ID FDNI_ECOLI Reviewed; 217 AA.
AC P0AEK7; P24185; P77513;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-NOV-2009, entry version 42.
DE RecName: Full=Formate dehydrogenase, nitrate-inducible, cytochrome b556(fdn) subunit;
DE AltName: Full=Formate dehydrogenase-N subunit gamma;
DE Short=FDH-N subunit gamma;
DE AltName: Full=Anaerobic formate dehydrogenase cytochrome b556 subunit;
GN Name=fdnI; OrderedLocusNames=b1476, JW1472;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=92042178; PubMed=1834669;
RA Berg B.L., Li J., Heider J., Stewart V.;
RT "Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I.
RT Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA)
RT encodes selenocysteine.";
RL J. Biol. Chem. 266:22380-22385(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX MEDLINE=97251357; PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
RA Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
RA Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
RA Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
RA Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome
RT corresponding to the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), AND METAL BINDING AT HIS-18;
RP HIS-57; HIS-155 AND HIS-169.
RX MEDLINE=21882259; PubMed=11884747; DOI=10.1126/science.1068186;
RA Jormakka M., Tornroth S., Byrne B., Iwata S.;
RT "Molecular basis of proton motive force generation: structure of
RT formate dehydrogenase-N.";
RL Science 295:1863-1868(2002).
CC -!- FUNCTION: Formate dehydrogenase allows E.coli to use formate as
CC major electron donor during anaerobic respiration, when nitrate is
CC used as electron acceptor. Subunit gamma is the cytochrome
CC b556(FDN) component of the formate dehydrogenase.
CC -!- COFACTOR: Binds 2 heme groups per subunit. Heme 1 is located at
CC the cytoplasmic interface, heme 2 is located at the periplasmic
CC interface. Electrons are transferred from the periplasmic to the
CC cytoplasmic heme.
CC -!- SUBUNIT: Trimer of heterotrimers, consisting of subunits alpha,
CC beta and gamma.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC protein.
CC -!- INDUCTION: By nitrate under anaerobic conditions.
CC -!- SIMILARITY: Belongs to the formate dehydrogenase gamma subunit
CC family.
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DR EMBL; M75029; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAD13440.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15125.1; -; Genomic_DNA.
DR PIR; G64900; JS0630.
DR RefSeq; AP_002099.1; -.
DR RefSeq; NP_415993.1; -.
DR PDB; 1KQF; X-ray; 1.60 A; C=1-217.
DR PDB; 1KQG; X-ray; 2.80 A; C=1-217.
DR PDBsum; 1KQF; -.
DR PDBsum; 1KQG; -.
DR STRING; P0AEK7; -.
DR TCDB; 5.A.3.2.1; prokaryotic molybdopterin-containing oxidoreductase (PMO) family.
DR GeneID; 946038; -.
DR GenomeReviews; AP009048_GR; JW1472.
DR GenomeReviews; U00096_GR; b1476.
DR KEGG; ecj:JW1472; -.
DR KEGG; eco:b1476; -.
DR EchoBASE; EB1211; -.
DR EcoGene; EG11229; fdnI.
DR HOGENOM; P0AEK7; -.
DR OMA; TWVEGSV; -.
DR BioCyc; EcoCyc:FDNI-MON; -.
DR BioCyc; MetaCyc:FDNI-MON; -.
DR Genevestigator; P0AEK7; -.
DR GO; GO:0009326; C:formate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0008863; F:formate dehydrogenase activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR GO; GO:0022904; P:respiratory electron transport chain; IEA:InterPro.
DR GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR InterPro; IPR006471; Formate_DH_gsu.
DR TIGRFAMs; TIGR01583; formate-DH-gamm; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Complete proteome;
KW Electron transport; Heme; Iron; Membrane; Metal-binding;
KW Transmembrane; Transport.
FT CHAIN 1 217 Formate dehydrogenase, nitrate-inducible,
FT cytochrome b556(fdn) subunit.
FT /FTId=PRO_0000087210.
FT TOPO_DOM 1 11 Cytoplasmic.
FT TRANSMEM 12 36
FT TOPO_DOM 37 52 Periplasmic.
FT TRANSMEM 53 74
FT TOPO_DOM 75 110 Cytoplasmic.
FT TRANSMEM 111 134
FT TOPO_DOM 135 150 Periplasmic.
FT TRANSMEM 151 175
FT TOPO_DOM 176 217 Cytoplasmic.
FT METAL 18 18 Iron (heme B 1 axial ligand).
FT METAL 57 57 Iron (heme B 2 axial ligand).
FT METAL 155 155 Iron (heme B 2 axial ligand).
FT METAL 169 169 Iron (heme B 1 axial ligand).
FT CONFLICT 160 217 IILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRW
FT YREIEKAEAKKESEEGI -> YHPDPRHPDPYVYGILGERI
FT D (in Ref. 1).
FT STRAND 4 8
FT HELIX 12 36
FT HELIX 41 45
FT HELIX 50 75
FT HELIX 76 78
FT HELIX 83 85
FT HELIX 86 90
FT HELIX 92 96
FT HELIX 100 103
FT HELIX 111 133
FT TURN 136 139
FT HELIX 140 142
FT HELIX 145 175
FT HELIX 179 184
FT STRAND 187 189
FT HELIX 190 196
FT HELIX 198 215
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 13 186 ipfam:Ni_hydr_CYTB [T]
SQ SEQUENCE 217 AA; 25368 MW; B10073F14343515E CRC64;
MSKSKMIVRT KFIDRACHWT VVICFFLVAL SGISFFFPTL QWLTQTFGTP QMGRILHPFF
GIAIFVALMF MFVRFVHHNI PDKKDIPWLL NIVEVLKGNE HKVADVGKYN AGQKMMFWSI
MSMIFVLLVT GVIIWRPYFA QYFPMQVVRY SLLIHAAAGI ILIHAILIHM YMAFWVKGSI
KGMIEGKVSR RWAKKHHPRW YREIEKAEAK KESEEGI
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