Entry bact:Q7CG06_YERPE

ID   Q7CG06_YERPE            Unreviewed;       280 AA.
AC   Q7CG06; Q74QW5;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   10-MAY-2017, entry version 88.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase J {ECO:0000256|HAMAP-Rule:MF_00934};
DE            EC=2.1.1.266 {ECO:0000256|HAMAP-Rule:MF_00934};
DE   AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
DE   AltName: Full=23S rRNA m6A2030 methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
GN   Name=rlmJ {ECO:0000256|HAMAP-Rule:MF_00934};
GN   OrderedLocusNames=YPO3976 {ECO:0000313|EMBL:CAL22556.1};
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=632 {ECO:0000313|Proteomes:UP000000815};
RN   [1] {ECO:0000313|EMBL:CAL22556.1, ECO:0000313|Proteomes:UP000000815}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis {ECO:0000313|Proteomes:UP000000815};
RX   PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G.,
RA   Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S.,
RA   Karlyshev A.V., Moule S., Oyston P.C.F., Quail M., Rutherford K.,
RA   Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
CC   -!- FUNCTION: Specifically methylates the adenine in position 2030 of
CC       23S rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(2030) in 23S
CC       rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S
CC       rRNA. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00934}.
CC   -!- SIMILARITY: Belongs to the RlmJ family. {ECO:0000256|HAMAP-
CC       Rule:MF_00934}.
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DR   EMBL; AL590842; CAL22556.1; -; Genomic_DNA.
DR   PIR; AI0483; AI0483.
DR   RefSeq; WP_002209533.1; NZ_LT605018.1.
DR   RefSeq; YP_002348846.1; NC_003143.1.
DR   STRING; 187410.y3853; -.
DR   DNASU; 1148800; -.
DR   EnsemblBacteria; AAM87398; AAM87398; y3853.
DR   EnsemblBacteria; AAS63504; AAS63504; YP_3339.
DR   GeneID; 1176814; -.
DR   KEGG; ype:YPO3976; -.
DR   KEGG; ypj:CH55_2655; -.
DR   KEGG; ypk:y3853; -.
DR   KEGG; ypl:CH46_1081; -.
DR   KEGG; ypm:YP_3339; -.
DR   KEGG; ypv:BZ15_3729; -.
DR   KEGG; ypw:CH59_1924; -.
DR   eggNOG; ENOG4105D6S; Bacteria.
DR   eggNOG; COG2961; LUCA.
DR   HOGENOM; HOG000262479; -.
DR   KO; K07115; -.
DR   OMA; TYAIWYP; -.
DR   Proteomes; UP000000815; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR   HAMAP; MF_00934; 23SrRNA_methyltr_J; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR007473; RlmJ.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   Pfam; PF04378; RsmJ; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000000815};
KW   Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00934};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000815};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_00934};
KW   rRNA processing {ECO:0000256|HAMAP-Rule:MF_00934};
KW   S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00934};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   REGION      143    144       S-adenosyl-L-methionine binding.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   ACT_SITE    164    164       Proton acceptor. {ECO:0000256|HAMAP-
FT                                Rule:MF_00934}.
FT   BINDING      19     19       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   BINDING      42     42       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen. {ECO:0000256|HAMAP-
FT                                Rule:MF_00934}.
FT   BINDING     100    100       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   BINDING     118    118       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   BINDING     164    164       S-adenosyl-L-methionine.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
FT   SITE          4      4       Interaction with substrate rRNA.
FT                                {ECO:0000256|HAMAP-Rule:MF_00934}.
CC   --------------------------------------------------------------------------
CC   The following FT lines are automated annotations from the MyHits database.
CC   --------------------------------------------------------------------------
FT   MYHIT         2    266       ihamap:23SrRNA_methyltr_J [T]
FT   MYHIT       241    247       ipat:N6_MTASE [T]
FT   MYHIT        33    278       ipfam:RsmJ [T]
SQ   SEQUENCE   280 AA;  32304 MW;  F6FC38B7843AFD19 CRC64;
     MLSYRHSFHA GNHADVLKHT VQSLIIEAMK EKEKPFLYLD THAGAGRYQL SGEHAERTGE
     YLDGIGKLWQ RDDLPADLAP YMSAINYFNR GEKLRYYPGS PLIARHLLRE DDKIHLTELH
     SSDYPLLRNE FAKDERAKVQ RADGYQQLKS QLPPLSRRGF VLIDPPYEMK TDYQDVVKGI
     QEGYKRFATG TYALWYPVVL RQQIKRLLRD LEATGIRRIL QIELAVRPDS DQHGMTASGM
     IVINPPWKLE QQMNSLLPWL HNVLVPSGHG HTLVKWVVPE
//