Entry ecoli:GYRI_ECOLI

ID   GYRI_ECOLI              Reviewed;         157 AA.
AC   P33012;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-NOV-2009, entry version 76.
DE   RecName: Full=DNA gyrase inhibitory protein;
DE   AltName: Full=Protein sbmC;
GN   Name=gyrI; Synonyms=sbmC, yeeB; OrderedLocusNames=b2009, JW1991;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RYC1000;
RX   MEDLINE=96296452; PubMed=8709849;
RX   DOI=10.1111/j.1365-2958.1995.mmi_18020301.x;
RA   Baquero M.-R., Bouzon M., Varea J., Moreno F.;
RT   "sbmC, a stationary-phase induced SOS Escherichia coli gene, whose
RT   product protects cells from the DNA replication inhibitor microcin
RT   B17.";
RL   Mol. Microbiol. 18:301-311(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / BHB2600;
RA   Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA   Church G.M.;
RT   "Automated multiplex sequencing of the E.coli genome.";
RL   Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA   Yamamoto Y., Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   PROTEIN SEQUENCE OF 1-16, AND CHARACTERIZATION.
RX   MEDLINE=98113149; PubMed=9442027; DOI=10.1074/jbc.273.4.1933;
RA   Nakanishi A., Oshida T., Matsushita T., Imajoh-Ohmi S., Ohnuki T.;
RT   "Identification of DNA gyrase inhibitor (GyrI) in Escherichia coli.";
RL   J. Biol. Chem. 273:1933-1938(1998).
CC   -!- FUNCTION: Inhibits the supercoiling activity of DNA gyrase.
CC       Protects cells from the DNA replication inhibitor microcin B17
CC       (MccB17). Blocks MccB17 export from producing cells.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- INDUCTION: By DNA-damaging agents and by the entry of cells into
CC       the stationary growth phase.
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DR   EMBL; X84885; CAA59312.1; -; Genomic_DNA.
DR   EMBL; U00009; AAA16415.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75070.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15830.1; -; Genomic_DNA.
DR   PIR; H64965; H64965.
DR   RefSeq; AP_002609.1; -.
DR   RefSeq; NP_416513.1; -.
DR   PDB; 1JYH; X-ray; 1.80 A; A=1-157.
DR   PDBsum; 1JYH; -.
DR   DIP; DIP:9862N; -.
DR   STRING; P33012; -.
DR   GeneID; 946546; -.
DR   GenomeReviews; AP009048_GR; JW1991.
DR   GenomeReviews; U00096_GR; b2009.
DR   KEGG; ecj:JW1991; -.
DR   KEGG; eco:b2009; -.
DR   EchoBASE; EB1838; -.
DR   EcoGene; EG11892; sbmC.
DR   HOGENOM; P33012; -.
DR   OMA; NPDEVPA; -.
DR   BioCyc; EcoCyc:EG11892-MON; -.
DR   Genevestigator; P33012; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR010499; AraC_E_bd.
DR   Pfam; PF06445; AraC_E_bind; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing.
FT   CHAIN         1    157       DNA gyrase inhibitory protein.
FT                                /FTId=PRO_0000083882.
FT   STRAND        4      8
FT   STRAND       11     20
FT   HELIX        22     39
FT   STRAND       45     51
FT   TURN         55     57
FT   HELIX        60     62
FT   STRAND       64     71
FT   STRAND       85     89
FT   STRAND       92    101
FT   HELIX       107    117
FT   STRAND      121    125
FT   STRAND      130    134
FT   HELIX       138    141
FT   STRAND      142    154
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CC   The following FT lines are automated annotations from the MyHits database.
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FT   MYHIT         1    155       ihamap:DNA_gyrase_inhibitor [T]
FT   MYHIT         1    152       ipfam:GyrI-like [T]
FT   MYHIT         1    153       ismart:AraC_E_bind [T]
SQ   SEQUENCE   157 AA;  18081 MW;  C85BCD23F28C274E CRC64;
     MNYEIKQEEK RTVAGFHLVG PWEQTVKKGF EQLMMWVDSK NIVPKEWVAV YYDNPDETPA
     EKLRCDTVVT VPGYFTLPEN SEGVILTEIT GGQYAVAVAR VVGDDFAKPW YQFFNSLLQD
     SAYEMLPKPC FEVYLNNGAE DGYWDIEMYV AVQPKHH
//