ID Q9A998_CAUCR Unreviewed; 273 AA.
AC Q9A998;
DT 01-JUN-2001, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2001, sequence version 1.
DT 10-MAY-2017, entry version 75.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934};
DE EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
GN Name=rlmJ {ECO:0000256|HAMAP-Rule:MF_00934};
GN OrderedLocusNames=CC_1096 {ECO:0000313|EMBL:AAK23080.1};
OS Caulobacter crescentus (strain ATCC 19089 / CB15).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=190650 {ECO:0000313|EMBL:AAK23080.1, ECO:0000313|Proteomes:UP000001816};
RN [1] {ECO:0000313|EMBL:AAK23080.1, ECO:0000313|Proteomes:UP000001816}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15 {ECO:0000313|Proteomes:UP000001816};
RX PubMed=11259647; DOI=10.1073/pnas.061029298;
RA Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA Eisen J.A., Heidelberg J.F., Alley M.R., Ohta N., Maddock J.R.,
RA Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H.,
RA Kolonay J.F., Smit J., Craven M.B., Khouri H., Shetty J., Berry K.,
RA Utterback T., Tran K., Wolf A., Vamathevan J., Ermolaeva M., White O.,
RA Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT "Complete genome sequence of Caulobacter crescentus.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC -!- FUNCTION: Specifically methylates the adenine in position 2030 of
CC 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(2030) in 23S
CC rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S
CC rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SIMILARITY: Belongs to the RlmJ family. {ECO:0000256|HAMAP-
CC Rule:MF_00934}.
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DR EMBL; AE005673; AAK23080.1; -; Genomic_DNA.
DR PIR; D87385; D87385.
DR RefSeq; NP_419912.1; NC_002696.2.
DR RefSeq; WP_010918980.1; NC_002696.2.
DR ProteinModelPortal; Q9A998; -.
DR SMR; Q9A998; -.
DR STRING; 190650.CC_1096; -.
DR EnsemblBacteria; AAK23080; AAK23080; CC_1096.
DR GeneID; 941522; -.
DR KEGG; ccr:CC_1096; -.
DR PATRIC; 21299174; VBICauCre124313_1115.
DR eggNOG; ENOG4105D6S; Bacteria.
DR eggNOG; COG2961; LUCA.
DR HOGENOM; HOG000262479; -.
DR KO; K07115; -.
DR OMA; TYAIWYP; -.
DR BioCyc; CAULO:CC1096-MONOMER; -.
DR Proteomes; UP000001816; Chromosome.
DR GO; GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0070475; P:rRNA base methylation; IEA:UniProtKB-HAMAP.
DR HAMAP; MF_00934; 23SrRNA_methyltr_J; 1.
DR InterPro; IPR007473; RlmJ.
DR InterPro; IPR029063; SAM-dependent_MTases.
DR Pfam; PF04378; RsmJ; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000001816};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
KW Reference proteome {ECO:0000313|Proteomes:UP000001816};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_00934};
KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00934};
KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00934};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00934}.
FT REGION 142 143 S-adenosyl-L-methionine binding.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT ACT_SITE 162 162 Proton acceptor. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 18 18 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 41 41 S-adenosyl-L-methionine; via carbonyl
FT oxygen. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 99 99 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 117 117 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 162 162 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT SITE 3 3 Interaction with substrate rRNA.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
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CC The following FT lines are automated annotations from the MyHits database.
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FT MYHIT 1 263 ihamap:23SrRNA_methyltr_J [T]
FT MYHIT 33 272 ipfam:RsmJ [T]
SQ SEQUENCE 273 AA; 29251 MW; 9172D6E0AF564202 CRC64;
MNYRHAFHAG NFADLHKHAI LLAMLSALQE KSPALAVIDT HAGAGGYDLA GEMARRSGEA
QAGIFRLKAA ADAPAVFQPL LNAITQMNGG KDGDLYPGSP RLMARALRGA DRYVGCELRD
DDADLLRKTL APCANARALQ ADGFDTAVKD AGKGGRAFIV IDPPFERPDD YDRIVATTRA
VLARAPDAAL AIWLPIKDLE TFDAFLRAME TVTSDLLVAE LRLRPLTDPM KMNGCAMVMI
GAPPSVEDAA VAAGDWIATR LGEPGGRSRV WRT
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