ID CR1FA_BACTA Reviewed; 1174 AA.
AC Q03746;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 05-OCT-2016, entry version 67.
DE RecName: Full=Pesticidal crystal protein Cry1Fa;
DE AltName: Full=134 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIF(a);
GN Name=cry1Fa; Synonyms=cryIF, cryIF(a);
OS Bacillus thuringiensis subsp. aizawai.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1433;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=EG6346;
RX PubMed=2061280;
RA Chambers J.A., Jelen A., Gilbert M.P., Jany C.S., Johnson T.B.,
RA Gawron-Burke C.;
RT "Isolation and characterization of a novel insecticidal crystal
RT protein gene from Bacillus thuringiensis subsp. aizawai.";
RL J. Bacteriol. 173:3966-3976(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NRRL B-18484 / PS81I;
RA Payne J.M., Sick A.J.;
RT "Bacillus thuringiensis isolate active against lepidopteran pests, and
RT genes encoding novel lepidopteran-active toxins.";
RL Patent number US5188960, 23-FEB-1993.
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of many lepidopteran larvae.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during
CC sporulation and is accumulated both as an inclusion and as part of
CC the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
DR EMBL; M63897; AAA22348.1; -; Genomic_DNA.
DR EMBL; M73254; AAA22347.1; -; Genomic_DNA.
DR PIR; A42459; A42459.
DR ProteinModelPortal; Q03746; -.
DR PRIDE; Q03746; -.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR GO; GO:0009405; P:pathogenesis; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR Gene3D; 2.60.120.260; -; 1.
DR InterPro; IPR005638; Endotoxin_C.
DR InterPro; IPR001178; Endotoxin_cen_dom.
DR InterPro; IPR015790; Endotoxin_cen_dom_subgr1.
DR InterPro; IPR005639; Endotoxin_N.
DR InterPro; IPR008979; Galactose-bd-like.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 2: Evidence at transcript level;
KW Sporulation; Toxin.
FT CHAIN 1 1174 Pesticidal crystal protein Cry1Fa.
FT /FTId=PRO_0000174042.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 257 454 ipfam:Endotoxin_M [T]
FT MYHIT 464 601 ipfam:Endotoxin_C [T]
FT MYHIT 48 249 ipfam:Endotoxin_N [T]
SQ SEQUENCE 1174 AA; 133622 MW; B51E9751D7F91C61 CRC64;
MENNIQNQCV PYNCLNNPEV EILNEERSTG RLPLDISLSL TRFLLSEFVP GVGVAFGLFD
LIWGFITPSD WSLFLLQIEQ LIEQRIETLE RNRAITTLRG LADSYEIYIE ALREWEANPN
NAQLREDVRI RFANTDDALI TAINNFTLTS FEIPLLSVYV QAANLHLSLL RDAVSFGQGW
GLDIATVNNH YNRLINLIHR YTKHCLDTYN QGLENLRGTN TRQWARFNQF RRDLTLTVLD
IVALFPNYDV RTYPIQTSSQ LTREIYTSSV IEDSPVSANI PNGFNRAEFG VRPPHLMDFM
NSLFVTAETV RSQTVWGGHL VSSRNTAGNR INFPSYGVFN PGGAIWIADE DPRPFYRTLS
DPVFVRGGFG NPHYVLGLRG VAFQQTGTNH TRTFRNSGTI DSLDEIPPQD NSGAPWNDYS
HVLNHVTFVR WPGEISGSDS WRAPMFSWTH RSATPTNTID PERITQIPLV KAHTLQSGTT
VVRGPGFTGG DILRRTSGGP FAYTIVNING QLPQRYRARI RYASTTNLRI YVTVAGERIF
AGQFNKTMDT GDPLTFQSFS YATINTAFTF PMSQSSFTVG ADTFSSGNEV YIDRFELIPV
TATFEAEYDL ERAQKAVNAL FTSINQIGIK TDVTDYHIDQ VSNLVDCLSD EFCLDEKREL
SEKVKHAKRL SDERNLLQDP NFKGINRQLD RGWRGSTDIT IQRGDDVFKE NYVTLPGTFD
ECYPTYLYQK IDESKLKPYT RYQLRGYIED SQDLEIYLIR YNAKHETVNV LGTGSLWPLS
VQSPIRKCGE PNRCAPHLEW NPDLDCSCRD GEKCAHHSHH FSLDIDVGCT DLNEDLDVWV
IFKIKTQDGH ARLGNLEFLE EKPLVGEALA RVKRAEKKWR DKREKLELET NIVYKEAKES
VDALFVNSQY DQLQADTNIA MIHAADKRVH RIREAYLPEL SVIPGVNVDI FEELKGRIFT
AFFLYDARNV IKNGDFNNGL SCWNVKGHVD VEEQNNHRSV LVVPEWEAEV SQEVRVCPGR
GYILRVTAYK EGYGEGCVTI HEIENNTDEL KFSNCVEEEV YPNNTVTCND YTANQEEYGG
AYTSRNRGYD ETYGSNSSVP ADYASVYEEK SYTDGRRDNP CESNRGYGDY TPLPAGYVTK
ELEYFPETDK VWIEIGETEG TFIVDSVELL LMEE
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