ID TOLC_ECOLI Reviewed; 493 AA.
AC P02930; Q2M9G5;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 3.
DT 03-NOV-2009, entry version 105.
DE RecName: Full=Outer membrane protein tolC;
DE Flags: Precursor;
GN Name=tolC; Synonyms=mtcB, mukA, refI; OrderedLocusNames=b3035, JW5503;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=84015386; PubMed=6312426; DOI=10.1093/nar/11.18.6487;
RA Hackett J., Reeves P.;
RT "Primary structure of the tolC gene that codes for an outer membrane
RT protein of Escherichia coli K12.";
RL Nucleic Acids Res. 11:6487-6495(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=91016844; PubMed=2216730; DOI=10.1093/nar/18.18.5547;
RA Niki H., Imamura R., Ogura T., Hiraga S.;
RT "Nucleotide sequence of the tolC gene of Escherichia coli.";
RL Nucleic Acids Res. 18:5547-5547(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
RC STRAIN=K12;
RX MEDLINE=83210228; PubMed=6303857; DOI=10.1016/0014-5793(83)80518-3;
RA Hackett J., Misra R., Reeves P.;
RT "The TolC protein of Escherichia coli K12 is synthesised in a
RT precursor form.";
RL FEBS Lett. 156:307-310(1983).
RN [6]
RP PROTEIN SEQUENCE OF 23-34.
RC STRAIN=K12 / EMG2;
RX MEDLINE=97443975; PubMed=9298646; DOI=10.1002/elps.1150180807;
RA Link A.J., Robison K., Church G.M.;
RT "Comparing the predicted and observed properties of proteins encoded
RT in the genome of Escherichia coli K-12.";
RL Electrophoresis 18:1259-1313(1997).
RN [7]
RP PROTEIN SEQUENCE OF 23-27.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX MEDLINE=98291876; PubMed=9629924; DOI=10.1002/elps.1150190539;
RA Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M.,
RA Sanchez J.-C., Hochstrasser D.F., Williams K.L., Gooley A.A.;
RT "Extraction of membrane proteins by differential solubilization for
RT separation using two-dimensional gel electrophoresis.";
RL Electrophoresis 19:837-844(1998).
RN [8]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=BL21-DE3;
RX PubMed=16079137; DOI=10.1074/jbc.M506479200;
RA Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L.,
RA von Heijne G., Daley D.O.;
RT "Protein complexes of the Escherichia coli cell envelope.";
RL J. Biol. Chem. 280:34409-34419(2005).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-450.
RX MEDLINE=20335872; PubMed=10879525; DOI=10.1038/35016007;
RA Koronakis V., Sharff A., Koronakis E., Luisi B., Hughes C.;
RT "Crystal structure of the bacterial membrane protein TolC central to
RT multidrug efflux and protein export.";
RL Nature 405:914-919(2000).
CC -!- FUNCTION: Required for proper expression of outer membrane protein
CC genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or
CC colicin E1. May be specialized for signal sequence independent,
CC extracellular secretion in Gram-negative bacteria.
CC -!- SUBUNIT: Homotrimer that assembles to form a continuous, solvent-
CC accessible conduit: a 'channel-tunnel' over 140 Angstroms long
CC that spans both the outer membrane and periplasmic space. The
CC periplasmic or proximal end of the tunnel is sealed by sets of
CC coiled helices.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC family.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA24914.1; Type=Miscellaneous discrepancy;
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DR EMBL; X00016; CAA24914.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X54049; CAA37982.1; ALT_INIT; Genomic_DNA.
DR EMBL; U28377; AAA69203.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76071.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77091.1; -; Genomic_DNA.
DR EMBL; V01505; CAA24751.1; ALT_INIT; Genomic_DNA.
DR PIR; A65091; MMECTC.
DR RefSeq; AP_003585.1; -.
DR RefSeq; NP_417507.2; -.
DR PDB; 1EK9; X-ray; 2.10 A; A/B/C=23-450.
DR PDB; 1TQQ; X-ray; 2.75 A; A/B/C=23-493.
DR PDB; 1TTQ; X-ray; 2.00 A; A=-, B=1-12.
DR PDB; 2VDD; X-ray; 3.30 A; A/B/C=3-450.
DR PDB; 2VDE; X-ray; 3.20 A; A/B/C=3-450.
DR PDBsum; 1EK9; -.
DR PDBsum; 1TQQ; -.
DR PDBsum; 1TTQ; -.
DR PDBsum; 2VDD; -.
DR PDBsum; 2VDE; -.
DR DIP; DIP:11007N; -.
DR IntAct; P02930; 2.
DR STRING; P02930; -.
DR TCDB; 1.B.17.1.1; outer membrane factor (OMF) family.
DR SWISS-2DPAGE; P02930; -.
DR 2DBase-Ecoli; P02930; -.
DR GeneID; 947521; -.
DR GenomeReviews; AP009048_GR; JW5503.
DR GenomeReviews; U00096_GR; b3035.
DR KEGG; ecj:JW5503; -.
DR KEGG; eco:b3035; -.
DR EchoBASE; EB1002; -.
DR EcoGene; EG11009; tolC.
DR HOGENOM; P02930; -.
DR OMA; AMSQAEN; -.
DR BioCyc; EcoCyc:EG11009-MON; -.
DR Genevestigator; P02930; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0005215; F:transporter activity; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR InterPro; IPR003423; OMP_efflux.
DR InterPro; IPR010130; T1SS_OMP_TolC.
DR Pfam; PF02321; OEP; 2.
DR TIGRFAMs; TIGR01844; type_I_sec_TolC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Cell outer membrane; Complete proteome;
KW Direct protein sequencing; Membrane; Repeat; Signal; Transmembrane;
KW Transport.
FT SIGNAL 1 22
FT CHAIN 23 493 Outer membrane protein tolC.
FT /FTId=PRO_0000013352.
FT TOPO_DOM 23 62 Periplasmic.
FT TRANSMEM 63 74 S1.
FT TOPO_DOM 75 82 Extracellular.
FT TRANSMEM 83 96 S2.
FT TOPO_DOM 97 268 Periplasmic.
FT TRANSMEM 269 279 S4.
FT TOPO_DOM 280 300 Extracellular.
FT TRANSMEM 301 311 S5.
FT TOPO_DOM 312 493 Periplasmic.
FT REPEAT 23 230 1.
FT REPEAT 231 446 2.
FT CONFLICT 191 191 V -> L (in Ref. 1; CAA37982).
FT HELIX 25 35
FT HELIX 37 57
FT HELIX 58 60
FT STRAND 63 75
FT STRAND 77 79
FT STRAND 83 98
FT HELIX 100 167
FT HELIX 173 208
FT STRAND 213 218
FT TURN 220 222
FT HELIX 231 241
FT HELIX 243 263
FT HELIX 264 266
FT STRAND 269 279
FT STRAND 282 285
FT STRAND 288 291
FT STRAND 302 316
FT HELIX 319 385
FT HELIX 391 426
FT HELIX 431 439
FT STRAND 441 448
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 27 209 ipfam:OEP [T]
FT MYHIT 232 427 ipfam:OEP [T]
SQ SEQUENCE 493 AA; 53741 MW; 6F97B4C62A848FE1 CRC64;
MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF EKINEARSPL
LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM SKWRALTLQE KAAGIQDVTY
QTDQQTLILN TATAYFNVLN AIDVLSYTQA QKEAIYRQLD QTTQRFNVGL VAITDVQNAR
AQYDTVLANE VTARNNLDNA VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK
RNLSLLQARL SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN
MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR SSFNNINASI
SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT LYNAKQELAN ARYNYLINQL
NIKSALGTLN EQDLLALNNA LSKPVSTNPE NVAPQTPEQN AIADGYAPDS PAPVVQQTSA
RTTTSNGHNP FRN
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