ID MYB80_ARATH Reviewed; 320 AA.
AC Q9XHV0;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 10-MAY-2017, entry version 139.
DE RecName: Full=Transcription factor MYB80 {ECO:0000305};
DE AltName: Full=MYB-related protein 103 {ECO:0000303|PubMed:10353220};
DE Short=AtMYB103 {ECO:0000303|PubMed:10353220};
DE AltName: Full=Myb-related protein 80 {ECO:0000305};
DE Short=AtMYB80 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Protein MALE STERILE 188 {ECO:0000303|PubMed:17727613};
GN Name=MYB80 {ECO:0000303|PubMed:11597504};
GN Synonyms=MS188 {ECO:0000303|PubMed:17727613},
GN MYB103 {ECO:0000303|PubMed:10353220};
GN OrderedLocusNames=At5g56110 {ECO:0000312|Araport:AT5G56110};
GN ORFNames=MDA7.17 {ECO:0000312|EMBL:BAB09293.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10353220; DOI=10.1093/oxfordjournals.pcp.a029548;
RA Li S.F., Higginson T., Parish R.W.;
RT "A novel MYB-related gene from Arabidopsis thaliana expressed in
RT developing anthers.";
RL Plant Cell Physiol. 40:343-347(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide
RT cloning and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one
RT physically assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RG The Arabidopsis Information Portal (Araport);
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N.,
RA Takiguchi Y., Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/S1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12848824; DOI=10.1046/j.1365-313X.2003.01791.x;
RA Higginson T., Li S.F., Parish R.W.;
RT "AtMYB103 regulates tapetum and trichome development in Arabidopsis
RT thaliana.";
RL Plant J. 35:177-192(2003).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS
RP OF PRO-41.
RX PubMed=17727613; DOI=10.1111/j.1365-313X.2007.03254.x;
RA Zhang Z.B., Zhu J., Gao J.F., Wang C., Li H., Li H., Zhang H.Q.,
RA Zhang S., Wang D.M., Wang Q.X., Huang H., Xia H.J., Yang Z.N.;
RT "Transcription factor AtMYB103 is required for anther development by
RT regulating tapetum development, callose dissolution and exine
RT formation in Arabidopsis.";
RL Plant J. 52:528-538(2007).
RN [9]
RP DEVELOPMENTAL STAGE.
RX PubMed=21957980; DOI=10.1111/j.1744-7909.2011.01078.x;
RA Zhu J., Lou Y., Xu X., Yang Z.N.;
RT "A genetic pathway for tapetum development and function in
RT Arabidopsis.";
RL J. Integr. Plant Biol. 53:892-900(2011).
RN [10]
RP FUNCTION.
RX PubMed=21673079; DOI=10.1105/tpc.110.082651;
RA Phan H.A., Iacuone S., Li S.F., Parish R.W.;
RT "The MYB80 transcription factor is required for pollen development and
RT the regulation of tapetal programmed cell death in Arabidopsis
RT thaliana.";
RL Plant Cell 23:2209-2224(2011).
CC -!- FUNCTION: Transcription factor that binds to the DNA sequence 5'-
CC CCAACC-3'. Regulates directly PME5, UND and GLOX1
CC (PubMed:21673079). Essential for tapetum development in anthers
CC and microsporogenesis (PubMed:12848824, PubMed:21673079).
CC Regulates the timing of tapetal programmed cell death (PCD) which
CC is critical for pollen development. May act through the activation
CC of UND, encoding an A1 aspartic protease (PubMed:21673079).
CC Required for anther development by regulating tapetum development,
CC callose dissolution and exine formation. Acts upstream of A6 and
CC FAR2/MS2, two genes required for pollen exine formation
CC (PubMed:17727613). Negatively regulates trichome endoreduplication
CC and trichome branching (PubMed:12848824).
CC {ECO:0000269|PubMed:12848824, ECO:0000269|PubMed:17727613,
CC ECO:0000269|PubMed:21673079}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-
CC ProRule:PRU00625, ECO:0000269|PubMed:17727613}.
CC -!- TISSUE SPECIFICITY: Expressed in the tapetum and middle layer of
CC developing anthers (PubMed:10353220). Expressed in trichomes
CC (PubMed:12848824). {ECO:0000269|PubMed:10353220,
CC ECO:0000269|PubMed:12848824}.
CC -!- DEVELOPMENTAL STAGE: During anther development, expressed from
CC stage 4 to stage 6 in microsporocytes and tapetal cells. At the
CC tetrad stage, expressed predominantly in tapetal cells. During
CC microgametogenesis, expression decreases radically in the tapetum
CC and microspores. {ECO:0000269|PubMed:21957980}.
CC -!- DISRUPTION PHENOTYPE: Male sterility due to distorted pollen
CC grains lacking reticulate exine on their surface.
CC {ECO:0000269|PubMed:17727613}.
CC -!- MISCELLANEOUS: Plants silencing MYB80 have the majority of pollen
CC grains distorted in shape with reduced or no cytoplasmic content,
CC display early tapetal degeneration with large opaque bodies in the
CC tapetal cytoplasm, and trichomes on cauline and rosette leaves
CC contain increased nuclear DNA content and produces additional
CC branches. {ECO:0000269|PubMed:12848824}.
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DR EMBL; AF048839; AAD40692.1; -; Genomic_DNA.
DR EMBL; AY519639; AAS10109.1; -; mRNA.
DR EMBL; AB011476; BAB09293.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96722.1; -; Genomic_DNA.
DR EMBL; AB493793; BAH30631.1; -; mRNA.
DR RefSeq; NP_200422.1; NM_124993.1.
DR UniGene; At.50533; -.
DR ProteinModelPortal; Q9XHV0; -.
DR SMR; Q9XHV0; -.
DR STRING; 3702.AT5G56110.1; -.
DR PaxDb; Q9XHV0; -.
DR EnsemblPlants; AT5G56110.1; AT5G56110.1; AT5G56110.
DR GeneID; 835710; -.
DR Gramene; AT5G56110.1; AT5G56110.1; AT5G56110.
DR KEGG; ath:AT5G56110; -.
DR Araport; AT5G56110; -.
DR TAIR; locus:2161820; AT5G56110.
DR eggNOG; KOG0048; Eukaryota.
DR eggNOG; COG5147; LUCA.
DR HOGENOM; HOG000237600; -.
DR KO; K09422; -.
DR OMA; NQINFSN; -.
DR OrthoDB; EOG09360I9R; -.
DR PRO; PR:Q9XHV0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9XHV0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003682; F:chromatin binding; IDA:TAIR.
DR GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0001135; F:transcription factor activity, RNA polymerase II transcription factor recruiting; IBA:GO_Central.
DR GO; GO:0003700; F:transcription factor activity, sequence-specific DNA binding; ISS:TAIR.
DR GO; GO:0044212; F:transcription regulatory region DNA binding; IBA:GO_Central.
DR GO; GO:0048658; P:anther wall tapetum development; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR GO; GO:0010090; P:trichome morphogenesis; IMP:TAIR.
DR InterPro; IPR009057; Homeobox-like.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Complete proteome; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1 320 Transcription factor MYB80.
FT /FTId=PRO_0000436452.
FT DOMAIN 9 65 HTH myb-type 1. {ECO:0000255|PROSITE-
FT ProRule:PRU00625}.
FT DOMAIN 66 116 HTH myb-type 2. {ECO:0000255|PROSITE-
FT ProRule:PRU00625}.
FT DNA_BIND 37 61 H-T-H motif. {ECO:0000255|PROSITE-
FT ProRule:PRU00625}.
FT DNA_BIND 89 112 H-T-H motif. {ECO:0000255|PROSITE-
FT ProRule:PRU00625}.
FT COMPBIAS 294 299 His-rich. {ECO:0000255|PROSITE-
FT ProRule:PRU00009}.
FT MUTAGEN 41 41 P->L: In ms188-2; male sterility.
FT {ECO:0000269|PubMed:17727613}.
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 13 63 ismart:SANT [T]
FT MYHIT 66 116 iprf:HTH_MYB [T]
FT MYHIT 14 61 ipfam:Myb_DNA-binding [T]
FT MYHIT 66 114 ismart:SANT [T]
FT MYHIT 9 65 iprf:HTH_MYB [T]
FT MYHIT 68 110 ipfam:Myb_DNA-binding [T]
SQ SEQUENCE 320 AA; 35983 MW; A6333EE5D22269CF CRC64;
MGRIPCCEKE NVKRGQWTPE EDNKLASYIA QHGTRNWRLI PKNAGLQRCG KSCRLRWTNY
LRPDLKHGQF SEAEEHIIVK FHSVLGNRWS LIAAQLPGRT DNDVKNYWNT KLKKKLSGMG
IDPVTHKPFS HLMAEITTTL NPPQVSHLAE AALGCFKDEM LHLLTKKRVD LNQINFSNHN
PNPNNFHEIA DNEAGKIKMD GLDHGNGIMK LWDMGNGFSY GSSSSSFGNE ERNDGSASPA
VAAWRGHGGI RTAVAETAAA EEEERRKLKG EVVDQEEIGS EGGRGDGMTM MRNHHHHQHV
FNVDNVLWDL QADDLINHMV
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