Legends: 1, Alphavirus-like MT. {ECO:0000255|PROSITE- ProRule:PRU01079}; 2, (+)RNA virus helicase ATP-binding; 3, (+)RNA virus helicase C-terminal; 4, RdRp catalytic. {ECO:0000255|PROSITE- ProRule:PRU00539}; 5, REGION Methyltransferase. {ECO:0000250}; 6, REGION Helicase. {ECO:0000250}; 7, COILED {ECO:0000255}; 8, COMPBIAS Poly-Leu; 9, iprf:PSRV_HELICASE [T]; 10, ipfam:Viral_helicase1 [T]; 11, iprf:ALPHAVIRUS_MT [T]; 12, iprf:RDRP_SSRNA_POS [T].
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ID 194K_TRVSY Reviewed; 1707 AA.
AC P05080;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 2.
DT 07-SEP-2016, entry version 82.
DE RecName: Full=Replicase large subunit;
DE EC=2.1.1.-;
DE EC=2.7.7.-;
DE EC=2.7.7.48;
DE EC=3.6.4.13;
DE AltName: Full=194 kDa protein;
DE AltName: Full=RNA-directed RNA polymerase;
DE Contains:
DE RecName: Full=Replicase small subunit;
DE EC=2.1.1.-;
DE EC=2.7.7.-;
DE EC=3.6.4.13;
DE AltName: Full=134 kDa protein;
DE AltName: Full=Methyltransferase/RNA helicase;
OS Tobacco rattle virus (strain SYM).
OC Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage;
OC Virgaviridae; Tobravirus.
OX NCBI_TaxID=12298;
OH NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH NCBI_TaxID=4072; Capsicum annuum (Bell pepper).
OH NCBI_TaxID=82024; Hyacinthus.
OH NCBI_TaxID=39639; Narcissus pseudonarcissus (Daffodil).
OH NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH NCBI_TaxID=13274; Stellaria media (Common chickweed) (Alsine media).
OH NCBI_TaxID=13305; Tulipa.
OH NCBI_TaxID=97415; Viola arvensis (European field pansy) (Field violet).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3668507; DOI=10.1099/0022-1317-68-10-2563;
RA Hamilton W.D.O., Boccara M., Robinson D.J., Baulcombe D.C.;
RT "The complete nucleotide sequence of tobacco rattle virus RNA-1.";
RL J. Gen. Virol. 68:2563-2575(1987).
RN [2]
RP PARTIAL NUCLEOTIDE SEQUENCE.
RX PubMed=16453668;
RA Boccara M., Hamilton W.D.O., Baulcombe D.C.;
RT "The organisation and interviral homologies of genes at the 3' end of
RT tobacco rattle virus RNA1.";
RL EMBO J. 5:223-229(1986).
CC -!- FUNCTION: Replicase large subunit: is an RNA-dependent RNA
CC polymerase active in viral RNA replication. {ECO:0000250}.
CC -!- FUNCTION: Replicase small subunit: is a methyltransferase active
CC in RNA capping and an RNA helicase. Methyltransferase displays a
CC cytoplasmic capping enzyme activity. This function is necessary
CC since all viral RNAs are synthesized in the cytoplasm, and host
CC capping enzymes are restricted to the nucleus. Helicase region
CC probably exhibits NTPase and RNA unwinding activities (Potential).
CC {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC + RNA(n+1). {ECO:0000255|PROSITE-ProRule:PRU00539}.
CC -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: The replicase large subunit is translated as a
CC fusion protein by episodic readthrough of a termination codon.
CC When readthrough of the terminator codon TGA occurs between the
CC codons for 1187-Lys and 1189-Arg, this results in the addition of
CC the RdRp region.
CC -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-
CC directed RNA polymerase family. {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 (+)RNA virus helicase ATP-binding domain.
CC {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 (+)RNA virus helicase C-terminal domain.
CC {ECO:0000305}.
CC -!- SIMILARITY: Contains 1 alphavirus-like MT (methyltransferase)
CC domain. {ECO:0000255|PROSITE-ProRule:PRU01079}.
CC -!- SIMILARITY: Contains 1 RdRp catalytic domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00539}.
DR EMBL; X06172; CAA29537.1; -; Genomic_RNA.
DR EMBL; D00155; BAA00110.1; -; Genomic_RNA.
DR PIR; S01865; S01865.
DR ProteinModelPortal; P05080; -.
DR PRIDE; P05080; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Helicase; Hydrolase; Methyltransferase;
KW Nucleotide-binding; Nucleotidyltransferase; RNA-binding;
KW RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT CHAIN 1 1707 Replicase large subunit.
FT /FTId=PRO_0000040215.
FT CHAIN 1 1187 Replicase small subunit. {ECO:0000250}.
FT /FTId=PRO_0000040216.
FT DOMAIN 86 300 Alphavirus-like MT. {ECO:0000255|PROSITE-
FT ProRule:PRU01079}.
FT DOMAIN 872 1033 (+)RNA virus helicase ATP-binding.
FT DOMAIN 1034 1187 (+)RNA virus helicase C-terminal.
FT DOMAIN 1449 1562 RdRp catalytic. {ECO:0000255|PROSITE-
FT ProRule:PRU00539}.
FT REGION 65 425 Methyltransferase. {ECO:0000250}.
FT REGION 901 1155 Helicase. {ECO:0000250}.
FT COILED 601 622 {ECO:0000255}.
FT COMPBIAS 107 110 Poly-Leu.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 872 1187 iprf:PSRV_HELICASE [T]
FT MYHIT 901 1155 ipfam:Viral_helicase1 [T]
FT MYHIT 86 300 iprf:ALPHAVIRUS_MT [T]
FT MYHIT 1449 1562 iprf:RDRP_SSRNA_POS [T]
FT MYHIT 1240 1677 ipfam:RdRP_2 [T]
FT MYHIT 66 448 ipfam:Vmethyltransf [T]
SQ SEQUENCE 1707 AA; 194330 MW; F4DABDB6C7F51F04 CRC64;
MANGNFKLSQ LLNVDEMSAE QRSHFFDLML TKPDCEIGQM MQRVVVDKVD DMIRERKTKD
PVIVHEVLSQ KEQNKLMEIY PEFNIVFKDD KNMVHGFAAA ERKLQALLLL DRVPALQEVD
DIGGQWSFWV TRGEKRIHSC CPNLDIRDDQ REISRQIFLT AIGDQARSGK RQMSENELWM
YDQFRKNIAA PNAVRCNNTY QGCTCRGFSD GKKKGAQYAI ALHSLYDFKL KDLMATMVEK
KTKVVHAAML FAPESMLVDE GPLPSVDGYY MKKNGKIYFG FEKDPSFSYI HDWEEYKKYL
LGKPVSYQGN VFYFEPWQVR GDTMLFSIYR IAGVPRRSLS SQEYYRRIYI SRWENMVVVP
IFDLVESTRE LVKKDLFVEK QFMDKCLDYI ARLSDQQLTI SNVKSYLSSN NWVLFINGAA
VKNKQSVDSR DLQLLAQTLL VKEQVARPVM RELREAILTE TKPITSLTDV LGLISRKLWK
QFANKIAVGG FVGMVGTLIG FYPKKVLTWA KDTPNGPELC YENSHKTKVI VFLSVVYAIG
GITLMRRDIR DGLVKKLCDM FDIKRGAHVL DVENPCRYYE INDFFSSLYS ASESGETVLP
DLSEVKAKSD KLLQQKKEIA DEFLSAKFSN YSGSSVRTSP PSVVGSSRSG LGLLLEDSNV
LTQARVGVSR KVDDEEIMEQ FLSGLIDTEA EIDEVVSAFS AECERGETSG TKVLCKPLTP
PGFENVLPAV KPLVSKGKTV KRVDYFQVMG GERLPKRPVV SGDNSVDARR EFLYYLDAER
VAQNDEIMSL YRDYSRGVIR TGGQNYPHGL GVWDVEMKNW CIRPVVTEHA YVFQPDKRMD
DWSGYLEVAV WERGMLVNDF AVERMSDYVI VCDQTYLCNN RLILDNLSAL DLGPVNCSFE
LVDGVPGCGK STMIVNSANP CVDVVLSTGR AATDDLIERF ASKGFPCKLK RRVKTVDSFL
MHCVDGSLTG DVLHFDEALM AHAGMVYFCA QIAGAKRCIC QGDQNQISFK PRVSQVDLRF
SSLVGKFDIV TEKRETYRSP ADVAAVLNKY YTGDVRTHNA TANSMTVRKI VSKEQVSLKP
GAQYITFLQS EKKELVNLLA LRKVAAKVST VHESQGETFK DVVLVRTKPT DDSIARGREY
LIVALSRHTQ SLVYETVKED DVSKEIRESA ALTKAALARF FVTETVLXRF RSRFDVFRHH
EGPCAVPDSG TITDLEMWYD ALFPGNSLRD SSLDGYLVAT TDCNLRLDNV TIKSGNWKDK
FAEKETFLKP VIRTAMPDKR KTTQLESLLA LQKRNQAAPD LQENVHATVL IEETMKKLKS
VVYDVGKIRA DPIVNRAQME RWWRNQSTAV QAKVVADVRE LHEIDYSSYM YMIKSDVKPK
TDLTPQFEYS ALQTVVYHEK LINSLFGPIF KEINERKLDA MQPHFVFNTR MTSSDLNDRV
KFLNTEAAYD FVEIDMSKFD KSANRFHLQL QLEIYRLFGL DEWAAFLWEV SHTQTTVRDI
QNGMMAHIWY QQKSGDADTY NANSDRTLCA LLSELPLEKA VMVTYGGDDS LIAFPRGTQF
VDPCPKLATK WNFECKIFKY DVPMFCGKFL LKTSSCYEFV PDPVKVLTKL GKKSIKDVQH
LAEIYISLND SNRALGNYMV VSKLSESVSD RYLYKGDSVH ALCALWKHIK SFTALCTLFR
DENDKELNPA KVDWKKAQRA VSNFYDW
//
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