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Pagni M, Ioannidis V, Cerutti L, Zahn-Zabal M, Jongeneel CV, Hau J, Martin O, Kuznetsov D, Falquet L.
MyHits: improvements to an interactive resource for analyzing protein sequences.
Nucleic Acids Res. 2007 Jul; 35(Web Server issue):W433-7

DescriptionRecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934}; EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934}; AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934}; AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
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MyHits synonymsQ7CPJ1_SALTY , Q7CPJ1 , 2F2F01425253100F
match map segment
ipfam:RsmJ ihamap:23SrRNA_methyltr_J ipat:N6_MTASE  
Legends: 1, ACT_SITE Proton acceptor. {ECO:0000256|HAMAP- Rule:MF_00934}; 2, BINDING S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_00934}; 3, BINDING S-adenosyl-L-methionine; via carbonyl oxygen. {ECO:0000256|HAMAP- Rule:MF_00934}; 4, SITE Interaction with substrate rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}; 5, REGION S-adenosyl-L-methionine binding. {ECO:0000256|HAMAP-Rule:MF_00934}; 6, ipat:N6_MTASE [T]. 
ID   Q7CPJ1_SALTY            Unreviewed;       280 AA.
AC   Q7CPJ1;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   10-MAY-2017, entry version 67.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934};
DE            EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934};
DE   AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
DE   AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
GN   Name=yhiR {ECO:0000313|EMBL:AAL22456.1};
GN   Synonyms=rlmJ {ECO:0000256|HAMAP-Rule:MF_00934};
GN   OrderedLocusNames=STM3596 {ECO:0000313|EMBL:AAL22456.1};
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287 {ECO:0000313|EMBL:AAL22456.1, ECO:0000313|Proteomes:UP000001014};
RN   [1] {ECO:0000313|EMBL:AAL22456.1, ECO:0000313|Proteomes:UP000001014}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720
RC   {ECO:0000313|Proteomes:UP000001014};
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
RA   Waterston R., Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium
RT   LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Specifically methylates the adenine in position 2030 of
CC       23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(2030) in 23S
CC       rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S
CC       rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC   -!- SIMILARITY: Belongs to the RlmJ family. {ECO:0000256|HAMAP-
CC       Rule:MF_00934}.
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DR   EMBL; AE006468; AAL22456.1; -; Genomic_DNA.
DR   RefSeq; NP_462497.1; NC_003197.2.
DR   RefSeq; WP_000954235.1; NC_003197.2.
DR   ProteinModelPortal; Q7CPJ1; -.
DR   STRING; 99287.STM3596; -.
DR   PaxDb; Q7CPJ1; -.
DR   PRIDE; Q7CPJ1; -.
DR   EnsemblBacteria; AAL22456; AAL22456; STM3596.
DR   GeneID; 1255119; -.
DR   KEGG; stm:STM3596; -.
DR   PATRIC; 32386005; VBISalEnt20916_3800.
DR   eggNOG; ENOG4105D6S; Bacteria.
DR   eggNOG; COG2961; LUCA.
DR   HOGENOM; HOG000262479; -.
DR   KO; K07115; -.
DR   OMA; TYAIWYP; -.
DR   PhylomeDB; Q7CPJ1; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR   HAMAP; MF_00934; 23SrRNA_methyltr_J; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR007473; RlmJ.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   Pfam; PF04378; RsmJ; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000001014};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001014};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_00934};
KW   rRNA processing {ECO:0000256|HAMAP-Rule:MF_00934};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00934};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   REGION      143    144       S-adenosyl-L-methionine binding.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   ACT_SITE    164    164       Proton acceptor. {ECO:0000256|HAMAP-
FT                                Rule:MF_00934}.
FT   BINDING      19     19       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   BINDING      42     42       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen. {ECO:0000256|HAMAP-
FT                                Rule:MF_00934}.
FT   BINDING     100    100       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   BINDING     118    118       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   BINDING     164    164       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   SITE          4      4       Interaction with substrate rRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
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CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT        33    278       ipfam:RsmJ [T]
FT   MYHIT         2    266       ihamap:23SrRNA_methyltr_J [T]
FT   MYHIT       241    247       ipat:N6_MTASE [T]
SQ   SEQUENCE   280 AA;  32137 MW;  2F2F01425253100F CRC64;
     MLSYRHSFHA GNHADVLKHT VQSLIIESLK EKEKPFLYLD THAGAGRYQL GSEHAERTGE
     YLEGIARIWQ QDDLPAELEP YISVVKHFNR SGQLRYYPGS PLIARQLLRE QDSLQLTELH
     PSDFPLLRAE FQKDNRARVE RADGYQQLKA KLPPVSRRGL ILIDPPYEMK TDYQAVVSGI
     SEGYKRFATG TYALWYPVVL RQQIKRMIHE LEATGIRKIL QIELAIRPDS DQRGMTASGM
     IVVNPPWKLE QQMNNVLPWL HSRLAPNGHG HTSVSWIVPE
//