ID CCD40_CHLRE Reviewed; 576 AA.
AC A8IQT2;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 17-FEB-2016, entry version 26.
DE RecName: Full=Coiled-coil domain-containing protein 40 homolog {ECO:0000305};
DE AltName: Full=Flagellar-associated protein 172 {ECO:0000303|PubMed:15998802};
GN Name=CCDC40; Synonyms=FAP172 {ECO:0000303|PubMed:15998802};
GN ORFNames=CHLREDRAFT_170513 {ECO:0000312|EMBL:EDP04735.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae;
OC Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055 {ECO:0000312|Proteomes:UP000006906};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503, and cw92;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K.,
RA Marechal-Drouard L., Marshall W.F., Qu L.H., Nelson D.R.,
RA Sanderfoot A.A., Spalding M.H., Kapitonov V.V., Ren Q., Ferris P.,
RA Lindquist E., Shapiro H., Lucas S.M., Grimwood J., Schmutz J.,
RA Cardol P., Cerutti H., Chanfreau G., Chen C.L., Cognat V., Croft M.T.,
RA Dent R., Dutcher S., Fernandez E., Fukuzawa H., Gonzalez-Ballester D.,
RA Gonzalez-Halphen D., Hallmann A., Hanikenne M., Hippler M., Inwood W.,
RA Jabbari K., Kalanon M., Kuras R., Lefebvre P.A., Lemaire S.D.,
RA Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L., Mittag M.,
RA Mittelmeier T., Moroney J.V., Moseley J., Napoli C., Nedelcu A.M.,
RA Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J., Purton S.,
RA Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L., Schroda M.,
RA Stern D., Umen J., Willows R., Wilson N., Zimmer S.L., Allmer J.,
RA Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P.,
RA Jorgensen R., Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T.,
RA Brokstein P., Dubchak I., Goodstein D., Hornick L., Huang Y.W.,
RA Jhaveri J., Luo Y., Martinez D., Ngau W.C., Otillar B., Poliakov A.,
RA Porter A., Szajkowski L., Werner G., Zhou K., Grigoriev I.V.,
RA Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and
RT plant functions.";
RL Science 318:245-250(2007).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=15998802; DOI=10.1083/jcb.200504008;
RA Pazour G.J., Agrin N., Leszyk J., Witman G.B.;
RT "Proteomic analysis of a eukaryotic cilium.";
RL J. Cell Biol. 170:103-113(2005).
RN [3]
RP METHYLATION AT ARG-246 AND ARG-523.
RX PubMed=24152136; DOI=10.1021/bi4011623;
RA Werner-Peterson R., Sloboda R.D.;
RT "Methylation of structural components of the axoneme occurs during
RT flagellar disassembly.";
RL Biochemistry 52:8501-8509(2013).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CCDC39/FAP59, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=25395538; DOI=10.1126/science.1260214;
RA Oda T., Yanagisawa H., Kamiya R., Kikkawa M.;
RT "Cilia and flagella. A molecular ruler determines the repeat length in
RT eukaryotic cilia and flagella.";
RL Science 346:857-860(2014).
CC -!- FUNCTION: Required for assembly of dynein regulatory complex (DRC)
CC and inner dynein arm complexes, which are responsible for ciliary
CC beat regulation, by acting as a molecular ruler that determines
CC the 96 nanometer (nm) repeat length and arrangements of components
CC in cilia and flagella (PubMed:25395538). Together with
CC CCDC39/FAP59 forms a 96-nm-long complex in flagella. This complex
CC does not act as a physical ruler, but rather act as a negative
CC regulator for radial spokes: the complex lays along specific
CC protofilaments, masking radial spoke binding sites and allowing
CC recruitment of inner dynein arm (IDA) and nexin-dynein regulatory
CC complexes (N-DRC) (PubMed:25395538).
CC {ECO:0000269|PubMed:25395538}.
CC -!- SUBUNIT: Interacts with CCDC39/FAP59.
CC {ECO:0000269|PubMed:25395538}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:25395538}.
CC -!- PTM: Asymmetrically dimethylated at Arg-246 and Arg-523 during
CC flagellum resorption. Probably methylated by PRMT1.
CC {ECO:0000269|PubMed:24152136}.
CC -!- DISRUPTION PHENOTYPE: Short and immotile flagella. Inner dynein
CC arm (IDA) and nexin-dynein regulatory complex (N-DRC) components
CC are absent or severely reduced. Radial spokes are attached to
CC doublet microtubules with an irregular periodicity of 32 nm
CC instead of 96 nm. {ECO:0000269|PubMed:25395538}.
CC -!- SIMILARITY: Belongs to the CCDC40 family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The length of things -
CC Issue 170 of June 2015;
CC URL="http://web.expasy.org/spotlight/back_issues/170/";
DR EMBL; DS496120; EDP04735.1; -; Genomic_DNA.
DR RefSeq; XP_001691627.1; XM_001691575.1.
DR DIP; DIP-61435N; -.
DR STRING; 3055.EDP04735; -.
DR PaxDb; A8IQT2; -.
DR EnsemblPlants; EDP04735; EDP04735; CHLREDRAFT_170513.
DR GeneID; 5717350; -.
DR Gramene; EDP04735; EDP04735; CHLREDRAFT_170513.
DR KEGG; cre:CHLREDRAFT_170513; -.
DR InParanoid; A8IQT2; -.
DR Proteomes; UP000006906; Unassembled WGS sequence.
DR GO; GO:0005930; C:axoneme; IBA:GO_Central.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR GO; GO:0035082; P:axoneme assembly; IBA:GO_Central.
DR GO; GO:0060287; P:epithelial cilium movement involved in determination of left/right asymmetry; IBA:GO_Central.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW Complete proteome; Flagellum; Methylation; Reference proteome.
FT CHAIN 1 576 Coiled-coil domain-containing protein 40
FT homolog.
FT /FTId=PRO_0000431956.
FT COILED 33 175 {ECO:0000255}.
FT COILED 232 269 {ECO:0000255}.
FT COILED 311 397 {ECO:0000255}.
FT MOD_RES 246 246 Asymmetric dimethylarginine.
FT {ECO:0000269|PubMed:24152136}.
FT MOD_RES 523 523 Asymmetric dimethylarginine.
FT {ECO:0000269|PubMed:24152136}.
SQ SEQUENCE 576 AA; 63599 MW; B24A1CC4E7F6B42D CRC64;
MADPMDQPST SDPVDNQIFG EQGGLRPDHP LLRRAQEALK VQFEANRTRL QEELREKANA
LKQAKARREA LGVELYGFQQ NLAKLQLNLE TTHQNYQCED QLNQLKQQLS LEEGDTKGER
SRRVCVCVCR VCCRIDTLQD NLKGTQQQLA LVSAQLEAQK RETRAALETL AEAEVGGCVR
DEALSAIQDG MREQQQQELS LVLEIEGYKK DVVREQLKHE SLTAVVRKVE GDAVFVQKQI
EGAQERQARL QEILAKLAKS LEHTEAEGEA DAVDRAITKV AAEGRAIEEE MLSALSDQTT
AEKATSKTAA DTQELRKRIR AEELAVVETE NELAKLQVDI LNTEAHNSRL GETLGLLDEE
LRDKGRTIEK YELEIKRRND EIEKKTREID ILNRRRDCRG SAALDTRPLQ APPPPQVKSD
LALTTPMYTP PPVPQPSVGM TVTTEKLVSD MEKALTKREI ISVKGRATAA KSKSSTPAGS
ATASSRASPS ASVASSTLTR NQLDRATTDL AKSIKDLEAG RYRPVVEDAA AVGEELGRAQ
DKLGRVVALL EGLRQAAPHL AGELDKVLCH VADVRA
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