Entry sw:BH100_ARATH

ID   BH100_ARATH             Reviewed;         242 AA.
AC   Q9ZVB5; Q3E7M6;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   30-NOV-2016, entry version 100.
DE   RecName: Full=Transcription factor bHLH100;
DE   AltName: Full=Basic helix-loop-helix protein 100;
DE            Short=AtbHLH100;
DE            Short=bHLH 100;
DE   AltName: Full=Transcription factor EN 7;
DE   AltName: Full=bHLH transcription factor bHLH100;
GN   Name=BHLH100; Synonyms=EN7; OrderedLocusNames=At2g41240;
GN   ORFNames=F13H10.21;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RL   Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 17-242 (ISOFORM 2), TISSUE SPECIFICITY,
RP   INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=12679534; DOI=10.1093/molbev/msg088;
RA   Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT   "The basic helix-loop-helix transcription factor family in plants: a
RT   genome-wide study of protein structure and functional diversity.";
RL   Mol. Biol. Evol. 20:735-747(2003).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=12897250; DOI=10.1105/tpc.013839;
RA   Toledo-Ortiz G., Huq E., Quail P.H.;
RT   "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL   Plant Cell 15:1749-1770(2003).
RN   [6]
RP   INDUCTION.
RX   PubMed=17516080; DOI=10.1007/s00425-007-0535-x;
RA   Wang H.-Y., Klatte M., Jakoby M., Baeumlein H., Weisshaar B.,
RA   Bauer P.;
RT   "Iron deficiency-mediated stress regulation of four subgroup Ib BHLH
RT   genes in Arabidopsis thaliana.";
RL   Planta 226:897-908(2007).
RN   [7]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=18088336; DOI=10.1111/j.1365-3040.2007.01764.x;
RA   van de Mortel J.E., Schat H., Moerland P.D., Ver Loren van Themaat E.,
RA   van der Ent S., Blankestijn H., Ghandilyan A., Tsiatsiani S.,
RA   Aarts M.G.M.;
RT   "Expression differences for genes involved in lignin, glutathione and
RT   sulphate metabolism in response to cadmium in Arabidopsis thaliana and
RT   the related Zn/Cd-hyperaccumulator Thlaspi caerulescens.";
RL   Plant Cell Environ. 31:301-324(2008).
CC   -!- FUNCTION: Plays a role in metal homeostasis. Confers tolerance to
CC       high zinc (Zn) and nickel (Ni). {ECO:0000269|PubMed:18088336}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-
CC       ProRule:PRU00981}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9ZVB5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9ZVB5-2; Sequence=VSP_036095;
CC         Note=May be due to a competing acceptor splice site. No
CC         experimental confirmation available.;
CC   -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and
CC       stems. {ECO:0000269|PubMed:12679534}.
CC   -!- INDUCTION: Up regulated by iron deficiency in roots and leaves, as
CC       well as by nickel, high zinc or high copper treatments. Repressed
CC       by heat treatment, high iron, low copper and low zinc treatments.
CC       {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:17516080,
CC       ECO:0000269|PubMed:18088336}.
CC   -!- SIMILARITY: Contains 1 bHLH (basic helix-loop-helix) domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00981}.
DR   EMBL; AC005662; AAC78547.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC09951.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC09952.1; -; Genomic_DNA.
DR   EMBL; AY074635; AAL69451.1; -; mRNA.
DR   EMBL; AF488626; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; E84839; E84839.
DR   RefSeq; NP_181657.1; NM_129689.2. [Q9ZVB5-1]
DR   RefSeq; NP_850349.1; NM_180018.1. [Q9ZVB5-2]
DR   UniGene; At.37027; -.
DR   ProteinModelPortal; Q9ZVB5; -.
DR   BioGrid; 4060; 1.
DR   STRING; 3702.AT2G41240.1; -.
DR   PaxDb; Q9ZVB5; -.
DR   EnsemblPlants; AT2G41240.1; AT2G41240.1; AT2G41240. [Q9ZVB5-1]
DR   GeneID; 818723; -.
DR   Gramene; AT2G41240.1; AT2G41240.1; AT2G41240.
DR   KEGG; ath:AT2G41240; -.
DR   TAIR; AT2G41240; -.
DR   eggNOG; ENOG410IY4E; Eukaryota.
DR   eggNOG; ENOG410YP85; LUCA.
DR   HOGENOM; HOG000238662; -.
DR   InParanoid; Q9ZVB5; -.
DR   OMA; CASQLSN; -.
DR   OrthoDB; EOG09360NDZ; -.
DR   PhylomeDB; Q9ZVB5; -.
DR   PRO; PR:Q9ZVB5; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9ZVB5; baseline and differential.
DR   GO; GO:0090575; C:RNA polymerase II transcription factor complex; IBA:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003700; F:transcription factor activity, sequence-specific DNA binding; ISS:TAIR.
DR   GO; GO:0010106; P:cellular response to iron ion starvation; IEP:TAIR.
DR   GO; GO:0055072; P:iron ion homeostasis; IGI:TAIR.
DR   GO; GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR   GO; GO:0009414; P:response to water deprivation; IEP:TAIR.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR015660; Achaete-scute-rel.
DR   InterPro; IPR011598; bHLH_dom.
DR   PANTHER; PTHR13935; PTHR13935; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; DNA-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN         1    242       Transcription factor bHLH100.
FT                                /FTId=PRO_0000358789.
FT   DOMAIN       61    113       bHLH. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00981}.
FT   VAR_SEQ      96     96       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:12679534}.
FT                                /FTId=VSP_036095.
FT   CONFLICT    215    215       Q -> R (in Ref. 4; AF488626).
FT                                {ECO:0000305}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT        61    113       iprf:BHLH [T]
FT   MYHIT        67    119       ismart:HLH [T]
FT   MYHIT        62    114       ipfam:HLH [T]
SQ   SEQUENCE   242 AA;  27217 MW;  2E3FDE4D3653C1E3 CRC64;
     MCALVPPLYP NFGWPCGDHS FYETDDVSNT FLDFPLPDLT VTHENVSSEN NRTLLDNPVV
     MKKLNHNASE RERRKKINTM FSSLRSCLPP TNQTKKLSVS ATVSQALKYI PELQEQVKKL
     MKKKEELSFQ ISGQRDLVYT DQNSKSEEGV TSYASTVSST RLSETEVMVQ ISSLQTEKCS
     FGNVLSGVEE DGLVLVGASS SRSHGERLFY SMHLQIKNGQ VNSEELGDRL LYLYEKCGHS
     FT
//