ID Q9HUF0_PSEAE Unreviewed; 278 AA.
AC Q9HUF0;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 10-MAY-2017, entry version 79.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934};
DE EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
GN Name=rlmJ {ECO:0000256|HAMAP-Rule:MF_00934};
GN OrderedLocusNames=PA5019 {ECO:0000313|EMBL:AAG08404.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
OS JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964 {ECO:0000313|EMBL:AAG08404.1, ECO:0000313|Proteomes:UP000002438};
RN [1] {ECO:0000313|EMBL:AAG08404.1, ECO:0000313|Proteomes:UP000002438}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC 1C / PRS 101 / PAO1 {ECO:0000313|Proteomes:UP000002438};
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.Q., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.,
RA Smith K., Spencer D., Wong G.K., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H., Hancock R.E., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT opportunistic pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Specifically methylates the adenine in position 2030 of
CC 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(2030) in 23S
CC rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S
CC rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SIMILARITY: Belongs to the RlmJ family. {ECO:0000256|HAMAP-
CC Rule:MF_00934}.
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DR EMBL; AE004091; AAG08404.1; -; Genomic_DNA.
DR PIR; C83019; C83019.
DR RefSeq; NP_253706.1; NC_002516.2.
DR RefSeq; WP_003114560.1; NZ_ASJY01000798.1.
DR ProteinModelPortal; Q9HUF0; -.
DR SMR; Q9HUF0; -.
DR STRING; 208964.PA5019; -.
DR PaxDb; Q9HUF0; -.
DR DNASU; 881241; -.
DR EnsemblBacteria; AAG08404; AAG08404; PA5019.
DR GeneID; 881241; -.
DR KEGG; pae:PA5019; -.
DR PATRIC; 19844865; VBIPseAer58763_5261.
DR PseudoCAP; PA5019; -.
DR eggNOG; ENOG4105D6S; Bacteria.
DR eggNOG; COG2961; LUCA.
DR HOGENOM; HOG000262479; -.
DR InParanoid; Q9HUF0; -.
DR KO; K07115; -.
DR OMA; TYAIWYP; -.
DR PhylomeDB; Q9HUF0; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR HAMAP; MF_00934; 23SrRNA_methyltr_J; 1.
DR InterPro; IPR007473; RlmJ.
DR InterPro; IPR029063; SAM-dependent_MTases.
DR Pfam; PF04378; RsmJ; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000002438};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
KW Reference proteome {ECO:0000313|Proteomes:UP000002438};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_00934};
KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00934};
KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00934};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00934}.
FT REGION 142 143 S-adenosyl-L-methionine binding.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT ACT_SITE 163 163 Proton acceptor. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 18 18 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 41 41 S-adenosyl-L-methionine; via carbonyl
FT oxygen. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 99 99 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 117 117 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 163 163 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT SITE 3 3 Interaction with substrate rRNA.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
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CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 32 276 ipfam:RsmJ [T]
FT MYHIT 1 265 ihamap:23SrRNA_methyltr_J [T]
SQ SEQUENCE 278 AA; 31882 MW; 2387E7CA20706572 CRC64;
MNYRHAYHAG NHADVFKHLV LARIFALLGR KDTPYAYLDS HSGVGLYDLQ GDEASRTGEW
EQGIARLWER EGLPEQLDDY LDVIRHLNPD GSLRYYPGSP ELARRLTRAQ DRVLLNELHP
EDGRLLKANM AGERRVAVHQ GDGWLLPRAF LPAPEKRAVL LIDPPFEQAD ELQRCVAALD
EAIGRMRQTV VVIWYPIKDQ RQLKRFYQAL ERSVAPKLLR AELHVHPIDD AERFNGSGLA
IVNPPWPLED ELRDLLPWLA EVMAQSQGGW RLDWLVAE
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