Entry sw:CPNE2_HUMAN

ID   CPNE2_HUMAN             Reviewed;         548 AA.
AC   Q96FN4; Q68D19; Q719H8; Q86XP9;
DT   28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   15-AUG-2003, sequence version 3.
DT   30-NOV-2016, entry version 123.
DE   RecName: Full=Copine-2 {ECO:0000305};
DE   AltName: Full=Copine II {ECO:0000250|UniProtKB:Q99829, ECO:0000312|HGNC:HGNC:2315};
GN   Name=CPNE2 {ECO:0000312|HGNC:HGNC:2315};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Placenta;
RA   Ding P., Han W., Shi S., Rui M., Wang Y., Song Q., Ma D.;
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Zhang D.L., Cai J.J., Ma D.L.;
RT   "Cloning and characterization of a novel human gene encoding a protein
RT   of 446 amino acids.";
RL   Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15616553; DOI=10.1038/nature03187;
RA   Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X.,
RA   Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A.,
RA   Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.,
RA   Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L.,
RA   Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A.,
RA   Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D.,
RA   Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J.,
RA   Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA   Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I.,
RA   Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W.,
RA   Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A.,
RA   Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S.,
RA   Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA   Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA   Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L.,
RA   Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A.,
RA   Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L.,
RA   Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N.,
RA   Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M.,
RA   Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L.,
RA   Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D.,
RA   Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P.,
RA   Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M.,
RA   Rubin E.M., Pennacchio L.A.;
RT   "The sequence and analysis of duplication-rich human chromosome 16.";
RL   Nature 432:988-994(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Pancreas;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-548 (ISOFORM 1).
RC   TISSUE=Fetal brain;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA   Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA   Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [6]
RP   TISSUE SPECIFICITY.
RX   PubMed=12949241; DOI=10.1189/jlb.0203083;
RA   Cowland J.B., Carter D., Bjerregaard M.D., Johnsen A.H.,
RA   Borregaard N., Lollike K.;
RT   "Tissue expression of copines and isolation of copines I and III from
RT   the cytosol of human neutrophils.";
RL   J. Leukoc. Biol. 74:379-388(2003).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
CC   -!- FUNCTION: Calcium-dependent phospholipid-binding protein that
CC       plays a role in calcium-mediated intracellular processes. Exhibits
CC       calcium-dependent cell membrane binding properties.
CC       {ECO:0000250|UniProtKB:P59108}.
CC   -!- INTERACTION:
CC       Q8TBB1:LNX1; NbExp=3; IntAct=EBI-7097057, EBI-739832;
CC       Q04864:REL; NbExp=3; IntAct=EBI-7097057, EBI-307352;
CC       O60504:SORBS3; NbExp=5; IntAct=EBI-7097057, EBI-741237;
CC       Q9NYB0:TERF2IP; NbExp=2; IntAct=EBI-7097057, EBI-750109;
CC       P14373:TRIM27; NbExp=5; IntAct=EBI-7097057, EBI-719493;
CC       Q8TBC4:UBA3; NbExp=5; IntAct=EBI-7097057, EBI-717567;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P59108}.
CC       Nucleus {ECO:0000250|UniProtKB:P59108}. Cell membrane
CC       {ECO:0000250|UniProtKB:P59108}. Note=Translocates to the cell
CC       membrane and the nucleus in a calcium-dependent manner.
CC       Colocalizes with CD2 at the cell membrane.
CC       {ECO:0000250|UniProtKB:P59108}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q96FN4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96FN4-2; Sequence=VSP_055536;
CC   -!- TISSUE SPECIFICITY: Expressed in the brain. Expressed in
CC       neutrophil precursors from the bone marrow and peripheral blood
CC       (PubMed:12949241).
CC   -!- DOMAIN: The C2 domain 2, but not the C2 domain 1, is necessary for
CC       calcium-mediated membrane association. The linker region is
CC       necessary for calcium-dependent cell membrane association.
CC       {ECO:0000250|UniProtKB:P59108}.
CC   -!- SIMILARITY: Belongs to the copine family. {ECO:0000305}.
CC   -!- SIMILARITY: Contains 2 C2 domains. {ECO:0000255|PROSITE-
CC       ProRule:PRU00041}.
CC   -!- SIMILARITY: Contains 1 VWFA domain. {ECO:0000255|PROSITE-
CC       ProRule:PRU00219}.
DR   EMBL; AF492484; AAO85483.1; -; mRNA.
DR   EMBL; AF542551; AAQ09534.1; -; mRNA.
DR   EMBL; AC009090; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC053658; AAH53658.1; -; mRNA.
DR   EMBL; CR749617; CAH18411.1; -; mRNA.
DR   CCDS; CCDS10774.1; -. [Q96FN4-1]
DR   RefSeq; NP_689940.3; NM_152727.5. [Q96FN4-1]
DR   UniGene; Hs.339809; -.
DR   UniGene; Hs.736510; -.
DR   ProteinModelPortal; Q96FN4; -.
DR   SMR; Q96FN4; -.
DR   BioGrid; 128695; 60.
DR   IntAct; Q96FN4; 16.
DR   MINT; MINT-8049255; -.
DR   STRING; 9606.ENSP00000290776; -.
DR   iPTMnet; Q96FN4; -.
DR   PhosphoSitePlus; Q96FN4; -.
DR   BioMuta; CPNE2; -.
DR   DMDM; 33860150; -.
DR   EPD; Q96FN4; -.
DR   MaxQB; Q96FN4; -.
DR   PaxDb; Q96FN4; -.
DR   PeptideAtlas; Q96FN4; -.
DR   PRIDE; Q96FN4; -.
DR   Ensembl; ENST00000290776; ENSP00000290776; ENSG00000140848. [Q96FN4-1]
DR   Ensembl; ENST00000535318; ENSP00000439018; ENSG00000140848. [Q96FN4-1]
DR   Ensembl; ENST00000565874; ENSP00000456042; ENSG00000140848. [Q96FN4-1]
DR   GeneID; 221184; -.
DR   KEGG; hsa:221184; -.
DR   UCSC; uc002eks.3; human. [Q96FN4-1]
DR   CTD; 221184; -.
DR   DisGeNET; 221184; -.
DR   GeneCards; CPNE2; -.
DR   HGNC; HGNC:2315; CPNE2.
DR   HPA; HPA041132; -.
DR   MIM; 604206; gene.
DR   neXtProt; NX_Q96FN4; -.
DR   OpenTargets; ENSG00000140848; -.
DR   PharmGKB; PA26832; -.
DR   eggNOG; KOG1327; Eukaryota.
DR   eggNOG; ENOG410XPC8; LUCA.
DR   GeneTree; ENSGT00760000119085; -.
DR   HOGENOM; HOG000220898; -.
DR   HOVERGEN; HBG066841; -.
DR   InParanoid; Q96FN4; -.
DR   OMA; MGPQYCV; -.
DR   OrthoDB; EOG091G05IT; -.
DR   PhylomeDB; Q96FN4; -.
DR   TreeFam; TF316419; -.
DR   BioCyc; ZFISH:ENSG00000140848-MONOMER; -.
DR   ChiTaRS; CPNE2; human.
DR   GenomeRNAi; 221184; -.
DR   PRO; PR:Q96FN4; -.
DR   Proteomes; UP000005640; Chromosome 16.
DR   Bgee; ENSG00000140848; -.
DR   CleanEx; HS_CPNE2; -.
DR   ExpressionAtlas; Q96FN4; baseline and differential.
DR   Genevisible; Q96FN4; HS.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0071277; P:cellular response to calcium ion; IEA:Ensembl.
DR   CDD; cd01459; vWA_copine_like; 1.
DR   Gene3D; 2.60.40.150; -; 2.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR010734; Copine.
DR   InterPro; IPR002035; VWF_A.
DR   Pfam; PF00168; C2; 2.
DR   Pfam; PF07002; Copine; 1.
DR   SMART; SM00239; C2; 2.
DR   SMART; SM00327; VWA; 1.
DR   SUPFAM; SSF49562; SSF49562; 2.
DR   SUPFAM; SSF53300; SSF53300; 1.
DR   PROSITE; PS50004; C2; 2.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell membrane; Complete proteome; Cytoplasm;
KW   Membrane; Nucleus; Reference proteome; Repeat.
FT   CHAIN         1    548       Copine-2.
FT                                /FTId=PRO_0000144836.
FT   DOMAIN       10    115       C2 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00041}.
FT   DOMAIN      141    246       C2 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00041}.
FT   DOMAIN      305    507       VWFA. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00219}.
FT   REGION      247    304       Linker region.
FT                                {ECO:0000250|UniProtKB:P59108}.
FT   VAR_SEQ       1    102       Missing (in isoform 2).
FT                                {ECO:0000303|Ref.2}.
FT                                /FTId=VSP_055536.
FT   CONFLICT    100    100       K -> E (in Ref. 5; CAH18411).
FT                                {ECO:0000305}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT       326    542       ipfam:Copine [T]
FT   MYHIT       305    507       ismart:VWA [T]
FT   MYHIT       161    246       iprf:C2 [T]
FT   MYHIT       155    261       ismart:C2 [T]
FT   MYHIT       162    254       ipfam:C2 [T]
FT   MYHIT        24    130       ismart:C2 [T]
FT   MYHIT        30    115       iprf:C2 [T]
FT   MYHIT        30    129       ipfam:C2 [T]
SQ   SEQUENCE   548 AA;  61190 MW;  39B962C82C591DA0 CRC64;
     MAHIPSGGAP AAGAAPMGPQ YCVCKVELSV SGQNLLDRDV TSKSDPFCVL FTENNGRWIE
     YDRTETAINN LNPAFSKKFV LDYHFEEVQK LKFALFDQDK SSMRLDEHDF LGQFSCSLGT
     IVSSKKITRP LLLLNDKPAG KGLITIAAQE LSDNRVITLS LAGRRLDKKD LFGKSDPFLE
     FYKPGDDGKW MLVHRTEVIK YTLDPVWKPF TVPLVSLCDG DMEKPIQVMC YDYDNDGGHD
     FIGEFQTSVS QMCEARDSVP LEFECINPKK QRKKKNYKNS GIIILRSCKI NRDYSFLDYI
     LGGCQLMFTV GIDFTASNGN PLDPSSLHYI NPMGTNEYLS AIWAVGQIIQ DYDSDKMFPA
     LGFGAQLPPD WKVSHEFAIN FNPTNPFCSG VDGIAQAYSA CLPHIRFYGP TNFSPIVNHV
     ARFAAQATQQ RTATQYFILL IITDGVISDM EETRHAVVQA SKLPMSIIIV GVGNADFAAM
     EFLDGDSRML RSHTGEEAAR DIVQFVPFRE FRNAAKETLA KAVLAELPQQ VVQYFKHKNL
     PPTNSEPA
//