ID Q88D82_PSEPK Unreviewed; 278 AA.
AC Q88D82;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 10-MAY-2017, entry version 70.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934};
DE EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
DE AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
GN Name=rlmJ {ECO:0000256|HAMAP-Rule:MF_00934,
GN ECO:0000313|EMBL:AAN70512.1};
GN OrderedLocusNames=PP_4945 {ECO:0000313|EMBL:AAN70512.1};
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 /
OS KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488 {ECO:0000313|EMBL:AAN70512.1, ECO:0000313|Proteomes:UP000000556};
RN [1] {ECO:0000313|EMBL:AAN70512.1, ECO:0000313|Proteomes:UP000000556}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
RC {ECO:0000313|Proteomes:UP000000556};
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L., Beanan M., DeBoy R.T., Daugherty S., Kolonay J.,
RA Madupu R., Nelson W., White O., Peterson J., Khouri H., Hance I.,
RA Chris Lee P., Holtzapple E., Scanlan D., Tran K., Moazzez A.,
RA Utterback T., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S.,
RA Kiewitz C., Eisen J.A., Timmis K.N., Dusterhoft A., Tummler B.,
RA Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the
RT metabolically versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- FUNCTION: Specifically methylates the adenine in position 2030 of
CC 23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(2030) in 23S
CC rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S
CC rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC -!- SIMILARITY: Belongs to the RlmJ family. {ECO:0000256|HAMAP-
CC Rule:MF_00934}.
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DR EMBL; AE015451; AAN70512.1; -; Genomic_DNA.
DR RefSeq; NP_747048.1; NC_002947.4.
DR RefSeq; WP_010955521.1; NC_002947.4.
DR ProteinModelPortal; Q88D82; -.
DR STRING; 160488.PP_4945; -.
DR GeneID; 1043921; -.
DR KEGG; ppu:PP_4945; -.
DR PATRIC; 19948700; VBIPsePut30601_5279.
DR eggNOG; ENOG4105D6S; Bacteria.
DR eggNOG; COG2961; LUCA.
DR HOGENOM; HOG000262479; -.
DR KO; K07115; -.
DR OMA; TYAIWYP; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0070475; P:rRNA base methylation; IEA:UniProtKB-HAMAP.
DR HAMAP; MF_00934; 23SrRNA_methyltr_J; 1.
DR InterPro; IPR007473; RlmJ.
DR InterPro; IPR029063; SAM-dependent_MTases.
DR Pfam; PF04378; RsmJ; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000000556};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934,
KW ECO:0000313|EMBL:AAN70512.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000556};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_00934};
KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00934};
KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00934};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00934,
KW ECO:0000313|EMBL:AAN70512.1}.
FT REGION 142 143 S-adenosyl-L-methionine binding.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT ACT_SITE 163 163 Proton acceptor. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 18 18 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 41 41 S-adenosyl-L-methionine; via carbonyl
FT oxygen. {ECO:0000256|HAMAP-
FT Rule:MF_00934}.
FT BINDING 99 99 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 117 117 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT BINDING 163 163 S-adenosyl-L-methionine.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
FT SITE 3 3 Interaction with substrate rRNA.
FT {ECO:0000256|HAMAP-Rule:MF_00934}.
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CC The following FT lines are automated annotations from the MyHits database.
CC --------------------------------------------------------------------------
FT MYHIT 1 265 ihamap:23SrRNA_methyltr_J [T]
FT MYHIT 32 275 ipfam:RsmJ [T]
SQ SEQUENCE 278 AA; 31851 MW; DBD40D9C524DFCD0 CRC64;
MNYRHAFHAG NHADVLKHIV LTRLIALMSR KEQPFAYIDT HAGLGLYDLQ GDQASRTGEY
LEGVARLWNR DDLPAMADDY LRIIKRLNAD GELRYYPGSP ELARRLMRQQ DRALLNEKHP
EDGPLLKENM KKDPRVTVHL GEGWHVPRAL LPVQEKRAIM LIDPPFEQAD ELKRCTTAMK
EAIGRMRQTV AAIWYPIKDQ RSLTRYYQDL TSTGAPKLLR VELYVHHQDS PQGLNGSGLA
IANPPWGLED ELKELLPWLA KELAQTAGSY RMDWLIAE
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