Entry ecoli:PTHC_ECOLI

ID   PTHC_ECOLI              Reviewed;         187 AA.
AC   P56579; P05705; P78102; P78214;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 1.
DT   03-NOV-2009, entry version 71.
DE   RecName: Full=Glucitol/sorbitol permease IIC component;
DE   AltName: Full=PTS system glucitol/sorbitol-specific EIIC component;
DE   AltName: Full=EIIC-Gut;
GN   Name=srlA; Synonyms=gutA, sbl; OrderedLocusNames=b2702, JW5429;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=87194727; PubMed=3553176;
RA   Yamada M., Saier M.H. Jr.;
RT   "Glucitol-specific enzymes of the phosphotransferase system in
RT   Escherichia coli. Nucleotide sequence of the gut operon.";
RL   J. Biol. Chem. 262:5455-5463(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RA   Reizer J., Reizer A., Yamada M., Saier M.H. Jr.;
RT   "The glucitol permease of Escherichia coli: a tripartite permease of
RT   the phosphotransferase system.";
RL   Microbiology 144:1463-1464(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   MEDLINE=97349980; PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K.,
RA   Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N.,
RA   Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H.,
RA   Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S.,
RA   Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C.,
RA   Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-
RT   K12 genome corresponding to 50.0-68.8 min on the linkage map and
RT   analysis of its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [5]
RP   SEQUENCE REVISION TO 21.
RX   PubMed=16397293; DOI=10.1093/nar/gkj405;
RA   Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA   Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA   Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H.,
RA   Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.;
RT   "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT   -- 2005.";
RL   Nucleic Acids Res. 34:1-9(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
RC   STRAIN=K12;
RX   MEDLINE=93096050; PubMed=1334233; DOI=10.1038/360606a0;
RA   Wang M.X., Church G.M.;
RT   "A whole genome approach to in vivo DNA-protein interactions in E.
RT   coli.";
RL   Nature 360:606-610(1992).
RN   [8]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (PTS), a major carbohydrate active
CC       -transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitant with their translocation across the cell
CC       membrane. This system is involved in glucitol/sorbitol transport.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC       protein.
CC   -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC       and contains the specific substrate-binding site.
CC   -!- SIMILARITY: Contains 1 PTS EIIC type-5 domain.
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DR   EMBL; J02708; AAC13411.1; -; Genomic_DNA.
DR   EMBL; U00096; AAT48148.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA18886.2; -; Genomic_DNA.
DR   EMBL; X70016; CAA49614.1; -; Genomic_DNA.
DR   PIR; A26725; WQEC2S.
DR   RefSeq; AP_003269.1; -.
DR   RefSeq; YP_026180.1; -.
DR   STRING; P56579; -.
DR   TCDB; 4.A.4.1.1; PTS glucitol (Gut) family.
DR   GeneID; 947575; -.
DR   GenomeReviews; AP009048_GR; JW5429.
DR   GenomeReviews; U00096_GR; b2702.
DR   KEGG; ecj:JW5429; -.
DR   KEGG; eco:b2702; -.
DR   EchoBASE; EB0962; -.
DR   EcoGene; EG10969; srlA.
DR   HOGENOM; P56579; -.
DR   OMA; FFRGWIT; -.
DR   BioCyc; EcoCyc:G8210-MON; -.
DR   Genevestigator; P56579; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phospho...; IEA:UniProtKB-KW.
DR   InterPro; IPR004699; PTS_IID_sorb.
DR   Pfam; PF03608; EII-GUT; 1.
DR   TIGRFAMs; TIGR00821; EII-GUT; 1.
DR   PROSITE; PS51107; PTS_EIIC_TYPE_5; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Complete proteome; Membrane;
KW   Phosphotransferase system; Sugar transport; Transmembrane; Transport.
FT   CHAIN         1    187       Glucitol/sorbitol permease IIC component.
FT                                /FTId=PRO_0000186565.
FT   TOPO_DOM      1     28       Periplasmic (Potential).
FT   TRANSMEM     29     49       Potential.
FT   TOPO_DOM     50     67       Cytoplasmic (Potential).
FT   TRANSMEM     68     88       Potential.
FT   TOPO_DOM     89    131       Periplasmic (Potential).
FT   TRANSMEM    132    152       Potential.
FT   TOPO_DOM    153    187       Cytoplasmic (Potential).
FT   DOMAIN        1    187       PTS EIIC type-5.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT         5    171       ipfam:EII-GUT [T]
FT   MYHIT         1    187       iprf:PTS_EIIC_TYPE_5 [T]
SQ   SEQUENCE   187 AA;  20580 MW;  86A10715BD9DE287 CRC64;
     MIETITHGAE WFIGLFQKGG EVFTGMVTGI LPLLISLLVI MNALINFIGQ HRIERFAQRC
     AGNPVSRYLL LPCIGTFVFC NPMTLSLGRF MPEKYKPSYY AAASYSCHSM NGLFPHINPG
     ELFVYLGIAS GLTTLNLPLG PLAVSYLLVG LVTNFFRGWV TDLTTAIFEK KMGIQLEQKV
     HLAGATS
//