ID Q9KVF9_VIBCH Unreviewed; 279 AA.
AC Q9KVF9;
DT 01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2000, sequence version 1.
DT 10-MAY-2017, entry version 75.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934};
DE EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
GN Name=rlmJ {ECO:0000256|HAMAP-Rule:MF_00934};
GN OrderedLocusNames=VC_0187 {ECO:0000313|EMBL:AAF93363.1};
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC Vibrionaceae; Vibrio.
OX NCBI_TaxID=243277 {ECO:0000313|EMBL:AAF93363.1, ECO:0000313|Proteomes:UP000000584};
RN [1] {ECO:0000313|EMBL:AAF93363.1, ECO:0000313|Proteomes:UP000000584}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961
RC {ECO:0000313|Proteomes:UP000000584};
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A.,
RA Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.,
RA Ermolaeva M.D., Vamathevan J., Bass S., Qin H., Dragoi I., Sellers P.,
RA McDonald L., Utterback T., Fleishmann R.D., Nierman W.C., White O.,
RA Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C.,
RA Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: Specifically methylates the adenine in position 2030 of
CC 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(2030) in 23S
CC rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S
CC rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SIMILARITY: Belongs to the RlmJ family. {ECO:0000256|HAMAP-
CC Rule:MF_00934}.
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DR EMBL; AE003852; AAF93363.1; -; Genomic_DNA.
DR PIR; B82353; B82353.
DR RefSeq; NP_229844.1; NC_002505.1.
DR RefSeq; WP_000954212.1; NC_002505.1.
DR ProteinModelPortal; Q9KVF9; -.
DR STRING; 243277.VC0187; -.
DR DNASU; 2614843; -.
DR EnsemblBacteria; AAF93363; AAF93363; VC_0187.
DR GeneID; 2614843; -.
DR KEGG; vch:VC0187; -.
DR PATRIC; 20079422; VBIVibCho83274_0170.
DR eggNOG; ENOG4105D6S; Bacteria.
DR eggNOG; COG2961; LUCA.
DR KO; K07115; -.
DR OMA; TYAIWYP; -.
DR BioCyc; VCHO:VC0187-MONOMER; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR HAMAP; MF_00934; 23SrRNA_methyltr_J; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR007473; RlmJ.
DR InterPro; IPR029063; SAM-dependent_MTases.
DR Pfam; PF04378; RsmJ; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000000584};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
KW Reference proteome {ECO:0000313|Proteomes:UP000000584};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_00934};
KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00934};
KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00934};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00934}.
FT REGION 143 144 S-adenosyl-L-methionine binding.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT ACT_SITE 164 164 Proton acceptor. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 19 19 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 42 42 S-adenosyl-L-methionine; via carbonyl
FT oxygen. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 100 100 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 118 118 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 164 164 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT SITE 4 4 Interaction with substrate rRNA.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
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CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 241 247 ipat:N6_MTASE [T]
FT MYHIT 33 277 ipfam:RsmJ [T]
FT MYHIT 2 266 ihamap:23SrRNA_methyltr_J [T]
SQ SEQUENCE 279 AA; 32287 MW; 38D08851047F942D CRC64;
MLSYRHSFHA GNHADVLKHI VQSLILNSLQ QKEKPFVYHD THSGVGRYDL THEWSEKTGE
YKQGIARIWQ QDNIPAELDS YLDAIRQLNQ GETLRYYPGS PRVARAHLRE QDRMVLTELH
PSDYPLLEQE FHRDRQVSIY KEDGFARLKA SLPPQERRGL VLIDPPYELA KEYRDVVRAI
AQSYKRWATG IYAIWYPVVN RCDIDDMLEG LQGLEIRKIL QIELGVAPDT NERGMTASGM
IVINPPWTLE SQMQTILPFL KQAIAPATGH YKVEWVVPE
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