Legends: 1, CONFLICT G -> R (in Ref. 1; AAG43170). {ECO:0000305}; 2, CONFLICT M -> T (in Ref. 1; AAG43170). {ECO:0000305}; 3, CONFLICT L -> I (in Ref. 1; AAG43170). {ECO:0000305}; 4, ipfam:DNA_pol_A_exo1 [T]; 5, ipat:DNA_POLYMERASE_A [T]; 6, ipfam:5_3_exonuc_N [T]; 7, ismart:HhH2 [T]; 8, ipfam:5_3_exonuc [T].
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ID DPO1_SALTY Reviewed; 928 AA.
AC Q9F173;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 18-JAN-2017, entry version 114.
DE RecName: Full=DNA polymerase I;
DE Short=POL I;
DE EC=2.7.7.7;
GN Name=polA; OrderedLocusNames=STM3999;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RA Huang Y.P., Ito J.;
RT "DNA polymerase I sequence from Salmonella typhimurium.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA Waterston R., Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 3'-5' and 5'-3' exonuclease activity. It is able to
CC utilize nicked circular duplex DNA as a template and can unwind
CC the parental DNA strand from its template.
CC -!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
CC diphosphate + DNA(n+1).
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 3'-5' exonuclease domain. {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 5'-3' exonuclease domain. {ECO:0000305}.
DR EMBL; AF071212; AAG43170.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22838.1; -; Genomic_DNA.
DR RefSeq; NP_462879.1; NC_003197.1.
DR RefSeq; WP_000249972.1; NC_003197.1.
DR ProteinModelPortal; Q9F173; -.
DR STRING; 99287.STM3999; -.
DR PaxDb; Q9F173; -.
DR PRIDE; Q9F173; -.
DR EnsemblBacteria; AAL22838; AAL22838; STM3999.
DR GeneID; 1255525; -.
DR KEGG; stm:STM3999; -.
DR PATRIC; 32386868; VBISalEnt20916_4214.
DR eggNOG; ENOG4105C2M; Bacteria.
DR eggNOG; COG0258; LUCA.
DR eggNOG; COG0749; LUCA.
DR HOGENOM; HOG000020999; -.
DR KO; K02335; -.
DR OMA; CFDTETT; -.
DR PhylomeDB; Q9F173; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-dependent DNA replication; IBA:GO_Central.
DR Gene3D; 3.30.420.10; -; 1.
DR Gene3D; 3.40.50.1010; -; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR020045; 5-3_exonuclease_C.
DR InterPro; IPR002421; 5-3_exonuclease_N.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN_domain-like.
DR InterPro; IPR012337; RNaseH-like_dom.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR TIGRFAMs; TIGR00593; pola; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW Complete proteome; DNA damage; DNA repair; DNA replication;
KW DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase;
KW Nuclease; Nucleotidyltransferase; Reference proteome; Transferase.
FT CHAIN 1 928 DNA polymerase I.
FT /FTId=PRO_0000101252.
FT DOMAIN 1 323 5'-3' exonuclease.
FT DOMAIN 324 517 3'-5' exonuclease.
FT REGION 324 928 Klenow fragment.
FT REGION 521 928 Polymerase.
FT CONFLICT 751 751 G -> R (in Ref. 1; AAG43170).
FT {ECO:0000305}.
FT CONFLICT 848 848 M -> T (in Ref. 1; AAG43170).
FT {ECO:0000305}.
FT CONFLICT 867 867 L -> I (in Ref. 1; AAG43170).
FT {ECO:0000305}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 330 517 ipfam:DNA_pol_A_exo1 [T]
FT MYHIT 7 268 ismart:53EXOc [T]
FT MYHIT 552 925 ipfam:DNA_pol_A [T]
FT MYHIT 686 892 ismart:POLAc [T]
FT MYHIT 754 773 ipat:DNA_POLYMERASE_A [T]
FT MYHIT 9 170 ipfam:5_3_exonuc_N [T]
FT MYHIT 173 208 ismart:HhH2 [T]
FT MYHIT 171 270 ipfam:5_3_exonuc [T]
FT MYHIT 330 517 ismart:35EXOc [T]
SQ SEQUENCE 928 AA; 103129 MW; FF887BF1039C160A CRC64;
MVQIPENPLI LVDGSSYLYR AYHAFPPLTN SAGEPTGAMY GVLNMLRSLI MQYQPTHAAV
VFDAKGKTFR DELFEHYKSH RPPMPDDLRA QIEPLHAMVK AMGLPLLAVS GVEADDVIGT
LAREAEKVGR PVLISTGDKD MAQLVTPNIT LINTMTNTIL GPDEVVNKYG VPPELIIDFL
ALMGDSSDNI PGVPGVGEKT AQALLQGLGG LDTLYAEPEK IAGLTFRGAK TMAGKLAQNK
DVAYLSYKLA TIKTDVELEL TCEQLEVQQP IADELLGLFK KYEFKRWTAD VESGKWLQAK
GAKPAAKPQE TVVIDESPSE PAAALSYENY VTILDDVTLE SWIEKLKKAP VFAFDTETDS
LDNIAANLVG LSFAIEPGVA AYVPVAHDYL DAPDQISRQR ALELLKPLLE DEKVRKVGQN
LKYDRGVLQN YGIELRGIAF DTMLESYILN SVAGRHDMDS LSDRWLKHKT ITFEDIAGKG
KNQLTFNQIA LEEAGRYAAE DADVTLQLHL KMWPELQQHK GPLNVFENIE MPLVPVLSRV
ERNGVKIDPA VLHKHSEEIT LRLAELEKKA HDIAGEAFNL SSTKQLQTIL FEKQGIKPLK
KTPGGAPSTS EEVLEELALD YPLPKVILEY RGLAKLKSTY TDKLPLMINP KTGRVHTSYH
QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYL IVSADYSQIE LRIMAHLSRD
KGLLTAFAEG KDIHRATAAE VFGLPLDSVT GEQRRSAKAI NFGLIYGMSA FGLSRQLNIP
RKEAQKYMDL YFERYPGVLE YMERTRAQAK EQGYVETLEG RRLYLPDIKS SNAARRAGAE
RAAINAPMQG TAADIIKRAM IAVDAWLQAE QPRVRMIMQV HDELVFEVHK DDLDAVAKRI
HQLMENCTRI DVPLLVEVGS GENWDQAH
//
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