ID YQHD_ECOLI Reviewed; 387 AA.
AC Q46856; Q2M9I8;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-NOV-2009, entry version 62.
DE RecName: Full=Alcohol dehydrogenase yqhD;
DE EC=1.1.1.-;
GN Name=yqhD; OrderedLocusNames=b3011, JW2978;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-387 IN COMPLEX WITH ZINC
RP IONS AND NADP, FUNCTION, AND SUBUNIT.
RX PubMed=15327949; DOI=10.1016/j.jmb.2004.07.034;
RA Sulzenbacher G., Alvarez K., Van Den Heuvel R.H., Versluis C.,
RA Spinelli S., Campanacci V., Valencia C., Cambillau C., Eklund H.,
RA Tegoni M.;
RT "Crystal structure of E.coli alcohol dehydrogenase YqhD: evidence of a
RT covalently modified NADP coenzyme.";
RL J. Mol. Biol. 342:489-502(2004).
CC -!- FUNCTION: NADP-dependent ADH activity.
CC -!- CATALYTIC ACTIVITY: An alcohol + NADP(+) = an aldehyde + NADPH.
CC -!- COFACTOR: Binds 1 zinc ion per subunit.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC family.
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DR EMBL; U28377; AAA69178.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76047.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77068.1; -; Genomic_DNA.
DR PIR; A65088; A65088.
DR RefSeq; AP_003562.1; -.
DR RefSeq; NP_417484.1; -.
DR PDB; 1OJ7; X-ray; 2.00 A; A/B/C/D=2-387.
DR PDBsum; 1OJ7; -.
DR IntAct; Q46856; 3.
DR STRING; Q46856; -.
DR GeneID; 947493; -.
DR GenomeReviews; AP009048_GR; JW2978.
DR GenomeReviews; U00096_GR; b3011.
DR KEGG; ecj:JW2978; -.
DR KEGG; eco:b3011; -.
DR EchoBASE; EB2834; -.
DR EcoGene; EG13014; yqhD.
DR HOGENOM; Q46856; -.
DR OMA; HDWATHM; -.
DR BioCyc; EcoCyc:G7564-MON; -.
DR BioCyc; MetaCyc:G7564-MON; -.
DR Genevestigator; Q46856; -.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR InterPro; IPR001670; ADH_Fe.
DR InterPro; IPR018211; ADH_Fe_CS.
DR Pfam; PF00465; Fe-ADH; 1.
DR PROSITE; PS00913; ADH_IRON_1; 1.
DR PROSITE; PS00060; ADH_IRON_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Metal-binding; NADP; Oxidoreductase;
KW Zinc.
FT CHAIN 1 387 Alcohol dehydrogenase yqhD.
FT /FTId=PRO_0000087832.
FT NP_BIND 38 40 NADP.
FT NP_BIND 93 99 NADP.
FT NP_BIND 179 182 NADP.
FT METAL 194 194 Zinc; catalytic.
FT METAL 198 198 Zinc; catalytic.
FT METAL 267 267 Zinc; catalytic.
FT METAL 281 281 Zinc; catalytic.
FT BINDING 138 138 NADP.
FT BINDING 147 147 NADP.
FT BINDING 160 160 NADP.
FT STRAND 4 15
FT HELIX 18 21
FT HELIX 22 25
FT STRAND 31 36
FT HELIX 40 44
FT HELIX 46 53
FT TURN 54 56
FT STRAND 57 62
FT HELIX 71 84
FT STRAND 88 94
FT HELIX 95 107
FT HELIX 118 121
FT TURN 122 126
FT STRAND 133 139
FT HELIX 144 146
FT STRAND 147 154
FT TURN 155 158
FT STRAND 159 164
FT HELIX 166 168
FT STRAND 171 175
FT HELIX 177 180
FT HELIX 185 203
FT STRAND 204 206
FT HELIX 211 233
FT HELIX 238 251
FT STRAND 252 254
FT TURN 255 259
FT HELIX 265 277
FT HELIX 281 295
FT TURN 296 300
FT HELIX 301 312
FT HELIX 319 336
FT HELIX 343 346
FT HELIX 353 362
FT STRAND 366 368
FT TURN 369 372
FT HELIX 376 385
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 9 360 ipfam:Fe-ADH [T]
FT MYHIT 264 284 ipat:ADH_IRON_2 [T]
FT MYHIT 173 201 ipat:ADH_IRON_1 [T]
SQ SEQUENCE 387 AA; 42097 MW; 448A7610787D2146 CRC64;
MNNFNLHTPT RILFGKGAIA GLREQIPHDA RVLITYGGGS VKKTGVLDQV LDALKGMDVL
EFGGIEPNPA YETLMNAVKL VREQKVTFLL AVGGGSVLDG TKFIAAAANY PENIDPWHIL
QTGGKEIKSA IPMGCVLTLP ATGSESNAGA VISRKTTGDK QAFHSAHVQP VFAVLDPVYT
YTLPPRQVAN GVVDAFVHTV EQYVTKPVDA KIQDRFAEGI LLTLIEDGPK ALKEPENYDV
RANVMWAATQ ALNGLIGAGV PQDWATHMLG HELTAMHGLD HAQTLAIVLP ALWNEKRDTK
RAKLLQYAER VWNITEGSDD ERIDAAIAAT RNFFEQLGVP THLSDYGLDG SSIPALLKKL
EEHGMTQLGE NHDITLDVSR RIYEAAR
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