000255|HAMAP-
CC Rule:MF_00116}.
CC -!- CATALYTIC ACTIVITY: dUTP + H(2)O = dUMP + diphosphate.
CC {ECO:0000255|HAMAP-Rule:MF_00116}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00116};
CC -!- PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP
CC (dUTP route): step 2/2. {ECO:0000255|HAMAP-Rule:MF_00116}.
CC -!- SIMILARITY: Belongs to the dUTPase family. {ECO:0000255|HAMAP-
CC Rule:MF_00116}.
DR EMBL; CP000613; ACJ00409.1; -; Genomic_DNA.
DR RefSeq; WP_012568189.1; NC_011420.2.
DR ProteinModelPortal; B6IVT5; -.
DR STRING; 414684.RC1_3043; -.
DR EnsemblBacteria; ACJ00409; ACJ00409; RC1_3043.
DR KEGG; rce:RC1_3043; -.
DR PATRIC; 23321260; VBIRhoCen1465_2951.
DR eggNOG; ENOG4108Z1K; Bacteria.
DR eggNOG; COG0756; LUCA.
DR HOGENOM; HOG000028968; -.
DR KO; K01520; -.
DR OMA; IACPNAP; -.
DR OrthoDB; POG091H01I6; -.
DR UniPathway; UPA00610; UER00666.
DR Proteomes; UP000001591; Chromosome.
DR GO; GO:0004170; F:dUTP diphosphatase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0006226; P:dUMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0046080; P:dUTP metabolic process; IEA:InterPro.
DR CDD; cd07557; trimeric_dUTPase; 1.
DR Gene3D; 2.70.40.10; -; 1.
DR HAMAP; MF_00116; dUTPase_bact; 1.
DR InterPro; IPR029054; dUTPase-like.
DR InterPro; IPR008180; dUTPase/dCTP_deaminase.
DR InterPro; IPR008181; dUTPase_1.
DR InterPro; IPR033704; dUTPase_trimeric.
DR Pfam; PF00692; dUTPase; 1.
DR SUPFAM; SSF51283; SSF51283; 1.
DR TIGRFAMs; TIGR00576; dut; 1.
PE 3: Inferred from homology;
KW Complete proteome; Hydrolase; Magnesium; Metal-binding;
KW Nucleotide metabolism; Reference proteome.
FT CHAIN 1 151 Deoxyuridine 5'-triphosphate
FT nucleotidohydrolase.
FT /FTId=PRO_1000094984.
FT REGION 69 71 Substrate binding. {ECO:0000255|HAMAP-
FT Rule:MF_00116}.
FT REGION 86 88 Substrate binding. {ECO:0000255|HAMAP-
FT Rule:MF_00116}.
FT BINDING 82 82 Substrate. {ECO:0000255|HAMAP-
FT Rule:MF_00116}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 18 148 ipfam:dUTPase [T]
FT MYHIT 4 149 ihamap:dUTPase_bact [T]
SQ SEQUENCE 151 AA; 15639 MW; 721C98F1BB986727 CRC64;
MTDLSIAVTR LPHGADLPLP AYATEHAAGM DLLAAVAEPV ILAPGERRLI PTGLAIALPD
GYEAQVRPRS GLALKHGITL LNSPGTIDAD YRGEVGVILA NLGADPFTVE RGMRIAQMVI
ARYARAAWDV VDSLPASARG SGGFGSTGTR G
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