Entry euk:PUS10_DROME

ID   PUS10_DROME             Reviewed;         500 AA.
AC   Q9VPK7;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 2.
DT   12-APR-2017, entry version 93.
DE   RecName: Full=Putative tRNA pseudouridine synthase Pus10;
DE            EC=5.4.99.25;
DE   AltName: Full=Coiled-coil domain-containing protein 139 homolog;
DE   AltName: Full=tRNA pseudouridine 55 synthase;
DE            Short=Psi55 synthase;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=Pus10; ORFNames=CG3709;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC   Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA   Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA   Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA   Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA   Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA   Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA   Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA   Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA   Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA   de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA   Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA   Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA   Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA   Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA   Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA   Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA   Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA   Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA   Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA   Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA   Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA   Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA   Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA   Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA   Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA   Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA   Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA   Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA   Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA   Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA   Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA   Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a
RT   systematic review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA   George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA   Rubin G.M., Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-
CC       55 in the psi GC loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55).
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000305}.
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DR   EMBL; AE014134; AAF51539.2; -; Genomic_DNA.
DR   EMBL; AY069446; AAL39591.1; -; mRNA.
DR   RefSeq; NP_608500.1; NM_134656.4.
DR   UniGene; Dm.2125; -.
DR   ProteinModelPortal; Q9VPK7; -.
DR   SMR; Q9VPK7; -.
DR   BioGrid; 59438; 2.
DR   IntAct; Q9VPK7; 1.
DR   STRING; 7227.FBpp0077806; -.
DR   PaxDb; Q9VPK7; -.
DR   PRIDE; Q9VPK7; -.
DR   EnsemblMetazoa; FBtr0078147; FBpp0077806; FBgn0031227.
DR   GeneID; 33178; -.
DR   KEGG; dme:Dmel_CG3709; -.
DR   UCSC; CG3709-RA; d. melanogaster.
DR   FlyBase; FBgn0031227; CG3709.
DR   eggNOG; KOG2364; Eukaryota.
DR   eggNOG; COG1258; LUCA.
DR   GeneTree; ENSGT00390000007529; -.
DR   InParanoid; Q9VPK7; -.
DR   KO; K07583; -.
DR   OMA; IKKVCQK; -.
DR   OrthoDB; EOG091G06K1; -.
DR   PhylomeDB; Q9VPK7; -.
DR   GenomeRNAi; 33178; -.
DR   PRO; PR:Q9VPK7; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0031227; -.
DR   Genevisible; Q9VPK7; DM.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Complete proteome; Isomerase; Reference proteome;
KW   tRNA processing.
FT   CHAIN         1    500       Putative tRNA pseudouridine synthase
FT                                Pus10.
FT                                /FTId=PRO_0000299025.
FT   REGION      267    280       RNA binding forefinger loop.
FT                                {ECO:0000255}.
FT   REGION      406    421       RNA binding thumb loop. {ECO:0000255}.
FT   COILED       30     50       {ECO:0000255}.
FT   ACT_SITE    309    309       Nucleophile. {ECO:0000255}.
FT   BINDING     378    378       Substrate. {ECO:0000255}.
FT   BINDING     451    451       Substrate. {ECO:0000255}.
SQ   SEQUENCE   500 AA;  56763 MW;  D0FA2B91035D78F7 CRC64;
     MKNQDLVEYL RSCGVCEICQ LRYLKARGAE YRNINETLQK LDVKLNENVE NEELVISEGS
     QPSKRARLGV CSTCLGLFSK DFQNELLSSI LASDFAKYDC QKIVLAICLP MSLQLRQLAM
     WFALRRRFGP SIDENNPPDV PIKEAVKLIL HPIICEKLAK VYDANGLMIN IDLTHSLEGE
     EVEKLVKLNK EAFPAKASHQ KRIEISRGLL EKQYQPSKVK AETYEKYFQI PCSNVEESIK
     LTSIDLQGPL ICVAGRYRKL SRELSHTPWI LNGQRLMEDS IEEIIIRHVG PHFTEKLGKI
     TFMSSGREDV DVRCLGKGRP FVLEIPNAKR SCLTKDEAFA MERAVDASGM VSIHHLQVVP
     REELTHIKTG EEQKKKFYRA LCALQEPVSV KILEQLQISA SFNIQQKTPI RVLHRRPLHT
     RPRTIYSVTA KVHRGNPKAL IIDIVTQAGT YIKELVHGEF GRTTPSLSSI IGKPMDIQAL
     DVVGIDLDWP HEVDNSKHKE
//