ID ENTA_ECOLI Reviewed; 248 AA.
AC P15047; P77100; Q2MBK5;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-NOV-2009, entry version 86.
DE RecName: Full=2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase;
DE EC=1.3.1.28;
GN Name=entA; OrderedLocusNames=b0596, JW0588;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-14.
RX MEDLINE=89123155; PubMed=2521622;
RA Liu J., Duncan K., Walsh C.T.;
RT "Nucleotide sequence of a cluster of Escherichia coli enterobactin
RT biosynthesis genes: identification of entA and purification of its
RT product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase.";
RL J. Bacteriol. 171:791-798(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=89123154; PubMed=2521621;
RA Nahlik M.S., Brickman T.J., Ozenberger B.A., McIntosh M.A.;
RT "Nucleotide sequence and transcriptional organization of the
RT Escherichia coli enterobactin biosynthesis cistrons entB and entA.";
RL J. Bacteriol. 171:784-790(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- CATALYTIC ACTIVITY: 2,3-dihydro-2,3-dihydroxybenzoate + NAD(+) =
CC 2,3-dihydroxybenzoate + NADH.
CC -!- PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis.
CC -!- SUBUNIT: Homooctamer.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC (SDR) family.
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DR EMBL; M24148; AAA16103.1; -; Unassigned_DNA.
DR EMBL; M24143; AAA76836.1; -; Genomic_DNA.
DR EMBL; U82598; AAB40796.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73697.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76351.1; -; Genomic_DNA.
DR PIR; A91904; DEECDB.
DR RefSeq; AP_001243.1; -.
DR RefSeq; NP_415128.1; -.
DR PDB; 2FWM; X-ray; 2.00 A; X=1-248.
DR PDBsum; 2FWM; -.
DR DIP; DIP:9511N; -.
DR STRING; P15047; -.
DR GeneID; 945284; -.
DR GenomeReviews; AP009048_GR; JW0588.
DR GenomeReviews; U00096_GR; b0596.
DR KEGG; ecj:JW0588; -.
DR KEGG; eco:b0596; -.
DR EchoBASE; EB0255; -.
DR EcoGene; EG10259; entA.
DR HOGENOM; P15047; -.
DR OMA; HASHITL; -.
DR BioCyc; EcoCyc:ENTA-MON; -.
DR BioCyc; MetaCyc:ENTA-MON; -.
DR Genevestigator; P15047; -.
DR GO; GO:0008667; F:2,3-dihydro-2,3-dihydroxybenzoate dehydroge...; IEA:EC.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009239; P:enterobactin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006826; P:iron ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR InterPro; IPR003560; DHB_DH.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR PANTHER; PTHR19410; ADH_short_C2; 1.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR01397; DHBDHDRGNASE.
DR PRINTS; PR00080; SDRFAMILY.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Direct protein sequencing;
KW Enterobactin biosynthesis; Ion transport; Iron; Iron transport; NAD;
KW Oxidoreductase; Transport.
FT CHAIN 1 248 2,3-dihydro-2,3-dihydroxybenzoate
FT dehydrogenase.
FT /FTId=PRO_0000054659.
FT NP_BIND 9 33 NAD (By similarity).
FT ACT_SITE 144 144 Proton acceptor (By similarity).
FT BINDING 131 131 Substrate (By similarity).
FT STRAND 7 12
FT HELIX 16 27
FT STRAND 31 37
FT STRAND 45 50
FT HELIX 56 69
FT STRAND 75 78
FT TURN 88 90
FT HELIX 93 103
FT HELIX 105 121
FT STRAND 125 129
FT HELIX 132 134
FT HELIX 142 162
FT HELIX 163 165
FT STRAND 168 174
FT HELIX 215 226
FT HELIX 228 230
FT STRAND 237 241
FT TURN 242 247
CC --------------------------------------------------------------------------
CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 131 159 ipat:ADH_SHORT [T]
SQ SEQUENCE 248 AA; 26250 MW; E840488335AD317B CRC64;
MDFSGKNVWV TGAGKGIGYA TALAFVEAGA KVTGFDQAFT QEQYPFATEV MDVADAAQVA
QVCQRLLAET ERLDALVNAA GILRMGATDQ LSKEDWQQTF AVNVGGAFNL FQQTMNQFRR
QRGGAIVTVA SDAAHTPRIG MSAYGASKAA LKSLALSVGL ELAGSGVRCN VVSPGSTDTD
MQRTLWVSDD AEEQRIRGFG EQFKLGIPLG KIARPQEIAN TILFLASDLA SHITLQDIVV
DGGSTLGA
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